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1、Section A - Cells and macromolecules 1The glycosylation of secreted proteins takes place in the . . .A mitochondria. B peroxisomes. C endoplasmic reticulum. D nucleus. 2Which of the following is an example of a nucleoprotein?A keratin. B chromatin. C histone. D proteoglycan.3Which of the following i
2、s not a polysaccharide? A chitin. B amylopectin. C glycosaminoglycan.D glycerol. 4. Transmembrane proteinsA join two lipid bilayers together. B have intra- and extracellular domains. C are contained completely within the membrane. D are easily removed from the membrane.Section B - Protein structure1
3、. Which of the following is an imino acid? A proline. B hydroxy lysine. C tryptophan. D histidine. 2 Protein family members in different species that carry out the same biochemical role are described as . . . A paralogs. B structural analogs. C heterologs. D orthologs. 3. Which of the following is n
4、ot a protein secondary structure?A -helix.B triple helix. C double helix. D -pleated sheet. 4 In isoelectric focusing, proteins are separated .A in a pH gradient. B in a salt gradient. C in a density gradient. D in a temperature gradient.5Edman degradation sequences peptides . . . A using a cDNA seq
5、uence. B according to their masses. C From the C-terminus to the N-terminus. D from the N-terminus to the C-terminus. Section C - properties of nucleic acids1The sequence 5-AGTCTGACT-3 in DNA is equivalent to which sequence in RNA? A 5-AGUCUGUGACU -3 B 5 -UGTCTGUTC -3 C 5 -UCAGUCUGA-3 D 5- AGUCAGACU
6、-3 2. Which of the following correctly describes A-DNA? A a right-handed antiparallel double helix with 10 bp/turn and bases lying perpendicular to the helix axis. B a left-handed antiparallel double-helix with 12 bp/turn formed from alternating pyrimidine-purine sequences. C a right-handed antipara
7、llel double helix with 11 bp/turn and bases tilted with respect to the helix axis. D a globular structure formed by short intramolecular helices formed in a single-strand nucleic acid. 3. Denaturation of double stranded DNA involves.A preakage into short double-stranded fragments.B separation into s
8、ingle strands. C hydrolysis of the DNA backbone.D cleavage of the bases from the sugar-phosphate backbone. 4. Which has the highest absorption per unit mass at a wavelength of 260 nm?A double-stranded DNA. B mononucleotides. C RNA. D protein. 5. Type I DNA topoisomeraes .A change linking number by士2
9、 B require ATP. C break one strand of a DNA double helix. D are the target of antibacterial drugs. Section D - Prokaryotic and eukaryotic chromatin structure1Which of the following is common to both E. coli and eukaryotic chromosomes? A the DNA is circular. B the DNA is packaged into nucleosomes.C t
10、he DNA is contained in the nucleus. D the DNA is negatively supercoiled. 2A compl of 166 bp of DNA with the histone octamer plus histone HI is known as a . . . A nucleosome core. B solenoid. C 30 nm fiber. D chromatosome. 3In what region of the interphase chromosome does transcription take place? A
11、the telomere. B the centromere. C euchromatin. D heterochromatin. 4 Which statement about CpG islands and methylation is not true? A CpG islands are particularly resistant to DNase I. B CpG methylation is responsible for the mutation of CpG to TpG in eukaryotes. C CpG islands occur around the promot
12、ers of active genes. D CpG methylation is associated with inactive chromatin. 5Which of the following is an example of highly-repetitive DNA? A Alu element. B histone gene cluster. C DNA minisatellites. D dispersed repetitive DNA. Section E - DNA replication1The number of replicons in a typical mamm
13、alian cell is . . . A 40-200. B 400. C 1000-2000. D 50000-100000. 2. In prokaryotes,the lagging strand primers are removed by . . . A 3 to 5 exonuclease. B DNA ligase. C DNA polymerase I. D DNA polymerase III. 3. The essential initiator protein at the E. coli origin of replication is . . . A DnaA. B
14、 DnaB. C DnaC. D DnaE. 4. Which phase would a cell enter if it was starved of mitogens before the R point? A G1. B S. C G2. D G0. 5. Which one of the following statements is true? A once the cell has passed the R point, cell division is inevitable. B the phosphorylation of Rb by a G1 cyclin-CDK complex is a critical requirement for entry into S phase . C phosphorylation of E2F by a G1 cyclin-CDK complex is a critical requirement for entry into S phase. D cyclin D1 and INK4 p16 are tumor suppressor proteins. 6. In eukaryotes, euchromatin replicates predominantly. A in early S-phase. B in mid
