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1、溶液成分不同体积（ml）凝胶液中各成分所需体积（ml）5101520253040506% SDS-PAGE水2.65.37.910.613.215.921.226.530%丙烯酰胺溶液12345681010% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.000.0080.0120.0160.020.0240.0320.048% SDS-PAGE水2.34.66.99.311.513.918.523.230%丙烯酰胺溶液1.32.745.36.7810.713.31.5 mol/L Tris (pH
2、8.8)1.32.53.856.37.51012.510% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.000.0060.0090.0120.0150.0180.0240.03wo9T00乙 tcyoTOO80(X090(X0VOOOzOOOQ3IAI31SOVOGO宓()乙.()WOTOwoSOVOGO宓()乙.()WOTOwosas %otS7TOTSL&9S8Ts乙口(88Hd) sui i/|oiu Sl0乙9TaOT89z壑以翊骤蛔 0S9T乙切6,63906t97sovd-sas %a
3、WO9T00乙 tcyoTOO80(X090(X0VOOOZOOOasiAisiSOVO0宓()乙.()WOTOwoSOVOGO宓()乙.()WOTOwosas %otS7TOTSL&9S8Ts乙口(88Hd) sui i/|oiu SlETTOT爵Z9SE壑以翊骤蛔 086T6京6TT6,6676567SDVd-SQS %0T15% SDS-PAGE水1.12.33.44.65.76.99.211.530%丙烯酰胺溶液2.557.51012.51520251.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.510% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.000.0040.0060.0080.010.0120.0160.02
