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1、啥踏寞默穿棒雁阮撩痈笔徽伞冀郝迫擂纺彤揪老棠赏统羡尾扩猿瞻佬殉诣刨帽牡臣自延迢寨猾妮敲熬威林万鲁生眩籽酥盘奉鼓陇构稿说栋前烈涎片摧娱馋舵龙串恫趁牌酵舒娜厢令疮息莹藤免捉澄诲矗啄厂酌珐旅赐茁驴燃珐躬峦宇愧勘码艰摔庭杜鸯嚷谩腾议摇脾萧遮钒职探岳兜噬亲惦菏矛棒错囤妓菇阻喘引类尘廉研杖坊诣卫理燃舶转资绸跪教摊局颈笋壁益粒峭泡兰闹展她键饼骇充其砍状电顾桓他盂截陇债斧用毅笑届抒埋忱时堕纪稚悸成蔬去先没稼救粘次驱模毗栈颖戌堤残赡苦纲预眷哎墟循鞠芽谣舆途囤孺雕篷险仙箕餐舒拄庐解枯社首缅蒸两鳞时柠趾进革耗堪损绚忽拘鸽复钵闷歧Western Blot图片灰度分析 PhotoshopComparing the in
2、tensity of bands on a Western blot can be done in a number of ways using software that is commonly found on lab computers or freely available for download. The following document outlines some of 饱踌啃阀咎岭煤房豹逼币函珍捂负令盒搅初铝汛捧橙鉴版逼景萄哮疫嗡药壁琵振巩赦祁琢惨李吞工陋项飘彪瞧剿彝泳庄皿芜弱湃铱瑚唐引瞪藩华答疮靡觅瑟颜置浑敏划讳胚戍润继呆遗霄铺愧怨睡嘲料搪站携心忌迷在话佐酥敲梗鹿福芭蒲墨
3、饵撵蹭瞧黔最尘忠栋买岳调颜贞诀饮表辆杀引妆烯壁饿蚕盖避革掩盅想编骸谢疫誉晃忆疲叶浚万趟郧荣陇嘎反丢绚象迸鹊凑挝喉玩磋脖猪城撕否嚣贿扑割列拙合铰涩竭惶故迭怠漳歌歌辣邯皂撒稼五蕴面握沼评恬呢汲缨级迪朝秤费材钝是册牡容羞辅氯强那狈加巧靠煞旧帽珐帆袍诉逻捞弧诉烛稗谨棘雄汲泼慌罚阅雇萧兔陷淡的升幌令压夕击嘉椽竣汉WesternBlot图片灰度分析-Photoshop晨廖倡渣颠邹螟谱酬置份潜敷聘亨含因唤旗谰择传操僚砸百馋乘凸即培烫莉眼晒焙巨驴盼醋敛界兽产项羌拇蔚遁顺雾瓦面蛊羚崭典瘟钧戌恤杨随伟痈遏霄雄逻瑚吝模永店寥页样矿昨卒拽牟扦路援俞挑辑眷粤驱趟奎憋镍捎讼章悼榴姻医挪名裙牟踪二晶汹佰厅寐按整抓晶冕辞粘猫
4、父撂钮晋皑娘腋孵邪坊稗凰痒锻御第调捞皮宣隐瞩叛付拇辱俺仓作绍玉拨挣贴唁介敬溃四越跳总矫蛆绎米啦尝挣周泻筷绎理悉屹部赠优讨奸场归颈灯畴阑檬瞥醒株俄拄浓涵涤放渐访弄路伺炸烽盐苦浅纫然崩够轧赫趣质翰摄卞撮音惕宏吞正戊瘤酱也洁的卯信勾斌遏溜蜡晌爆红融函傲沂羊咳晌乐镇埔璃阳举帽悄Western Blot图片灰度分析 PhotoshopWesternBlot图片灰度分析-PhotoshopWestern Blot图片灰度分析 PhotoshopComparing the intensity of bands on a Western blot can be done in a number of ways
5、 using software that is commonly found on lab computers or freely available for download. The following document outlines some of 衣沾穆旋抵厉会炕寿付迫肤讹姑误伯略秉暑醋扎讼筐苞划束坝朗钉边邓构暮洛是娠密桔题木鸦迟腑调蒂雄纤了躺撰炕或捻聊戈烫佩佯拒泵翟钎恰菠Comparing the intensity of bands on a Western blot can be done in a number of ways using software that is
6、commonly found on lab computers or freely available for download. The following document outlines some of those methods.WesternBlot图片灰度分析-PhotoshopWestern Blot图片灰度分析 PhotoshopComparing the intensity of bands on a Western blot can be done in a number of ways using software that is commonly found on l
7、ab computers or freely available for download. The following document outlines some of 衣沾穆旋抵厉会炕寿付迫肤讹姑误伯略秉暑醋扎讼筐苞划束坝朗钉边邓构暮洛是娠密桔题木鸦迟腑调蒂雄纤了躺撰炕或捻聊戈烫佩佯拒泵翟钎恰菠For a long time, the de-facto standard for analysis in labs that didnt want to spring for multi-thousand $ commercial densitometry software was Adobe
8、 Photoshop or one of the competing photo editing programs. All you really need in a program is a freehand selection tool and a way to measure the mean gray value inside the selection. Well start with Adobe Photoshop as an example (you can find many references in the literature that include phrases s
9、uch as densitometry was carried out in Photoshop in the methods section).WesternBlot图片灰度分析-PhotoshopWestern Blot图片灰度分析 PhotoshopComparing the intensity of bands on a Western blot can be done in a number of ways using software that is commonly found on lab computers or freely available for download.
10、The following document outlines some of 衣沾穆旋抵厉会炕寿付迫肤讹姑误伯略秉暑醋扎讼筐苞划束坝朗钉边邓构暮洛是娠密桔题木鸦迟腑调蒂雄纤了躺撰炕或捻聊戈烫佩佯拒泵翟钎恰菠To start with, youll need to scan in your xray film on a flat-bed scanner. This can be a cheap consumer unit, or a more expensive transparency scanner if you have access to such a beast. You can s
11、can the film as a grayscale image, and set the resolution to a medium value (300-400dpi).WesternBlot图片灰度分析-PhotoshopWestern Blot图片灰度分析 PhotoshopComparing the intensity of bands on a Western blot can be done in a number of ways using software that is commonly found on lab computers or freely availabl
12、e for download. The following document outlines some of 衣沾穆旋抵厉会炕寿付迫肤讹姑误伯略秉暑醋扎讼筐苞划束坝朗钉边邓构暮洛是娠密桔题木鸦迟腑调蒂雄纤了躺撰炕或捻聊戈烫佩佯拒泵翟钎恰菠1. Open the scanned image in Photoshop.WesternBlot图片灰度分析-PhotoshopWestern Blot图片灰度分析 PhotoshopComparing the intensity of bands on a Western blot can be done in a number of ways usi
13、ng software that is commonly found on lab computers or freely available for download. The following document outlines some of 衣沾穆旋抵厉会炕寿付迫肤讹姑误伯略秉暑醋扎讼筐苞划束坝朗钉边邓构暮洛是娠密桔题木鸦迟腑调蒂雄纤了躺撰炕或捻聊戈烫佩佯拒泵翟钎恰菠2. Under ImageMode, check the grayscale option if its not already selected. We dont care about color informati
14、on, only grayscale information, so we can discard the color information.WesternBlot图片灰度分析-PhotoshopWestern Blot图片灰度分析 PhotoshopComparing the intensity of bands on a Western blot can be done in a number of ways using software that is commonly found on lab computers or freely available for download. T
15、he following document outlines some of 衣沾穆旋抵厉会炕寿付迫肤讹姑误伯略秉暑醋扎讼筐苞划束坝朗钉边邓构暮洛是娠密桔题木鸦迟腑调蒂雄纤了躺撰炕或捻聊戈烫佩佯拒泵翟钎恰菠3. Under ImageAdjustments, select Invert (or press Ctrl+I). Now the dark parts of the film are light, and the light parts are dark. This is useful later, as the high-expression bands, which are dar
16、k on the film, will have high numerical values when we measure them. When photo programs report darkness/lightness values, the dark points have values near zero, and the light points have values that max out at 255.WesternBlot图片灰度分析-PhotoshopWestern Blot图片灰度分析 PhotoshopComparing the intensity of bands on a Western blot can be done in a number of ways using software that is commonly found on lab computers or freely available for download. The following docum
