《0.1mPBS缓冲液的配制.doc》由会员分享，可在线阅读，更多相关《0.1mPBS缓冲液的配制.doc（3页珍藏版）》请在金锄头文库上搜索。
25下0.1mol/L磷酸钾缓冲液的配制pH1mol/L K2HPO4(ml)1mol/L KH2PO4(ml)5.88.591.56.013.286.86.219.280.86.427.872.26.638.161.96.849.750.37.061.538.57.271.728.37.480.219.87.686.613.47.890.89.28.094.06.2（2）25下0.1mol/L磷酸钠缓冲液的配制pH1mol/L Na2HPO4(ml)1mol/L NaH2PO4(ml)5.87.992.16.012.088.06.217.882.26.425.574.56.635.264.86.846.353.77.057.742.37.268.431.67.477.422.67.684.515.57.889.610.48.093.26.8:用蒸馏水将混合的两种1mol/L贮存液稀释至1000ml，根据Henderson-Hasselbalch方程计算其pH值：pH=pK+1g(质子受体/质子供体)在此，pK=6.86(25)。
