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1、.表3-1一些常用官能团的代表摩尔吸收系数值化合物类型发色团波长（nm）摩尔吸光系数胺NH2195苯C6H6184467002026900255170不饱和共轭（C=C）326035000（C=C）430052000（C=C）5330118000不饱和脂肪族C=C1908000（C=C）221023021000不饱和脂环族（C=C）223026030008000碘化物I260400叠氮C=N1905000二硫化物SS1945500255400砜SO2180复杂化合物C=CCC2916500C=CC=N22023000C=C=C=O21025010002000300350弱C=CNO222995
2、00腈CN160联苯C12N1024620000硫醇（巯基）SH1951400硫醚SO19446002151600硫酮C=S205强醚O1851000偶氮N=N285400325醛类CHO2101500280300118羧类COOH2002105070酮类C=O19510002702851530硝基NO2210强硝酸盐O NO227012溴化物Br280300肟NOH1905000亚砜S=O2101500亚硝基N=O302100亚硝酸盐O NO2202301000200030040010乙炔CC1751806000酯COOR20550表3-2 反相流动相组分的UV吸收值与波长的关系精品.在规定
3、波长处（nm）的吸收度（AU）200205210215220230240250260280溶剂 乙腈0.050.030.020.010.010.01 甲醇2.061.000.530.370.240.110.050.020.01除气后1.910.760.350.210.150.060.022.52.52.52.52.52.52.52.52.51.45酸和碱醋酸1%2.612.632.612.432.170.870.140.010.01盐酸6Mm（0.02%）0.110.020.01磷酸，0.1%0.01三氟醋酸0.1%水溶液1.200.780.540.340.200.060.020.010.1%
4、乙腈溶液0.290.330.370.380.370.250.120.040.010.01磷酸铵氢二铵，50Mm1.850.670.150.020.01三乙胺，1%2.332.422.502.452.371.960.500.120.040.01缓冲液和盐醋酸铵，10Mm1.880.940.530.290.150.020.01碳酸氢铵，10Mm0.410.100.010.01EDTA（乙二胺四乙酸），二钠，1 Mm0.110.070.060.040.030.030.020.020.020.02HEPESN-（2-羟乙基）哌嗪-N-2-乙烷磺酸，10Mm PH7.62.452.502.372.081
5、.500.290.030.01MES2-（N-吗啉代）乙基磺酸 ，10Mm PH6.02.422.381.890.900.450.060.01磷酸钾，二氢盐，10Mm0.030.01氢二盐，10Mm0.530.160.050.010.01精品.醋酸钠，10Mm1.850.960.520.300.150.030.01氯化钠，1m2.001.670.400.100.01枸橼酸钠，10Mm2.482.842.312.021.490.540.120.030.020.01甲酸钠，10Mm1.000.730.530.330.200.030.01磷酸钠，100 Mm，PH6.81.990.750.190.0
6、60.020.010.010.010.010.01Tris-盐酸，20 MmPH7.01.400.770.280.100.040.01PH8.01.801.901.110.430.130.01表面活性剂Brij35（23月桂醚），1%0.060.030.020.020.020.010.01CHAPS（3-3-胆胺丙基二甲基氨溶）-1-丙烷磺酸盐，0.1%2.402.321.480.800.400.080.040.020.020.01SDS（十二烷基硫酸钠），0.1%0.020.010.01Triton X-100（辛醇），0.1%2.482.502.432.422.372.370.500.25
7、0.671.42吐温20，0.1%0.210.140.110.100.090.060.050.040.040.03表3-3 在220nm时，低背景吸收（25 Mm磷酸钾（或钠）（p H5）25 Mm磷酸钠（或钾）（p H1.01.01.01.01.01.00.10乙醚1.01.00.460.271.080.100.05乙烷；0.540.200.070.030.020.010.00二氯乙烷1.01.01.01.40.090.110.05甲基-叔-丁醚1.00.690.540.450.260.110.05正丙醇1.00.650.350.150.070.030.01异丙醇1.00.440.200.110.050.030.02四氢呋喃1.01.00.700.500.300.160.09如有侵权请联系告知删除，感谢你们的配合！精品
