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1、各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCAL去做多重染色时是必不可少的参考资料。全部文件Excitation and Emission Wavelengths of FluorophoresTavis FluoroTable: Common fluorophores(from )FluorophoreAbsorption Emission Other info1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein504529
2、5-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668 Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)5786045675915-TAMR
3、A (5-Carboxytetramethylrhodamine)5485525425686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE520548430647449, 455471,474445630471,474526650520-650560-6807-Amino-4-methylcoumarin3517-Aminoactinomycin D (7-AAD)5467-Hydroxy-4-methylcoumarin3609-Amino-6-chloro-2-methoxyacridine412,430ABQ344Acid Fuchsin540A
4、CMA (9-Amino-6-chloro-2-methoxyacridine) 412,430Acridine Orange + DNA502Acridine Orange + RNA460Acridine Orange, both DNA & RNA440-480Acridine Red455-600Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460PhotoproteinAequorin (Photoprotein)466AFPs - AutoFluorescent Protein - (Quan
5、tumBiotechnologies) see sgGFP, sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495,492519,520Alexa Fluor 532531,532553,554Alexa Fluor 546556,557572,573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa Fluor 660668698Alexa Flu
6、or 680679702Alizarin Complexon530-560, 58580,624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345425347444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346442350445Aminomethylcoumarin (AMCA)345425347444Anilin Blue600Anthrocyl stearate360
7、-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG? CBQCA465560ATTO-TAG? FQ486591Auramine460550Aur
8、ophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381,382445, 447blue shifted GFP (Y66H)383448Blue Fluorescent ProteinBlue Fluorescent ProteinBFP/
9、GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505 Ratio Dye, Zn2+Blancophor FFGBlancophor SVBOBO?-1BOBO?-3Bodipy 492/515Bodipy 493/503Bodipy 500/510Bodipy 505/515Bodipy 530/550Bodipy 542/563Bodipy 558/5684704354816025155495155105475633903704625
10、70490533509502528543558Bodipy 564/570Bodipy 576/589Bodipy 581/591Bodipy 630/650-XBodipy 650/665-XBodipy 665/676570590592642665676564579584625647605504505505528542Bodipy Fl,505511, 513Bodipy FL ATP514Bodipy Fl-Ceramide511Bodipy R6G SE547Bodipy TMRBodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodi
11、py TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO?-1462481BO-PRO?-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529BTC-5N - atio Dye, Zn2+459/417517/532Calcein494517Ratio Dye Ca2+Ratio Dye, Zn2+Calcein Blue373440Calcium Crimson588, 589611,615Calcium Green501,506531Calci
12、um Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385,395437,405445440,Carboxy-X-rhodamine (5-ROX)576601Cascade BlueCascade YellowCatecholamineCCF2 (GeneBlazer)CFDACFP - Cyan Fluorescent ProteinCFP/YFP FRETChlorophyllChromomycin A377420398423399399550400552410470494520430,433474,475,CyanFluorescentProtei
