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1、生物信息学作业一、利用NCBI查找所需基因序列生科1201班 马子良 3702120113打开NCBI,点击GenBank选项8 Wt NC0I NMO2X*fXZUnCTTWGSTARItD Toonaii CWURCtlDNA&RNADomett SvudurvtGtntKt4MtOne 0tnORM 1 Miot MemeMrUiWftUVrowmStouect AAM)rtt Yaiom/ rraiiwtgi YuD0tffAWRfNceiaifomiAnoM ADMNC. 叶 *CeprnfMKUctemwt | PvivMy I Btowwtt I Aettty | ConM1点击
2、 exon 选项,查看 Homo sapiens parkinson protein 7 (PARK7)的外显子序列4411etQcrVtrrrwS01(toct(l(cctcccaccaaacMMC*4NI mmmbctcecweocctac*otcctMt(ctccCyeect4CI CUCMB0*|tCCaCM1catCCMMtYl ! MCCIMCCc cccmctM MtMCMfeciccMI ( claarc odciftCM ccccmooc ctcet(tcl eeetcrt Mcr rc c(MKctr tMeeeee accccctu( 4HIerCC tceetfa Kc
3、caceece ctececM teeecW1 tcBctce ccrctccete ccccccctc . pctctcc rctpcce 4MI tttacc cMtetc VnOVBrifUinikfU/IWWTttfTVlhoi iwrWEraw7i!Hynnwn7UOT ciccc ( ski oc3cecacea cruccrc c“xc”U21 0HCre cccccce ttMere(crtttt*etetccmcG2S1 ytcm CMmoort * cmccc tcrcac SMIc(cckm ctct(ccta Mdctcfcc ccct(cae tcccc(MOI vt
4、ttltaM ctctfltttf ctUttettt aMOMoift y*cuMSI MMittc cnaectcvt i(QCCt(cc Qctvcctc tftcttctoc fceCKM U2I Hattgtc ttevcM4 tCBtuceai UQ伏ec ：Wil arftteac* tee Ycttcxcee etcaeaeMtaeMl MCiMteM aMeeocecacumu a iMI /ecceu rrCMc eactaceeee kMU, Hy Meeer M2I eMCtart*catt( acuzy yMil cfoetttteccarcdctftatctttwc
5、tctcaccaattmmcqkmiMletaeaancuvwmmc(wMOtKMMCCUCMMCtCCMl 0CtCMtcmcttttttcrntctuttttuttttttttttMCC:W121 tvttee aMt(cttcetccewc tfcciMMC mcwwcm6181 Cf C QEecctcl241 tMtccEt cctcttttM t(tttttcctKCSI MTcetcta WWM “face cMMUteaM1 arctrttrtf coctfctca catttaacrc ttrttntrt cttcccatst ttccttcv M2I crcitttctca
6、c&ctctcf tccct( t(ccat(c :accettMi(tetttcc tctecoc cctgccs aeccttc cctcctcM Kyt Mleactf KCC wcctee qfuq u(f u tCMW* HOI 宅讥k aicticccca cCctctc caactccica eWMu tccacccfc Ml /wcee caw“ vtacMc ccttMccee “cxg Mtaccc 021 VrtttUt(CtCCCl CtCtrttCt :RUGil tc*ct Wotctct ccuccq.E eccictgccl cccuc ,Y Ml eccctc
7、M dcacteccccMca cccccttcu1 o2g cttgtaaaca tataacataa aaatggcttc caaaagagct ctggtcatcc tggctaaagg agcagaggaaatggagacggtcatccctgt agatgtcatg aggcgagctg ggctgatgccagattaaggtca ccgttgcagg cctggctgga aaagacccag tacagtgtag ccgtgatgtg gtcatttgtc ccttgaagat gcaaaaaaag ag4ggaccatat gatgtggtgg ttctaccagg aggt
8、aatctg ggcgcacaga atttatctga gtctgctgctg tgaaggagat actgaaggag caggaaaacc ggaagggcct gatagccgcc atctgtgcag6gt cctactgctc tgttggctca tgaaataggt tttggaagta aagttacaac acaccctctt gctaaagaca aaatgatgaa tggag7gtcattacac tttgcgcttgctactctgag aatcgtgtgg caattgttga agccctgaat ctgcgacgat cacttagagaaaaaagacgg
9、ggcaaggagg aacaggccgtcctgattctt acaagccgggtggcggctca agtgaaggcttaggaatcca ttctcactgtggcctgggac ccacttgttc gttcgctctacagcttcgagttaaagactaaacaaaacaggagcagcgaatggtaggtta atgtgttcag aagtcgctgt ccttactact tttgcggaag tatggaagtc acaactacac agagatttct cagcctacaaattgtgtcta tacatttcta agccttgttt gcagaataaa ca
10、gggcattt agcaaacta三、打开NCBI-BLASTPrimer-BLAST在线设讣引物软件，选择要设计的引物类型，Generic Primers,并将上述序列输入，下面以 Homo sapiens parkinson protein 7 (PARK7)3 号外显 子为例PrmerLAST N* mPfMMf BLAST: 9y Himeflo KRIvtmo PvvmtOand BlASYKPCR Temptale0文传文传Pnmer PeramoimUte my n lorwwd (5-X on pkn WaM)Um my wm rwww primw o Je H)proeva
11、eu# f of pdmrs to rvCurnPrtMftampetehMM(T-Exoovuron wloctkonE ion IttBcteiElOtlAMlCilA eANAM FCRvt gu 0H Z wcton .No prvfecvnce yFsi $ Esonirtto b i:bkumetnumMofMMiMmuttanoioeiormairw5*Of XMaofMMton .rRBf r rmM to wprwa 切 Katione 6 VWOM .Maa1000 I 1QOCOOO V四、点击Get Primers,得到引物，给出的三对引物任选一对即可，我们以第一对为例
12、二亠 RtnorLASTiRfMlt IwepUt.I I0J pHmee 9vtmr pn 4 IndHAbew: Mwq mMA (OrMtiwn tknX t Mem QrafiK vww of MimerMinQ CO - * Xeimrfe MaiiM pcHMf rpomtoquence 42力TemplJte itrndSUH38伽OCXwftW 輕 WFrw3r0 pmwfATT AAQQ TCACCOTTOOOOPM20120m ie90 00 000 00R*vrw prmwr FrouctAGGCTGGCATCAGGACAAAT2Mnus20MM90003 002 00PrimerRFtoduett onurgt小 D： ：J 乂二4 : PRtCXCTW xofDo yMem 25*n pcoten T (PARK门 twiyipt mrunt rrftftA五、打开UCSCImSilicoPCR,输入引物序列，点击submit,检验引物的唯一性UCSC
