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1、146种培养基成分1. Acetobacter Medium (醋酸菌培养基) (3q7Y&B Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g +4FNB CaCO3 20g Agar (琼脂) 15g eUV2& Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8 6r|E: 适用范围:恶臭醋酸杆菌混浊变种 :MfabpI $+Xa.H 9rQ?BBXP It is favorable to promote spore formation . E:e%J 适用范围:产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡
2、状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种(青虫菌)、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌(大肠杆菌)、铜绿假单胞菌(绿脓杆菌)、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌 r, ND y /8a( P-d0CH| vZA4(& oF=n!7ii 3. Azotobacter
3、Medium (固氮菌培养基) 8cLC3Fp KH2PO4 0.2g K2HPO4 0.8g ;dsCO MgSO4.7H2O 0.2g CaSO4.2H2O 0.1 A8 */$,9w a2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g / p$X Mannitol(甘露醇) 20g FeCl3 Tract(微量) h+mpq2y Distilled water (蒸馏水) 1000ml Agar (琼脂) 15g I9tuvcGqng Adjust (调) pH to 7.2 ;(Ej8)mM 适用范围:固氮菌、胶质芽孢杆菌 bVB *uBjN $
4、Pnq)5x :J/nGU/ z5dm|&Jf X-FXVcXx 4. Corn Meal Medium (玉米粉培养基) -X.o2X Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g IKSbF6 Glucose (葡萄糖) 1g Tap water (自来水) 1000ml 3Ug_mw6f Note:Boil the mixture in autoclave at 121 for 1 hr. distribute the medium into 1818 mm tubes , each contains 10 ml of the liquid , then
5、 autoclave at 121 for 1 hr . again (15磅蒸煮1小时,分装入1818毫米试管,每管深度达6厘米。15磅再次灭菌15小时。) jy5;/ gB,5qu :%jf;f ?!P,:/ ) 8 z 0Mh 5. Lactic-bacteria Medium I (乳酸菌培养基 I ) aY L&e Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g MFzCsF Glucose (葡萄糖) 10g KH2PO4 2g _X=2Z.|j1 Tomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml P
6、 Arw$Gr Distilled water (蒸馏水) 900ml pH 7.0 z0l) 5 适用范围:植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌 zfn2_Pm3# -ZG34wXOn gh!Qm6 9 /+NcVKm2 k_Sto 6. Lactic-bacteria Midium (乳酸菌培养基) V?dg- am?o , JDI58| 7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培养基) Z$9F-93x Peptone(蛋白胨)10g Yeast extract (酵母膏)5g enw Glucose (葡萄糖)
7、1g Distilled water (蒸馏水)1L Yq:W_=c 91DZhhMik &va4O/4MC .5/.TKj g V23#|B/7 8. Glycerol Agar (甘油琼脂) VFC xIO Peptone (蛋白胨)5g Beef extract (酵母膏)3g # $04% Agar (琼脂)15g pH 7.0-7.2 9.l(s An1L%/ 5 HHf;* V iY+J.0t# ?T_poK 9. Rhizobium medium (根瘤菌培养基)AS 9 EK/q_h2 Yeast eztract (酵母膏)1g Soil eztract (土壤浸提液) 200
8、ml ZxASrJp Mannitol (甘露醇)10g Agar (琼脂)15g GTmI QPr Distilled water (蒸馏水) 800ml pH 7.2 hjbDz Note:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121 for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minut
9、es at 121 , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小时,经滤纸过滤后加水补足到200毫升。) W f-(+8/F 适用范围:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、 HBBi)q 大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌 KTFzg VB=liFM| RHz0;| eVmJ7; AW np3 10. Mannitol Agar (甘露醇琼脂) c)VY_r- Yeast extract (酵母膏) 5g Pep
10、tone (蛋白胨) 3g cL*n Mannitol (甘露醇) 25g Agar (琼脂) 15g 6cci Distilled water (蒸馏水) 1000ml _ P=FRa7 p) +W wTgwn-!5 B rxOKp ,P-5s 11. Glucose Asparagine (葡萄糖、天门冬素琼脂) 2O cGc! Glucose (葡萄糖)10g Asparagine (天门冬素)0.5g N=X8; K2HPO4 0.5g Water (水) 1000ml SUj u pH 7.2-7.4 dmm 适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌) VYe6:U
11、!k k87OH +hF83bZ;a e.3-Z(e )/xb1k 12. Gauses Synthetic Agar (高氏合成一号琼脂) q+hdD|D NaCl 0.5g FeSO4 0.01g rMm9(Y5D Agar (琼脂)20g water (水) 1000ml ;%IEdSmD pH 7.2-7.4 y9l!xx 适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色链霉菌、烬灰链霉菌、天蓝色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉菌、灰色链霉菌、吸水链霉菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌 q.1ZdOa J 13. Wort Agar (麦芽汁琼脂) vp0)C Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word.Melt the agar by heating, then distribut