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1、1.核酸的换算：(1.1) 摩尔数与质量：1 mg 1,000bp DNA = 1.52 pmol1 mg pUC18/19 DNA (2,688bp) = 0.57 pmol1 mg pBR322 DNA (4,361bp) = 0.35 pmol1 mg SV40 DNA (5,243bp) = 0.29 pmol1 mg FX174 DNA (5,386bp) = 0.28 pmol1 mg M13mp18/19 DNA (7.250bp) = 0.21 pmol1 mg l phage DNA (48,502bp) = 0.03 pmol1 pmol 1,000bp DNA = 0.6
2、6 mg1 pmol pUC18/19 DNA (2,688bp) = 1.77 mg1 pmol pBR322 DNA (4,361bp) = 2.88 mg1 pmol SV40 DNA (5,243bp) = 3.46 mg1 pmol FX174 DNA (5,386bp) = 3.54 mg1 pmol M13mp18/19 DNA (7.250bp) = 4.78 mg1 pmol l phage DNA (48,502bp) = 32.01 mg(1.2) 光吸收值与浓度：1 OD260 dsDNA = 50 mg/ml = 0.15 mmol/L1 OD260 ssDNA =
3、33 mg/ml = 0.10 mmol/L1 OD260 ssRNA = 40 mg/ml = 0.12 mmol/L1 mmol/L dsDNA = 6.7 OD2601 mmol/L ssDNA = 10.0 OD2601 mmol/L ssRNA = 8.3 OD260(1.3) 分子量：1个脱氧核糖核酸碱基的平均分子量为333 Daltons1个核糖核酸碱基的平均分子量为340 Daltons(1.4) 核酸末端浓度：环状DNA：pmol ends = pmol DNA f number of cuts f 2线性DNA：pmol ends = pmol DNA f (number
4、of cuts f 2 + 2)1 mg 1,000bp DNA = 3.04 pmol ends1 mg pUC18/19 DNA (2,688bp) = 1.14 pmol ends1 mg pBR322 DNA (4,361bp) = 0.7 pmol ends1 mg SV40 DNA (5,243bp) = 0.58 pmol ends1 mg FX174 DNA (5,386bp) = 0.56 pmol ends 1 mg M13mp18/19 DNA (7.250bp) = 0.42 pmol ends1 mg l phage DNA (48,502bp) = 0.06 pmo
5、l ends2 核昔酸的换算：(2.1) 分子量：MW (Da) = 333 N (number of bases)(2.2) 浓度：C (mmol/L or pmol/ml) = OD260 / (0.01 f N)C (ng/ml) = OD260 f MW / (0.01 f N)MWmolecular weightNnumber of basesOD260absorbance at 260 nm(2.3) 双链DNA与寡核昔酸的熔点短于25bp的双链寡核昔酸：Tm = 2 (A + T) + 4 (G + C)长于25bp的双链寡核昔酸：Tm = 81.5 + 16.6 ( lgJ+ ) + 0.41 (%GC)-(600/N) - 0.63 (%Formamide)N引物的长度(以碱基数计算)J+单价阳离子浓度(2.4) DNA与表达蛋白之间分子量换算1 kb DNA = 333 amino acid = 3.7 f 104 Da10,000 Da Protein = 270 bp DNA30,000 Da Protein = 810 bp DNA50,000 Da Protein 2701 bp DNA100,000 Da Protein 27 kb DNA
