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6、三步：输入数据。第四步：选中AX、BY两列数据。第五步：点击菜单脸込山中的下拉菜单Fitting used）。Fit LirLH：=Lf1 (LastFile Edit View Plot Column Worksheetg i| 迎 Default： v|9|v B Z U x2 x2l c u 匚Ma them a ti csSigrial flocessingAbsorbancOriguF i 11 i ngIl ata M ani pul at i 皿ArL：alys i s Stati sties Image Tools Format Window HelpFit LinearFit
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9、ls Format Window Help猱彖!|BeSS7 ANOVA? Fitted Curves PlotVHILIU曰TUTh trrur huj. rc-toquart!Absorbance -0.7 0.834672.3 0.251 660.95377DFSum of SquaresMean SquareF Value ProbFAbsorbanceModel152.952.983.526320.00277Error31.90.63333Total 454.8AbsorbanceResidual vs. dependent PlotAbsorbance1JI |FitLinearl
10、 & FitLineaTCurvusl 阳启口也miAU : ON0 中丿 固：itLinearl Radian国链接厘 OriginPro 8 - d:My D.曲线拟合lipr=i回li翩卿ill第六步：双击Fitted Curves Plot那张拟合图。File E di t Vi ew Graph Data Analysi s Tools Format Window HelpTYTOokZmU新九片洋圍吕AIH酗诒s et Def,卅殳.久 0匚二| I像冒ts I需器L匚上出=gD ffl 睛分乞第| |so r b a n cbLinear F it of .a.bsorbance
11、田+*邑垸.mLI eq-ldsqv246Concentration/ .、z* 国至 I 画|： FitLine 1 RadianAU : ON Color Publication 1 ： Bookl jSheetl 中 ，W & H P 曹曲线拟合图-阿匚EditCopy page粘贴 Absorba neeLin ear Fit of Absorba nee12-QcnaDrosDA246Concen trati on第七步：对曲线横做表和纵坐标进行修改。双击横坐标Concentration或纵坐标Absorbance,对文字进行修改。新九片洋圍吕AIH酗诒s eFile E di t
12、Vi ew Graph Data Analysi s Tools Format Window Help11 |v|u习Bu / E城邮护C広辜+ I1!1!1* 田 fe転T / / 口(n-auueq.msqvV 0/ .、z* 国至 I 画藝 OriginPro 8 - d:My D.I： FitLine 1 RadianAU : ON Color Publi cati oil 1: Bookl She 胡目英固2 “ 議剔阴収For Help, preEditCopy page粘贴 Absorba neeConcen trati on( mg/ml)第八步：在曲线上取两个点。DataRemove Bad Data Points选择第曲线上得第一个点记录数据选择第二个点
