《鱼Cortisol试剂盒ELISA定量检测》由会员分享，可在线阅读，更多相关《鱼Cortisol试剂盒ELISA定量检测（2页珍藏版）》请在金锄头文库上搜索。
1、鱼Cortisol试剂盒,ELISA定量检测Fish Cortisol kit, ELISA quantitative detection检测原理采用双抗体夹心ABC-ELISA法所需样本体积50-100ul试剂盒成分酶标板，试剂，标准品等检测波长450 nm1, high efficiency, sensitive and specific antibody;2, stable repeatability and reliability;3, adsorption performance is good, the blank value is low, high transparency i
2、n the bottom of the bore of the solid phase carrier;4, apply the serum, plasma and tissue of grout, cell culture supernatant, urine, and so on a variety of samples;5, maximum limit save experimental funds instructions1. Kit to save: - 20 C (longer) when not in use; 2-8 C (frequently) when using.2. S
3、trong detergents cryogenics will be a salting out, when diluted in water bath heating to dissolve.3. The Chinese and English instruction may have discrepancy, please refer to our English instruction.4. Just open the enzyme-linked board may contain a little watery material in the hole, this is a norm
4、al phenomenon, will not have any impact on the experimental results 鱼 Corti so1 剂盒，ELISA 定检测 Test principle:Experiment TRAb kit is indirect enzyme-linked immunosorbent (ELISA). Known to TRAb standard, the concentration of the unknown sample concentration while adding the microporous enzyme label pla
5、te for testing. First the TRAb and biotin labeled antibody incubate at the same time. After washing, add avidin tagged HRP. Again after incubate and washing, the enzyme that removes not combine, then add in the substrate of A and B, and the enzyme combination at the same time. Color is produced. Col
6、or depth and sample concentrations of TRAb with proportional relationship.Service advantage: provide technical guidance and ELISA experiment all the ELISA test kit for free generation鱼Cortisol试剂盒,ELISA定量检测洗板方法手工洗板方法：吸去（不可触及板壁）或甩掉酶标板内的液体；在实验台上铺垫几层吸水纸，酶标板朝下 用力拍几次；将30（ 48T的20倍）倍浓缩洗涤液用蒸馏水30（ 48T的20倍）倍稀释后备用。根据需要， 重复此过程数次。自动洗板：如果有自动洗板机，应在熟练使用后再用到正式实验过程中。鱼Corti so1试剂盒,ELISA定量检测实验结果计算以所测标准品的od值为横坐标，标准品的浓度值为纵坐标，在坐标纸上或用相关软件绘制标准曲线，并得到直线回归方程，将样品的OD值代入 方程，计算出样品的浓度。（此图仅供参考）鱼皮质醇（Cortisol ） ELISA试剂盒性能1. 检测范围：50 pg/mL 一 1600 pg/mL。2. 灵敏度：最低检测浓度小于10 pg/mL。3. 特异性：不与其它可溶性结构类似物交叉反应。4. 重复性：板内变异系数小于10%，板间变异系数小于15%。
