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1、毕业设计(论文)中文题目：铀矿石冶金菌菌优势菌株的研究学生姓名：郭 勤学 号： 060552203指导教师：李 江专 业： 生物技术二零一零年 六 月 Graduation pproject(ppapperS)English title：Uranium mine metallurgy fungus supperiority strain researchName : Guo qinNumber : 06055203 Instructor : Li jiangMajor : Biotechnology June,，2010摘 要嗜酸性微生物是一类生活在极端酸性环境下的微生物,，已被广泛用于生物冶
2、金。对取自江西某铀矿堆场的嗜酸性微生物群落优势菌菌进行了分离和初步的研究分析与探讨。本文对采自江西某矿堆浸矿堆实验的六个样品：S1，S2，S3，S4，S5，S6，在以9K+S+Fe液体培养基对其混合菌群氧化铁的效率进行分析，结果发现S3，氧化铁的效率最佳。运用双层固体平板培养技术,，以琼脂糖为凝固剂,，以SJH为底层添加菌,，分离S3样品中的微生物,，得到86种嗜酸性氧化亚铁硫杆菌（暂定名为：A1，A2，A3，A4，A5，A6），1株氧化硫硫杆菌（暂定名为：B1），3株异养菌（暂定名为：C1，C2，C3）。微生物,根据不同各菌株在不同双层固体平板表面的菌落形态特征,， 显微结构镜下观察结果，并
3、结合16SrRNA基因扩增技术对所获得的菌种进行了初步鉴定菌种。群落中占优势的嗜酸性微生物为氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans,，简称A. f) 。对其中一株氧化亚铁铁硫杆菌（A6） 的培养温度、接种培养基初始PHppH值、及接种量对其生长状况的影响进行做了单因素探讨实验，。结果表明，A6氧化Fe2+最快的最适 培养温度为35，、最适初始pH值为2.3，。最合理佳的接种量为10% 。对初步研究了不同底物S、Na2S2O3和Na2SO3对其中一株氧化硫硫杆菌（B1）的不同底物对起其生长的影响做了初步研究，结果表明最适合B1的底物为Na2S2O3，其次为单
4、质S。研究结果简介关键词：嗜酸性微生物;；双层固体平板;；菌落形态；显微结构；分子鉴定；生长因子氧化亚铁铁杆菌；氧化亚铁硫杆菌I 东华理工大学毕业设计 Abstract AbstractThe acidophilia microorganism is a kind of life under the violent acidic environment microorganism, has widely used in the biological metallurgy. Has carried on the separation and the preliminary research an
5、d the discussion to the Jiangxi some uranium mine acidophilia microflora superiority fungus. this article to picks piles from Jiangxi some ore soaks experiments six samples: S1, S2, S3, S4, S5, S6 carries on the analysis in the 9K+S+Fe liquid media to its mix bacteria colony ferric oxides efficiency
6、, finally discovers S3, the ferric oxide efficiency is best. Utilizes the double-decked solid plating technique, take the agarose as the peptizer, take SJH as the first floor increase fungus, separates in the S3 sample the microorganism, obtains 6 kind of acidophilia ferrous oxide thiobacilli (A1, A
7、2, A3, A4, A5, A6), 1 sulphur oxide thiobacillus (B1), 3 heterotrophic bacterias (C1, C2, C3). According to the different fungus in the different dull surfaces colonial morphology characteristic, the microstructure, and unifies the 16SrRNA gene to increase the technical appraisal mold mushroom spawn
8、. Gets the advantage the acidophilia microorganism for the ferrous oxide thiobacillus (Acidithiobacillus ferrooxidans, is called A. f). To ferrous oxide thiobacillus (A6) the raise temperature, initial PH, the vaccination quantity has carried on the single factor discussion to its growth conditions
9、influence, finally indicated that A6 the optimum temperature for 35, most suitable pH is 2.3, the most reasonable vaccination quantity is 10%. To sulphur oxide thiobacillus (B1) the different substrate has done the preliminary study to its growths influence, finally indicated that to most suits B1 t
10、he substrate is the Na2S2O3 next for S. The acidophilia microorganism is a kind of life under the violent acidic environment microorganism, has widely used in the biological metallurgy. Has carried on the separation and the preliminary research and the discussion to the Jiangxi some uranium mine aci
11、dophilia microflora superiority fungus. Utilizes the double-decked solid plating technique, take the agarose as the peptizer, take SJH as the first floor increase fungus, separates in the sample the microorganism, obtains 8 kinds of acidophilia microorganism, according to the different fungus in the
12、 different double-decked solid dull surfaces colonial morphology characteristic, and unifies the 16SrRNA gene to increase the technical appraisal mold mushroom spawn. Gets the advantage the acidophilia microorganism for the ferrous oxide thiobacillus (Acidithiobacillus ferrooxidans, is called A. f).
13、 To a ferrous oxide ferrobacillus temperature, PH, the vaccination quantity has carried on the discussion to its growth conditions influence. To a sulphur oxide thiobacilluss different substrate to had the growth the influence to do the preliminary study.KeyWords:Acidophilia microorganism; Double-de
14、cked solid plate; 16s rDNA; Single factor Acidophilia microorganism; Double-decked solid plate; Colonial morphology; Microstructure; Molecular appraisal; Growth factorII东华理工大学毕业设计 目录 目 录摘 要I关键词：IAbstractIIKeyWords:II绪论1（1） 国内外研究现状1(2) 冶金微生物1(3)浸矿体系中的微生物2(4)生物冶金发展趋势及前景3(5) 冶金微生物的多样性3(6)环境微生物多样性的研究方法4
15、(7) 双层固体平板法4(8)本文的研究目的和意义41试验材料与仪器51.1 菌株来源：51.2 主要仪器51.3培养基51.3.1液体培养基51.3.2 固体培养基62 试验方法72.1菌株、菌液、菌体的保存方法72.1.1菌株的保存72.1.2菌液保藏的方法72.1.3菌体保藏的方法72.2 活性培养72.2.1富集方法72.2.2化学分析方法72.3 菌株的筛选和纯化82.3.1划线法：82.3.2 稀释涂布法82.3.3 极限稀释法82.4 菌株的鉴定82.4.1 菌株的鉴定82.4.2单菌落的富集培养92.4.2.1氧化亚铁硫杆菌属：92.4.2.2氧化硫硫杆菌属：92.4.2.3异养菌类：9
