第十章真核生物转录因子的DNA识别 图10-1 一段DNA大区域上的真核结构基因的转录单元 图10-2 转录激活的模型 图10-3 TATA Box与复合物晶体结构中的DNA顺序 图10-4 TATA Box 结合蛋 白（TBP）的结构 图10-5 TBP蛋白和含有TATA Box DNA片段复合物的结构 图10-6 DNA片段在TATA Box区域产生了严重的弯曲 图10-7 TBP 蛋白和TATA Box之间的专一性 相互作用 TBP-TFIIB-TATA Box ComplexNC2 binding precludes recruitment of TFIIB and TFIIA to a preformed TBP-DNA complex. Molecular surface representations of NC2 (blue mesh), TFIIB (solid gray), and TFIIA (red mesh) bound to TBP (green) and DNA (yellow/orange ribbons).TFIIA-TFIIB-TBP-DNA Complex(A) modification of K97C TBP with the affinity cleavage reagent IAAOP to produce K97C-OP and (B) the regions of the promoter that are expected to be cleaved, as indicated by the gray arrows.Molecular model of the quaternary K97C- TFIIB-TFIIA-TATA complex generated on the basis of ternary complex crystal structures.图10-8 Antennapedia homeodomain的结构 图10-9 Homeodomain的螺旋-转折-螺旋花样与 repressor的相同花样的比较 图10-10 DNA和一个单体的hemeodomain的相互作用 图10-11 四种不同转录因子的homeodomain的氨基酸顺序比较 图10-12 酵母转录因子 Mat 2-Mat a1与DNA结合 的异二体的结 构 图10-13 与DNA结合的Oct-1蛋白的POU区域 图10-14 POU区域的两个结构域一前一后地在相反的方向上结合在DNA双螺旋上 Structure of the ternary complex of the OCA-B1– 44 peptide, the Oct-1 POU domain, and an octamer element. Overview of the ternary complex showing the OCA-B peptide (purple), the Oct-1 POU domain (the POU homeodomain is in red; the POU specific domain is in yellow), and DNA containing the octamer sequence (position 5 is in green). About 20 amino acids of the OCA-B 1–44 peptide are well- ordered in the crystal.图10-15 DNA与POU区域间的顺序专一性相互作用 图10-16 p53蛋白的多肽链可分为三个具有不同功能的结构域 图10-17 p53寡聚化结构域 的四聚体结构 图10-18 p53蛋白DNA结合结构域的结构和折叠 图10-19 p53-DNA复合物中的DNA顺序 图10-20 DNA和p53蛋白的 顺序专一性相互作 用 图10-21 p53蛋白中易被突变的两个精氨酸残基与DNA和在L2和L3环间的作用 Comparison of the DNA-free form of the mouse p53 core domain with the DNA- bound and DNA-free forms of the human p53 core domain. The DNA-free p53 core domain is shown in green.ABDifferent Views of p53 Tetramers Bound to DNA. Four p53 core domains (designated as A, B, C, and D) shown in ribbon representation interact with two double-stranded DNA half-sites. The core tetramer is a dimer of dimers: A-B (in cyan) and C-D (in green). The four Zn ions are shown as magenta spheres.图10-22 p53蛋白中最易发生致肿瘤突变的三个结构区域 。