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1、Thyroid cancer detection significance of Bcl-2Abstract Objective To investigate the expression of Bcl-2 in the incidence of thyroid cancer, development and prognosis.Methods immunohistochemical methods, mouse anti-human monoclonal antibodies to Bcl-2 mark 77 cases of thyroid cancer, 58 cases of thyr
2、oid adenoma and 40 cases of adjacent thyroid tissue and 28 cases of normal thyroid tissue. observe the different thyroid tissue expression of Bcl-2, and compare the positive rate of Bcl-2 positive results seen in thyroid cancer, thyroid adenoma and adjacent thyroid tissue. in thyroid carcinoma Bcl-2
3、 positive rate was 45.5%, higher than the thyroid adenoma (22.4%) (P = 0.006), adjacent thyroid tissue (17.5%) (P = 0.003) and normal thyroid Organizations (0) (P = 0.000). anaplastic thyroid carcinoma and follicular carcinoma of the Bcl-2 positive rate was significantly higher. presence lymph node
4、metastasis and clinical stage , cases, Bcl-2 positive rate significantly increased. Conclusions Bcl-2 overexpression may be associated with the occurrence of thyroid tumors, cancer tissue expression of Bcl-2 can be used as a reference indicator of thyroid cancer prognosis.Keywords: Thyroid Cancer, B
5、cl-2 Gene, Proto-Oncogenes, Gene Expression, ImmunohistochemistryThyroid cancer is a common endocrine cancer, women with a high incidence of clinical thyroid tumors in 5% to 10% of thyroid cancer 1 which differentiated thyroid cancer accounts for 90 percent, including papillary thyroid carcinoma and
6、 follicular thyroid carcinoma , undifferentiated carcinoma are rare, its high degree of malignancy and poor prognosis. domestic data show that the incidence of thyroid cancer in a significant increase in 2 In recent years, thyroid cancer-related genes and tumor marker studies progressed very quickly
7、. study confirmed 3,4, Bcl-2 gene is an apoptosis suppressor gene, first discovered in follicular B-cell lymphomas, and its product Bcl-2 protein inhibits apoptosis and prolong cell survival. The Studies using immunohistochemical techniques, by detecting Bcl-2 in thyroid cancer, thyroid adenoma, thy
8、roid cancer tissue and normal thyroid tissue levels of Bcl-2 study in thyroid cancer incidence in the development process role in order to reveal the Bcl-2 and thyroid cancer biological behavior and prognosis of relationships.1 Materials And Methods1.1 MaterialsAll specimens were obtained from Pekin
9、g University Shenzhen Hospital General Surgery from 2001 to 2009 cases of thyroid surgery between specimens, including: thyroid cancer, 77 cases (28 males and 8 females, mean 50 years), 58 cases of thyroid adenoma (male 22 cases, 36 females, mean 36 years), while taking 40 cases of adjacent thyroid
10、tissue (10 males and 30 females, with an average 37 years old, had no diagnosed by pathological examination of thyroid cancer invasion), 28 cases of normal thyroid tissue ( 9 males and females l9 cases, mean 35 years). pair of 77 cases of thyroid cancer histological classification according to WHO c
11、riteria: 55 cases of papillary carcinoma, follicular carcinoma l4 cases, undifferentiated carcinoma in 8 cases, according to standard clinical AJCC staging : I period of 30 cases, 23 cases, of 16 cases, of eight cases, of which 33 patients with lymph node metastasis, no lymph node metastasis in 44 c
12、ases. specimens were fixed in 10% formalin solution, embedded in paraffin buried, routine biopsy, 60 baking sheet 2 h, 37 overnight reserve.1.2 Immunohistochemistry ReagentsBcl-2 mouse anti-human monoclonal antibody, for the United States ZYMED companys products.SP-9000 Universal kit U.S. ZYMED Comp
13、any. Using known Bcl-2-positive tonsil as a positive control, with PBS instead of primary antibody as negative space controls.1.3 Test MethodsDewaxing hydration, 3% H2O2 treatment at room temperature 15 min, microwave antigen retrieval, normal goat serum incubated 30 min, dropping a resistance, 4 re
14、frigerator overnight incubation. Dropping biotinylated secondary antibody sign incubated at room temperature 30 min, dropping horseradish peroxidase-labeled streptavidin at room temperature 30 min, DAB color. dyed by hematoxylin, dehydrated, transparent, mounted.1.4 criteria Bcl-2 located in the cel
15、l membrane and cytoplasm to the cell membrane and cytoplasm appear yellow to dark yellow granules positive cells without Bcl-2 positive cells was negative, there are positive Bcl-2 positive cells in all specimens are two pathologists blinded read the piece, the results are inconsistent harmonization
16、 judge the final result.1.5 Statistical AnalysisAll data using SPSS 13.0 statistical package on a computer for statistical analysis, for .2 test to P 0.05 was considered statistically significant.2 Results2.1 Bcl-2 In Different Tissues Of The Thyroid Condition, Are Shown In Table 1.Bcl-2 light microscope showed positive reaction cell membrane and cytoplasm stained tan, brown granules seen as negative.Bcl-2 positive rate of
