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1、pGL3-Enhancer VectorThe pGL3-Enhancer Vector contains an SV40 enhancer located downstream of luc+ and the poly(A) signal. This aids in the verification of functional promoter elements because the presence of an enhancer will often result in transcription of luc+ at higher levels.pGL3-Enhancer Vector
2、 Sequence Reference Points: Promoter (none)Multiple cloning region 158Luciferase gene (luc+) 881740 GLprimer2 binding site 89111 SV40 late poly(A) signal 17721993 Enhancer 20212249RVprimer4 binding site 23072326ColE1-derived plasmid replication origin 2564-lactamase gene (Ampr) 33294186 f1 origin 43184773upstream poly(A) signal 49045057 RVprimer3 binding site 50065025
