NEBufferActivityChartforRestrictionEnzymesNewEnglandBiolabsprovidesacolor-coded10XNEBufferwitheveryrestrictionendonucleasetoensure100%activity.Tohelpselectthebestconditionsfordoubledigests,thischartshowstheoptimal(supplied)NEBufferandapproximateactivityinthefourstandardNEBuffersforeachenzyme.IfBSAissuppliedwithanenzyme,itisincludedinallNEBufferactivityreactions.Inaddition,thetablealsoshowsrecommendedreactiontemperature,heatinactivationtemperatureandrecommendeddiluentbuffer.Pleaseclickhereforadditionalinformationabout:UniqueNEBufferActivity|DoubleDigestion|HeatInactivation|Activityat37°C|DiluentBuffers|Time-SaverEnzymes|HighFidelity(HF)RestrictionEnzymesThischarthasbeenupdatedtoreflectthechangeof54restrictionenzymestoNEBuffer4onNov.1,2008.ClickfortheChartLegend,IconDescriptionA|B|C|D|E|F|H|I|K|M|N|P|R|S|T|X|Z|EnzymeSuppliedNEBuffer%ActivityinNEBuffersHeatInactivation°CIncuba.Temp.°CDiluent1234AatllNEBuffer4050501006537AAcc65INEBuffer3+BSA1075100256537AAccINEBuffer45050101008037AAcilNEBuffer32550100506537AAcllNEBuffer4+BSA10100100No37BAcuINEBuffer4+SAM50100501006537BAfelNEBuffer42550251006537AAflllNEBuffer4+BSA50100251006537AAfllllNEBuffer3+BSA2575100508037BAgelNEBuffer11005010756537CAhdINEBuffer4+BSA257501006537AAlelNEBuffer41025101006537AAluINEBuffer4100100751006537BAlwINEBuffer450100101006537AAlwNINEBuffer450100501006537AApalNEBuffer4+BSA255001006525AApaLINEBuffer4+BSA10010010100No37AApeKINEBuffer3257510050No75BApolNEBuffer3+BSA1075100758050AAsciNEBuffer4010101006537AAselNEBuffer3NR75100ddNR6537BAsiSINEBuffer4+BSA501001001008037BAvalNEBuffer41075101008037AAvailNEBuffer45075101006537AAvrIINEBuffer4100100501008037BEnzymetopSuppliedNEBuffer%inB1%inB2%inB3%inB4HeatInactiva.Incuba.Temp.DiluentBaeGINEBuffer11007510506537ABaeINEBuffer4+BSA+SAM50100501006525ABamHINEBuffer3+BSA75100100dd100No37ABamHI-HFNEBuffer41005010100No37ABanINEBuffer4+BSA50100501006537ABanIINEBuffer410010501008037ABbsINEBuffer210010025756537BBbvCINEBuffer450100101008037ABbvINEBuffer210010025756537BBccINEBuffer1+BSA1005010506537ABceAINEBuffer3+BSA1001001001006537ABcgINEBuffer3+SAMNRNR100ddNR6537ABciVINEBuffer41005001006537CBclINEBuffer350100100dd75No50ABfaINEBuffer47550101008037BBfuAINEBuffer3075100106550BBfuCINEBuffer4+BSA10050251008037BBglINEBuffer35075100506537BBglIINEBuffer3107510010No37ABlpINEBuffer45010010100No37ABmgBINEBuffer3+BSA025100106537CBmrINEBuffer275100751006537BBmtINEBuffer22510025506537BBpmINEBuffer3+BSA751001001006537BBpulOINEBuffer31025100258037BBpuEINEBuffer4+SAM50100101006537BBsaAINEBuffer4100100100100No37CBsaBINEBuffer450100751008060BBsaHINEBuffer4+BSA501001001008037ABsalNEBuffer475751001006550BBsaJINEBuffer41001001001008060ABsaWINEBuffer4+BSA50100501008060ABsaXINEBuffer47510010100No37BBseRINEBuffer4100100751006537ABseYINEBuffer31050100506537BBsglNEBuffer4+SAM5075501006537BBsiEINEBuffer4+BSA50100101008060ABsiHKAINEBuffer4+BSA50100100100No65BBsiWINEBuffer3100100100258055ABsllNEBuffer31050100758055ABsmAINEBuffer4501001001008055BBsmBINEBuffer3751001001008055BBsmFINEBuffer4+BSA2550501008065ABsmINEBuffer475100751008065ABsoBINEBuffer21010010050No37ABsp1286INEBuffer4+BSA2525251006537ABspCNINEBuffer4+BSA+SAM10075101008025ABspDINEBuffer42575501006537ABspEINEBuffer301010008037BBspHINEBuffer410100501006537ABspMINEBuffer3NRNR100NR6537BBspQINEBuffer410501001008050BBsrBINEBuffer4501001001008037ABsrDINEBuffer2+BSA5010050758。