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1、清华大学XX年生物化学本科期末考试试题考试科目：生物化学考试时间:考试类型：期末试题I.1： howmany crbs dsAraiiacid ave?（2 carons)2: o mny dubleondoesAachdoni achave? (4douleds）3: list two advantaes thtas have ovrugars as ored fus （reeeg ram fr gam; ohyan needed)4: whre isieth els amost of the hspholpidsdrad（lyoomes)5: olgoschardehad rup dete
2、rine t blood type fa iniiual.o are thattcd to th plasma ebrane?(glyoshinglpid or lipds ad sface protns)6:istatleast oe gentic deasethaculdresultfom bnorma ccumatio ofmbrne liis （Tay-as, andhoffs, abys, aucs, r Niann-Pickdises）7: is the thremai ecoanids that poducdfom ridni aci (rotands； hromoxanes;
3、and letriene).8: is ne AD now (asin, ibupofen,o acetamnohenor mclfenamat)9: liswo fatsollevtais (, D, E， K)1:wih im can e drivd rom beta-carotene (A).11: whchyearwas th flui moaic me oposd? (192)12：why he thickes f ost biological mebrs s ticerthan 3n， the stanard thckness of lpid blyer? ( du to aiti
4、oofprotein othe embraeand cbohydrte on thmembrane)3: Please dinte rnsion temeature o th idilayer(te temperature above hc he paracryslie soi cne to luid）1: If mbran ron has itsN-trmusesedto the outside te cell whle i C-terminus reides in thcytosoli cmpartent, is it a yeItransebran prot ? (yes).1: ow
5、n yo predict f a protenha trnsmrae oin? (hydrpayorhydrpathy index, hydropathy plot).1： Nam thetw cel srfac epor that HIVseo etr cells (CR5 and CD).17: For eNa+ +ATPase, how mnNa+ and + can t oveacrosthe memrne forthe ydrolis neATP （2 K+ in, 3Na+ot).18: what ivesF-typ TPase yntee ATP? （prtonoproton g
6、radients)19:Theceylhline reptorsa_-ged chan (ia)0: Thenerol Na+ chan is a _-tdcanel （ltage)II. nd H(3 poits)The iparallel rntation ofcmementarysrands in duplx NAasleatly deeridin 160 by Arthr orbrgy ere-neghbor analysis I his echqu, DA isyteize byDNA olymerse frm o (alpha32）-laled and threunlablld d
7、oxyucleosietihsptes. The resltng podcti hn droyzed by Dse that ceaves hsphoiestbon onth sieof alleyuclotids. For examle, i the labeed dTPreaton，pp*A + ppp+ ppG ppT - ppT*ApCpCp*ApGp*p*Tp - +CpT*+Ap+Cp+p*ApGp*+ATpTIf in he dATP abeedrecion the eativeioatvity ofTp*， Gp*， Cp adAp* is2, 03,0.4 d 0.1.In
8、th labeed dTPreacton,* rdioacivity will beA)1B) 0.2C) 0.D)4E)0In te labele dTreaction, Tp radioctit willb) 0.1G)0.2) 0.3)0.J) 0（B)( oint) A molecueof amloectin onsists of1000 gluose rsius anis branch er 25 esidue. How an ding edoeithave?) B) 1） 2D)40E）41F) 10(1 pint） ()A mior imae of frm DNA isA) Z-
9、DN) A-DAC) BDNAD） does noe innatr(1 pon) （A)Which of thefllown staemts is not tru?A) ll naua ccrrig DNAis in BformB) -proaby do not xist invivoC） Bthmajor grooe nd mnor goveof BDA re dee) BNA s rig handed, whie Z-DNA is left haned.(point) （A)ungfis hs 00,000，000kb (k=1000base pairs) for its haloid e
10、nome. h total leth of th DA is）34 mB) .4 mC) 1 mD) 17 mE） 1. m)34 m(3 oits?) (1. pot） A, D, EBoh tubes and Y cotai th meD n .1 M Clsolion. tube X or Nl is ed ad in tube Y soeethaisadde. hch ofte olowings （ar) corct?A)b Xw a hgher Tm hn Y） tube ill hgher Tm than X） tubandY willhave the same m）tue Xil
11、 av highr m han origial) tu will helor Tmrigial(1 ont） (）Whicofth llowingis N cort?A） lycoaiogynchains alinear) Glcoainolyca cains ar cidicC） Glycoaiolcans e oposd of repeating hride unitsD) Difeent glyoogycanshave he same disaccharid ni( poit) ()he s-cale tabe sgror ane sugaris a disachaidefA) glac
12、tseand gucose）2 gluoe uits) glos n rutoeD)aacose an frtose（1 pint) (A)Latoseisdaccaride ofA) glactoe nd gluoseB) 2 glcose unitC) guose and frucoeD）alatos andfuctose（1 point） （B)hoose he INCORRT atement. dideoxyseqencing or Sans mthod,A） the ewyynthsizeNA islabee while e telate inoB) th tempae s to be lable) a rme h teused) ddNTP are usedt
