呼吸速率的测定呼吸速率的测定—广口瓶法广口瓶法 ]呼吸速率的测定广口瓶法 实验目的•呼吸速率是植物生命活动强弱的重要指标之一，常用于植物生理研究及农业生产实践等方面测定呼吸速率的方法虽然很多，但不外乎测定二氧化碳的释放量和氧气的吸收量两类方法本实验用广口瓶法测定植物的呼吸速率，并比较小麦吸胀的种子与幼苗的呼吸速率 ]呼吸速率的测定广口瓶法 实验原理•密闭容器中加入一定量碱液（一般用氢氧化钡），并悬挂植物材料，则植物材料呼吸放出的二氧化碳可为容器中氢氧化钡所吸收，然后用草酸滴定剩余的氢氧化钡，从空白和样品二者消耗的草酸溶液之差，可计算出呼吸释放的二氧化碳量，其反应式如下：Ba(OH)2+CO2→BaCO3↓+H2OBa(OH)2+H2C2O4→BaC2O4↓+2H2O]呼吸速率的测定广口瓶法 材料、仪器与试剂材料、仪器与试剂 •植物材料：吸胀小麦种、芽长0.5cm左右的小麦种子•仪器：广口瓶装置（见图2）、台秤、酸式滴定管、滴定管架一套•试剂：1/44 mol/L草酸溶液：准确称取重结晶H2C2O4 •2H2O 2.8651g，溶于蒸馏水中，定容至1000ml，每ml相当于1mg CO2。
•0.05mol/L 氢氧化钡溶液：Ba(OH)2 8.6g 或Ba(OH)2 •8H2O 15.78g溶于1000ml蒸馏水中如有浑浊待溶液澄清后使用•酚酞指示剂：称取1g酚酞，溶于100ml95%乙醇中贮于滴瓶中 ]呼吸速率的测定广口瓶法 实验步骤1. 取500ml广口瓶两只，装置如图所示瓶口加一三孔橡皮塞：一孔插入装有碱石灰的干燥管,以吸收进入瓶内空气中的二氧化碳；一孔插入温度计；第三孔供滴定用，实验时插一小橡皮塞，塞下悬挂一尼龙纱小筐供装植物材料用2. 空白滴定：拔出两广口瓶的小橡皮塞，向瓶中加入氢氧化钡溶液20ml，再塞进瓶塞充分摇动广口瓶几分钟，待瓶内二氧化碳全部被吸收后，拔出小橡皮塞，加入三滴酚酞，把酸滴定管插入孔中用草酸溶液进行滴定至红色刚刚消失为止，记下草酸溶液用量（ml）,即为空白滴定值]呼吸速率的测定广口瓶法 ]呼吸速率的测定广口瓶法 3. 材料滴定值的测定：倒出废液，先用自来水，再用煮沸并冷却过的蒸馏水洗净广口瓶，重加20ml氢氧化钡溶液于两广口瓶中取吸胀小麦种100粒同时称出重量装入小筐中，迅速挂于橡皮塞下，塞好塞子另一瓶取100粒芽长0.5cm左右的小麦种子，同时称出重量装入小筐中，操作同前。
开始记录时间，其间轻轻摇动数次有利于二氧化碳充分吸收，经30分钟后迅速打开瓶塞取出小筐，立即塞紧拔出小橡皮塞，加入三滴酚酞，用草酸滴定同前，记录草酸溶液用量，即为材料滴定值 4. 计算呼吸速率： ]呼吸速率的测定广口瓶法 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]呼吸速率的测定广口瓶法 。