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1、ITS1: 5 -CCGTAGGTGAACCTGCGG-3ITS4: 5 -TCCTCCGCTTATTGATATGC- Tm 55CNS17: CATGTCTAAGTTTAAGCAANS3: GCAAGTCTGGTGCCAGCAGCCNS4: CTTCCGTCAATTCCTTTAAGNS22: AATTAAGCAGACAAATCACTNS24: AAACCTTgTTACgACTTTTALROR: 5 -GTACCCGCTGAACTTAAGC-LR3: 5 -CCGTGTTTCAAGACGGGLR3R: 5 -GTCTTGAAACACGGA3)Positi on within S. cerevi
2、seae17S RNABMB-AGRATTACCGCGGCWGCTG580-558BMB-BCCGTCAATTCVTTTPAGTTT1146-1127BMB-CACGGGCGGTGTGTPC1638-1624BMB-BRCTTAAAGGAATTGACGGAA1130-1148BMB-CRGTACACACCGCCCGTCG1624-1640SR1RTACCTGGTTGATQCTGCCAGT21SR1ATTACCGCGGCTGCT578-564SR2CGGCCATGCACCACC1277-1263SR3GAAAGTTGATAGGGCT318-302SR4AAACCAACAAAATAGAA838-8
3、20SR5GTGCCCTTCCGTCAATT1146-1130SR6TGTTACGACTTTTACTT1760-1744SR6RAAGWAAAAGTCGTAACAAGG744-1763SR7GTTCAACTACGAGCTTTTTAu17-637SR7RAGTTAAAAAGCTCGTAGTTG3637-617SR8RGAACCAGGACTTTTACCTT732-749SR9RQAGAGGTGAAATTCT896-910SR10RTTTGACTCAACACGGG1181-1196NS1GTAGTCATATGCTTGTCTCNS2GGCTGCTGGCACCAGACTTGCNS3GCAAGTCTGGT
4、GCCAGCAG；CCNS4CTTCCGTCAATTCCTTTAAGi (similar to BMB-B)NS5AACTTAAAGGAATTGACGGAAGsimilar to BMB-BR)NS6GCATCACAGACCTGTTATT(3CCTCNS7GAGGCAATAACAGGTCTGTGATGCNS8TCCGCAGGTTCACCTACGGABMB = un iversal SSU primers developed by La ne et al., 1985SR = primers developed by Vilgalys labNS = primers described by W
5、hite et al., 1990Large sub un it RNA (25-28S) primer seque ncesNote: most molecular systematics studies only utilizethe first600-900 bases from the LSU gene, which in cludes three diverge ntdomains (D1, D2, D3) that are amongthe most variable regions withinthe en tire gene (much of the LSU is inv aria nt eve n across widelydiverge nt taxa). Most of the data in our Agaricales LSU
