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1、Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatographymass spectrometry Haifeng Wua, , Jian Guob, , Shilin Chena, , Xin Liuc, , Yan Zhoud, , Xiaopo Zhanga, , Xudong Xua, , a Institute of Medicinal Plant Development
2、, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, PR China b Department of DMPK, AstraZeneca Pharmaceuticals, 35 Gatehouse Drive, Waltham, MA 02451, USA c Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, PR China d Chengdu Institute of Biolog
3、y, Chinese Academy of Sciences, Chengdu 610041, PR ChinaCorresponding author at: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No.151, Malianwa North Road, Haidian District, Beijing 100193, PR China. Tel.: +86 10 5783 3296; fax: +86 1
4、0 5783 3296.Received 14 May 2012Revised 30 August 2012Accepted 2 September 2012Available online 11 September 2012 http:/dx.doi.org/10.1016/j.jpba.2012.09.004, How to Cite or Link Using DOI Permissions & ReprintsView full text Purchase$31.501. Introduction2. Qualitative analysis3. Quantitative analys
5、is4. Application of LCMS in fingerprinting analysis5. ConclusionsAcknowledgmentsReferencesAbstractOver the past few years, the applications of liquid chromatography coupled with mass spectrometry (LCMS) in natural product analysis have been dramatically growing because of the increasingly improved s
6、eparation and detection capabilities of LCMS instruments. In particular, novel high-resolution hybrid instruments linked to ultra-high-performance LC and the hyphenations of LCMS with other separation or analytical techniques greatly aid unequivocal identification and highly sensitive quantification
7、 of natural products at trace concentrations in complex matrices. With the aim of providing an up-to-date overview of LCMS applications on the analysis of plant-derived compounds, papers published within the latest years (20072012) involving qualitative and quantitative analysis of phytochemical con
8、stituents and their metabolites are summarized in the present review. After briefly describing the general characteristics of natural products analysis, the most remarkable features of LCMS and sample preparation techniques, the present paper mainly focuses on screening and characterization of pheno
9、ls (including flavonoids), alkaloids, terpenoids, steroids, coumarins, lignans, and miscellaneous compounds in respective herbs and biological samples, as well as traditional Chinese medicine (TCM) prescriptions using tandem mass spectrometer. Chemical fingerprinting analysis using LCMS is also desc
10、ribed. Meanwhile, instrumental peculiarities and methodological details are accentuated.Keywords Liquid chromatography; Mass spectrometry; Medicinal plants; Traditional Chinese medicine; Fingerprinting抽象在过去的几年中，液相色谱与质谱联用（LC-MS）在天然产物分析的应用都得到了显着增长，因为日益提高的分离和检测能力的LC-MS仪器。特别是，新颖的高分辨率混合工具与其他分离或分析技术链接到超高性
11、能LC的LC-MS和断字大大有助于在复杂基质中的微量浓度的明确识别和天然产品的高度敏感的定量。提供最新的植物性化合物的分析概述LC-MS应用的目的，论文发表在最近几年（2007-2012年），涉及的植物化学成分及其代谢物的定性和定量分析总结在本次审查中。在简要说明天然产物的分析，最显着的特点LC-MS和样品制备技术的一般特点，本论文主要集中在酚类物质（包括类黄酮），生物碱，萜类化合物，类固醇，香豆素，木脂素类化合物的筛选和鉴定，和杂项在各自的草药化合物和生物样品，以及中国传统医学（中医）处方使用串联质谱仪。化学指纹分析用LC-MS也被描述。同时，仪器的特点和方法的细节突出。Figures an
12、d tables from this article:Fig. 1.Chemical structures of caged xanthones (115) identified from G. hanburyi.Reproduced with permission from 89. 氧杂蒽酮的化学结构（1-15）确定Figure options View in workspaceFig. 2.UHPLCMS chromatograms of the standard xanthones and the crude extracts of G. hanburyi. (A) UHPLC chro
13、matograms of 12 standard xanthones, (B) TIC of the 12 standard xanthones, (C) UHPLC chromatograms of the crude extracts of G. hanburyi, (D) TIC of the crude extracts of G. hanburyi. 。2。UHPLC-MS色谱图的标准呫吨酮和G. hanburyi粗提物中。 （A）（B）UHPLC色谱图的12个标准氧杂蒽酮，TIC的12个标准氧杂蒽酮（C）UHPLC色谱G. hanburyi粗提物中，（D）议会的粗提物的G. han
14、buryi。Reproduced with permission from 89.Figure options View in workspaceFig. 3.(A) MS/MS spectra of isogambogenin (13) of selected M+H+ at m/z 615, (B) MS/MS/MS spectra of selected ion at m/z 491 from m/z 615, (C) MS/MS spectra of isomorellinol (14) of selected M+H+ at m/z 547, (D) MS/MS/MS spectra
15、 of selected ion at m/z 461 from m/z 547, (E) MS/MS spectra of compound 15 of selected M+H+ at m/z 545, (F) MS/MS/MS spectra of selected ion at m/z 461 from m/z 545 (collision energy at 30eV).图。3。（A）MS /，MS谱isogambogenin（13）选定的M+ H + m / z为615，（B）选择离子从m / z615，m / z为491 MS / MS / MS谱（C）MS / MS，光谱isomorellinol（14）选择M+ H + m / z为547（D）MS / MS / MS谱的选择离子为m / z461 M / Z547，（E）MS/ MS谱的化合物15，所选的M + H+为m/ z545（F）MS / MS/ MS谱图，从m / z545（碰撞能量30 eV的选择离子为m / z461）。Reproduced with permission from 89.Figure options View in workspaceFig. 4.Proposed fragment pathways of M+H+ for: (A) isogambogen
