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1、PCR 设计引物时酶切位点的保护碱基表 1关键词：不同内切酶对识别位点以外最少保护碱基数目的要求 不同内切酶对识别位点以外最少保护碱基数目的要求酶寡核苷酸序列切割率2 hr20 hrAcc IGGTCGACC00CGGTCGACCG00CCGGTCGACCGG00Afl IIICACATGTG00CCACATGTGG9090CCCACATGTGGG9090Asc IGGCGCGCC9090AGGCGCGCCT9090TTGGCGCGCCAA9090Ava ICCCCGGGG5090CCCCCGGGGG9090TCCCCCGGGGGA9090BamH ICGGATCCG1025CGGGATCCC
2、G9090CGCGGATCCGCG9090Bgl IICAGATCTG00GAAGATCTTC7590GGAAGATCTTCC2590BssH IIGGCGCGCC00AGGCGCGCCT00TTGGCGCGCCAA5090BstE IIGGGT(A/T)ACCC010BstX IAACTGCAGAACCAATGCATTGG00AAAACTGCAGCCAATGCATTGGAA2550CTGCAGAACCAATGCATTGG ATGCAT2590Cla ICATCGATG00GATCGATC00CCATCGATGG9090CCCATCGATGGG5050EcoR IGGAATTCC9090CGG
3、AATTCCG9090CCGGAATTCCGG9090Hae IIIGGGGCCCC9090AGCGGCCGCT9090TTGCGGCCGCAA9090Hind IIICAAGCTTG00CCAAGCTTGG00CCCAAGCTTGGG1075Kpn IGGGTACCC00GGGGTACCCC9090CGGGGTACCCCG9090Mlu IGACGCGTC00CGACGCGTCG2550Nco ICCCATGGG00CATGCCArGGCATG5075Nde ICCATATGG00CCCATATGGG00CGCCATATGGCG00GGGTTTCATATGAAACCC00GGAATTC CA
4、TATGGAATTCC7590GGGAATTCCATArGGAATTCCC7590Nhe IGGCTAGCC00CGGCTAGCCG1025CTAGCTAGCTAG1050大吕的日子酶切位点保护碱基表 2关键词：酶寡核苷酸序列切割率2 hr20 hrNot ITTGCGGCCGCAA00ATTT GCGGCCGCTTTA1010AAATATGCGGCCGCTATAAA1010ATAAGAATGCGGCCGCTAAACTAT2590AAGGAAAAAAGCGGCCGC AAAAGGAAAA2590Nsi ITGCATGCATGCA1090CCAATGCATTGGTTCTGCAGTT9090Pac
5、 ITTAATTAA00GTTAATTAAC025CCTTAATTAAGG090Pme IGTTTAAAC00GGTTTAAACC025GGGTTTAAACCC050AGCTTTGTTTAAACGGCGCGCCGG7590Pst IGCTGCAGC00TGCACTGCAGTGCA1010AACTGCAGAACCAATGCATTGG9090AAAACTGCAGCCAATGCATTGGAA9090CTGCAGAACCAATGCATTGGATGCAT00Pvu ICCGATCGG00ATCGATCGAT1025TCGCGATCGCGA010Sac ICGAGCTCG1010Sac IIGCCGCGG
6、C00TCCCCGCGGGGA5090Sal IGTCGACGTCAAAAGGCCATAGCGGCCGC00GCGTCGACGTCTTGGCCATAGCGGCCGCGG1050ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA1075Sea IGAGTACTC1025AAAAGTACTTTT7575Sma ICCCGGG010CCCCGGGG010CCCCCGGGGG1050TCCCCCGGGGGA9090Spe IGACTAGTC1090GGACTAGTCC1090CGGACTAGTCCG050CTAGACTAGTCTAG050Sph IGGCATGCC00CATGCATGCATG025ACATGCATGCATGT1050Stu IAAGGCCTT9090GAAGGCCTTC9090AAAAGGCCTTTT9090Xba ICTCTAGAG00GCTCTAGAGC9090TGCTCTAGAGCA7590CTAGTCTAGACTAG7590Xho ICCTCGAGG00CCCTCGAGGG1025CCGCTCGAGCGG1075Xma ICCCCGGGG00CCCCCGGGGG2575CCCCCCGGGGGG5090TCCCCCCGGGGGGA9090
