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1、主要物质分子量195.2常见缓冲液的缓冲范围缓冲液名称及常用浓度缓冲 pH 范围MES (2 吗啉代乙磺酸)5.5-6.7Bis-Tris5.8-7.2HEPES 6.8-8.2 PIPES6.1-7.5MOPS6.5-7.9Tricine7.4-8.8TEA (三乙醇胺)7.4-8.3甘氨酸-盐酸缓冲液(0.05mol/L)2.2-5.0邻苯二甲酸-盐酸缓冲液( 0.05mol/L) 2.2-3.8 磷酸氢二钠-柠檬酸缓冲液2.2-8.0柠檬酸-氢氧化钠-盐酸缓冲液2.2-6.5柠檬酸-柠檬酸钠缓冲液(O.lmol/L) 3.0-6.6 乙酸-乙酸钠缓冲液(0.2mol/L)3.6-5.82
2、09.2238.3302.4209.3l79.2l49.2甘氨酸 Mr=75.07邻苯二甲酸氢钾Mr=204.23 磷酸氢二钠 Mr=l4l.98 柠檬酸 Mr=l92.06柠檬酸 Mr=l92.06 柠檬酸钠 Mr=257.96乙酸钠 Mr=81.76 乙酸 Mr=60.05邻苯二甲酸氢钾-氢氧化钠缓冲液4.l-5.9磷酸氢二钠-磷酸二氢钠缓冲液(0.2mol/L)5.8-8.0磷酸氢二钠-磷酸二氢钾缓冲液(1/15 molL) 4.92-8.18 磷酸二氢钾-氢氧化钠缓冲液(0.05mol/L)5.8-8.0氢钾 Mr=204.23巴比妥钠-盐酸缓冲液(18C)6.8-9.6Tris-盐酸
3、缓冲液(0.05mol/L 25 C)7.10-9.00甘氨酸-氢氧化钠缓冲液(0.05mol/L)8.6-10.6硼砂-氢氧化钠缓冲液(0.05mol/L) 9.3-10.1巴比妥钠Mr=206.18三羟甲基氨基甲烷(Tris)Mr=121.14 甘氨酸 Mr=75.07硼砂 Na2B4O7?10H2O Mr=381.43碳酸钠-碳酸氢钠缓冲液(0.1mol/L) 碳酸钠-氢氧化钠缓冲液(0.025mol/L) 磷酸氢二钠-氢氧化钠缓冲液 氯化钾-盐酸缓冲液(0.2mol/L) 氯化钾-氢氧化钠缓冲液(0.2mol/L) 参考下图：常见缓冲液的缓冲范围9.16-10.83 碳酸钠 Mr=28
4、6.2 碳酸氢钠 Mr=84.09.6-11.010.9-12.01.0-2.2氯化钾 Mr=74.5512.0-13.0氯化钾 Mr=74.55参考下图：常见缓冲液的缓冲范围S-SADAMOPSOMOKliTEE7fliS札1EPPS7RICINEWSBLVBLV常用缓冲液的配制方法1甘氨酸-盐酸缓冲液（0.05M）X ml 0.2M甘氨酸+Y ml 0.2M盐酸 再加水稀释至200mlpHX/ml Y/mlpHX/ml2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0Y/ml甘氨酸分子量=75.07 0
5、.2M甘氨酸溶液含15.01g/L 2.邻苯二甲酸-盐酸缓冲液（0.05M）X ml 0.2M邻苯二甲酸氢钾+Y ml 0.2M盐酸 再加水稀释至200mlpHX Y pH XY2.254.6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.032邻苯二甲酸氢钾分子量=2.4.23 0.2M邻苯二甲酸氢钾溶液含40.85g/L 3.磷酸氢二钠-柠檬酸缓冲液 pH 0.2M Na2HPO4/ml 0.1M 柠檬酸/ml pH 0.2M Na2HPO4/ml 0.1M 柠檬酸/ml2.2 0.4019.6
6、5.210.72 9.282.4 1.2418.76 5.4 11.15 8.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.7
7、31.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4 分子量= 141.98 0.2M 溶液含 28.40g/LNa2HPO4 2H2O 分子量= 178.05 0.2M 溶液含 35.61g/L C6H8O7 H2O 分子量=210.14 0.1M 溶液含 21.01g/L4.柠檬酸-氢氧化钠-盐酸缓冲液 pH钠离子浓度/M柠檬酸C6H8O7H2O/g氢氧化钠NaOH/g浓盐酸HCl/ml终体积/L2.20.2021084160103.10.2021083116103.30.2021083106104.30.20210834
8、5105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入1g酚，若最后pH有变化，再用少量50%氢氧化钠溶液或浓盐酸调 节，冰箱保存。5.柠檬酸-柠檬酸钠缓冲液（0.1M） pH 0.1M柠檬酸/ml 0.1M柠檬酸钠/ml pH 0.1M柠檬酸/ml 0.1M柠檬酸钠/ml3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.21
9、2.37.76.22.817.24.411.48.66.42.018.04.610.39.76.610418.64.89.210.8C6H8O7 H20 分子量=210.14 0.1M 溶液含 21.01g/LNa3C6H5O7 2H2O 分子量=294.12 0.1M 溶液含 29.41g/L6.乙酸-乙酸钠缓冲液（0.2M）pH 0.2M NaAc/ml 0.2M HAC/ml pH 0.2M NaAc/ml 0.2M HAC/ml3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.4
10、8.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc 分子量= 136.09 0.2M 溶液为 27.22g/L7.磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2M）pH 0.2M Na2HPO4/ml 0.2M NaH2PO4/ml pH 0.2MNa2HPO4/ml 0.2M NaH2PO4/ml5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.
11、577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HPO4 2H2O 分子量= 178.05 0.2M 溶液含 35.61g/LNa2HPO4 12H2O 分子量=358.22 0.2M 溶液含 71.64g/LNaH2PO4 H20 分子量= 138.01 0.2M 溶液含 27.6g/LNaH2PO4 2H2O 分子量= 156.03 0.2M 溶液含 31.21g/L
12、（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15M）pH 1/15M Na2HPO4/ml 1/15M KH2PO4/ml pH 1/15MNa2HPO4/ml 1/15M KH2PO4/ml4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.24 2.00 8.006.47 3.00 7.006.64 4.00 6.006.81 5.00 5.006.98 6.00 4.008.04 9.50 0.508.34 9.75 0.258.67 9.90 0.109.18 10.00 0Na2HPO4 2H2O 分子量= 178.05 1/15M 溶液含 35.61g/LKH2PO4 分子量= 136.09 1/15M 溶液含 9.078g/L 8.磷酸二氢钾-氢氧化钠缓冲液（0.05M）X ml 0.2M KH2PO4 +Y ml 0.2M NaOH 再加水稀释至 20ml pH(20C) X/ml Y/ml pH(20C) X/ml Y/ml5.8 50.3727.052.9636.050.5707.253.5006.2 50.8607.453.9506.4 51.2607.654.2806.6 51.7807.854.5206.8 52.3658.054.680 9.
