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1、 Center lab配制SDS聚丙烯酰胺凝胶电泳分离胶所用溶液成 分配制不同体积和浓度凝胶所需各成分的体积/ml5101520253040506%胶水2.65.37.910.613.215.921.226.530%丙烯酰胺混合液1.02.03.04.05.06.08.010.01.5M Tris(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.048%胶水2.34
2、.66.99.311.513.918.523.530%丙烯酰胺混合液1.32.74.05.36.78.010.713.31.5M Tris(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0030.0060.0090.0120.0150.0180.0240.0310%胶水1.94.05.97.99.911.915.919.830%丙烯酰胺混合液1.73.33.06.78.310.013.316.71.5M Tris(pH8.8)1.
3、32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0212%胶水1.63.34.96.68.29.913.216.530%丙烯酰胺混合液2.04.06.08.010.012.016.020.01.5M Tris(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20
4、.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0215%胶水1.12.33.44.65.76.99.211.530%丙烯酰胺混合液2.55.07.510.012.515.020.025.01.5M Tris(pH8.8)1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.02配制SDS聚丙烯酰胺凝胶电泳5%积层胶所用溶液成
5、 分配制不同体积和浓度凝胶所需各成分的体积/ml123456810水0.681.42.12.73.44.15.56.830%丙烯酰胺混合液0.170.330.50.670.831.01.31.71.0M Tris(pH6.8)0.130.250.380.50.630.751.01.2510%SDS0.010.020.030.040.050.060.080.110%过硫酸铵0.010.020.030.040.050.060.080.1TEMED0.0010.0020.0030.0040.0050.0060.0080.01SDS聚丙烯酰胺凝胶的有效分离范围丙烯酰胺浓度/%分离范围/kDa1510431212601020807.53694557212
