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1、莱茵衣藻se两步法产氢用培养基的研究徐斐，何耀宗，何定兵，华泽钊 (上海理工大学医疗器械与食品学院，上海200093) 摘要：分析 T TAP(Tris-Acet-Phosphate)、seTA(se-Tris-Acet)、seP(se-Phosphate)、 seTH(se-Tris-HCl)和se这5种培养基对莱茵衣藻se生长产氢的煤响，并进行了培养基的含量 分析.结果发现，培养基的pH和氮索的含量是影响莱茵衣藻生长的重要因索;而培养基中醋 酸根的含量则冇助于提高厌氧产氢过稈中衣藻的氢酶活性,从而提高其产蛍量.综合考虑两 步法产氢对细胞生长周期和细胞密度的要求以及最终厌氧诱导获得的氢酶活性
2、，最终选择 seTA培养基作为莱茵衣藻比产氢用培养基.关键词：莱茵衣藻se;培养基；两步法产氢；氢酶Studies on the formulation of culture medium for production of hydrogen with Chlamydomonas reinhardtii-seXU Fei, HE Yao-zong, HE Ding-bing, HUA Ze-zhao(College of Medical Instrumentation and Foodstuff, University of Shanghai for Science and Technolog
3、y, Shanghai 200093, China)Abstract: Chlamydomonas reinhardtii-se with Ion ger growth cycle different from other wild strains used broadly before has been chosen in the two-stage photosynthesis and H2 production progress Using the length of cells5 growth cycle, the density of cells and the activity o
4、f induced hydrogenase as criteria, the optimal formulation of culture medium was obtained as seta (se-Tris-Acet) which contains acetic acid as C source, higher concentration of N and stronger pH buffer system based on the se formulation. The results also indicate that the Ph stability and the concen
5、tration of N exert a great in fluence on the growth of C reinhardtii-se and the acetic acid is the key substance for hydrogenase induction.Key words: Chlamydomonas reinhardtii-se; culture medium; two-stage photosynthesis and H2 production progress; hydrogenaseMe2SO浓度及预处理条件对组织工程化真皮活性的影响王欣I,华泽钊】，刘宝林I杨
6、光辉I崔磊I曹谊林2(上海理工大学医疗器械与食品学院，上海200093; 2.国家组织工稈研究与开发中心，上海 200235)摘要：以二甲基亚矶(MSO)为低温保护剂，在就Me2SO浓度、处理温度及时间对真皮成纤 维细胞的活性影响进行研究的基础上，研究了 MSO浓度对低温保存后组织工程化真皮活性 的影响.采用胎盼蓝拒染法和四卩坐盐(MTT)比色法检测细胞存活率.结果表明,MSO浓度、 预处理时间及温度对细胞及组织的活性有明显煤响.将添加低温保护剂后的细胞悬液在4C 下保持20min有利于保持较高的细胞活性;且当Me2SO的体积分数在10%20%时，可使低温 保存后的组织工程化真皮组织的细胞活性
7、保持在60%以上.关键词：二甲基亚矶；组织工程；真皮替代物；低温保存Effects of Me2SO and loading conditions on the cell viability of tissue-engineered dermal replacementWANG Xin1, HUA Ze-zhao1, LIU Bao-lin1, YANG Guang-hui2, CUI Lei2, CAO Yi-lin2 (1. College of Medical Instrumentation and Foodstuff, Univers让y of Shanghai for Scienee
8、 and Technology, Shanghai 200093, China; 2. National Tissue Engineering Research andDevelopment Center of China, Shanghai 200235, China)Abstract: In order to optimize the cryopreservation protocols of tissue engineered dermal equivalent, the effects of cryoprotectant concentration and the loading co
9、nditions on the cell viability of tissue-engineered dermal replacement were studied with dimethyl sulfoxide (Me2SO) as the cryoprotectant. The effects of Me2SO concentmtion and the exposure time/temperature to MSO on the cell viability of dermal fibroblasts were studied. Then experimewas conducted t
10、o investigate the effect of MSO concentration on the cell viability of freeze-thawed tissue-engineered dermal replacement, under the selected pretreatment cond让ions. Viabilities of the fibroblast and the dermal slices were determined by using the Trypan Blue staining and the modified MTT assay, resp
11、ectively. The results indicate that the cryoprotectant concentration (Me?SO) and the loading conditions (pretreatment time and temperature) have significant effects on the cell viability of the dermal fibroblast and the tissue-engineered dermal equivalent. In order to maintain a relatively higher ce
12、ll viability (360%) during the cryoprotectant loading stage, a combination of 4 C and 2() min is preferable. And if the Me2SO concentration ranged from 10% to 20%, the freeze-thawed dermal equivale nt can retain the cell viability over 60% Key words: Me2SO; tissue engineering; dermal equivalent; cry
13、opreservation饱和水溶液法制备磺胺甲噁哇的卩-环糊精包合物研究吴亚卿，李代禧，李保国，王娟(上海理工大学动力工稈学院，上海200093)摘要：采用饱和水溶液法制备了磺胺甲噁哇与卩环糊精的包合物.利用紫外光谱分析法验证 了包合物的形成.通过正交试验得到最佳制备工艺为包合原料摩尔比1:1、包合时间3h、包 合温度60C,其包合率可达86.03%遴热曲线分析得到包合比为1:1,并利用紫外分光光度法分 别在20C、30C、40C和50C下研究了磺胺甲噁啤的p环糊精包合物的稳定常数. 关键词:卩环糊精；磺胺甲噁卩坐；紫外光谱；稳定常数；包合物Preparation of inclusion
14、compound of P-cyclodextrin with sulfamethoxazoleWU Ya-qing, LI Dai-xi, LI Bao-guo, WANG Juan(College of Power Engineering, University of Shanghai for Scienee and Technology, Shanghai 200093, China)Abstract: The inclusion compound of |3-cyclodextrin (|3CD) with sulfamethoxazole (SMZ) was prepared wit
15、h saturated solvent method, and its formation was validated through ultraviolet-spectrophotometry (UV) analysis The results of orthogonal test show that the optimal parameters adopted in preparation are: 6()C, 2h mixing, 1:1 raw material molar ratio for P-CD/SM乙 and at that time the inclusion rate o
16、f 卩CD with SMZ will reach 86.03%. The molar ratio was determined as 1:1 through differenlial thermal analysis (DTA). The stability constants of inclusion compound in aqueous solution were also studied by using UV method at 20C, 30C, 40 C and 50C, respectively.Key words:卩-cyclodextrin; sulfamethoxazole; ultraviolet-spectrophotometry; stability constants; inclusion compound基于系统仿真的高温行车空调器性能分析与实验研究王涛I,卵志敏I刘颖I,王丹2
