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1、激光扫描共聚焦显微镜,在荧光显微镜成像的基础上加装了激光扫描装置，利用计算机进行图像处理，使用紫外光或激光激发荧光探针，从而得到细胞或组织内部微细结构的荧光图像， 观察细胞的形态变化或生理功能的改变。在药理学研究领域可以检测药物对细胞结构和生理功能的影响。Confocal laser scanning microscopy (CLSM or LSCM ) is a technique for obtaining high-resoluti on optical images with depth selectivity.The key feature of con focal microsco
2、py is its ability to acquire in-focus images from selected depths, a process known as optical sectioning. Images are acquired poin t-by-po int and recon structed with a computer, allow ing three-dime nsi onal recon struct ions of topologically-complex objects. CLSM is widely-used in numerous biological scienee disciplines, from cell biology and genetics to microbiology and developme ntal biology.
