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1、龙源期刊网 http:/胶原/氧化透明质酸复合水凝胶支架的制备 与表征作者：岩晓丽冯玉杰卢伟等来源：中国实用医药2011年第29期【摘要】目的制备复合水凝胶并初步考察其在软骨组织工程中的应用。方法用高碘酸钠 对透明质酸进行改性，与中性的胶原反应，37C下形成水凝胶。二甲氨基苯甲醛反应测透明质 酸含量并用TNBS法测胶原与氧化透明质酸的交联度。软骨细胞包埋于水凝胶中培养并观察其 生长状态。结果氧化透明质酸被有效固定于水凝胶中，胶原的氨基与氧化透明质酸的醛基反 应形成西夫碱结构，二者交联度随氧化透明质酸含量的增加而提高。软骨细胞在水凝胶中均匀 分布且活性良好，透明质酸的添加对细胞生长有一定促进作用
2、。结论胶原/氧化透明质酸复合 水凝胶仿生支架在软骨组织工程中有很大的应用前景。【关键词】胶原；氧化透明质酸；水凝胶；软骨细胞；软骨组织工程hydrOgel scaffo神and characterization of collagen oxygenated hyaluronic acid compositeBiomater槌擀 Sicuonunersitychengdu 6fd064,，ChinaEngineering Research Center for【Abstract ObjectiveA biomimetic hydrogel is prepared and investigated
3、 preliminarily in the application as cartilage tissue scaffold Methods Collagen and oxygenated hyaluronic acid (HA) which was prepared bysodiinn periodate wew mUed to form hyckogel under physiological CQndjiiQn Using the dimethylaminobenzadehyde reaction confirm the HA content and theNBS method Was applied to determine the crosslinking degree of composite hydrogel Chondrocytes of rabbits were embe鹳Re in the hydrogel, cultured in vitro and observed Results Oxygenated HA was fixed effectivelhydrogel and had no HA dissolving out of its Schiff s base formed between the amino of collagen
