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1、EnzymeAcc IBamH IBgl IICla IEcoR IEcoR VHinc IIHind IIIKpn INco INde INot IPst IPvu ISac ISal ISma ISpe ISph IXba IXho ISupplied Buffer10 M10 K10 H10 M10 H10 H10 M10 M10 L10 K+BSA10 H10 H+BSA +Triton10 H10 K+BSA10 L10 H10 T+BSA10 M10 H10 M+BSA10 HAcc I-0.5 K1 T1 M1 M0.5 K1 M1 M1 M1 M+BSA1 T0.5 K+BSA
2、1 M0.5 K1 M1.5 T1 T+BSA1 M0.5 K1 M1 MBamH I0.5 K-1 K1 K1 K1 K0.5 K1 K0.5 K1 K+BSA1 K0.5 K+BSA1 K1 K0.5 K1.5 T0.5 T+BSA1 K1 K0.5 K1 KBgl II1 T1 K-1 H1 H1 H2 K1 K1 T1 K+BSA1 H1 H+BSA1 H1 K0.5 K1 H1 T+BSA1 H1 H2 T1 HCla I1 M1 K1 H-1 H1 H1 M1 M1 M1 K+BSA1 H1 H+BSA1 H1 K1 M1 H1 T+BSA1 M1 H1 M1 HEcoR I1 M
3、1 K1 H1 H-1 H1 M1 M1 M1 K+BSA1 H1 H+BSA1 H1 K1 M1 H1 T+BSA1 H1 H1 M1 HEcoR V0.5 K1 K1 H1 H1 H-2 T1 K0.5 K1 K+BSA1 H1 H+BSA1 H1 K0.5 K1 H0.5 K+BSA1 H1 H2 T1 HHinc II1 M0.5 K2 K1 M1 M2 T-1 M1 M1 M+BSA1 T0.5 K+BSA1 M0.5 K1 M1.5 K1 T+BSA1 M2 T1 M1 MHind III1 M1 K1 K1 M1 M1 K1 M-1 M1 K+BSA1 K0.5 K+BSA1 M
4、1 K1 M1.5 K1 T+BSA1 M1 K1 M1 MKpn I1 M0.5 K1 T1 M1 M0.5 K1 M1 M-0.5 K+BSA1 T0.5 K+BSA1 M0.5 K1 L1.5 T+BSA1 T+BSA1 M0.5 K1 M1 MNco I1 M+BSA1 K+BSA1 K+BSA1 K+BSA1 K+BSA1 K+BSA1 M+BSA1 K+BSA0.5 K+BSA-1 K+BSA0.5 K+BSA1 K+BSA1 K+BSA0.5 K+BSA1.5 T+BSA1 T+BSA1 K+BSA1 K+BSA1 M+BSA1 K+BSANde I1 T1 K1 H1 H1 H
5、1 H1 T1 K1 T1 K+BSA-1 H+BSA1 H1 K1 T1 H1 T+BSA1 H1 H1 T1 HNot I0.5 K+BSA0.5 K+BSA1 H+BSA1 H+BSA1 H+BSA1 H+BSA0.5 K+BSA0.5 K+BSA0.5 K+BSA0.5 K+BSA1 H+BSA-1 H+BSA2 K+BSA0.5 K+BSA1 H+BSA0.5 T+BSA1 H+BSA1 H+BSA0.5 K+BSA1 H+BSAPst I1 M1 K1 H1 H1 H1 H1 M1 M1 M1 K+BSA1 H1 H+BSA-1 K1 M1 H0.5 T+BSA1 H1 H1 M1
6、 HPvu I0.5 K1 K1 K1 K1 K1 K0.5 K1 K0.5 K1 K+BSA1 K2 K+BSA1 K-0.5 K1.5 K+BSA1 K+BSA1 K1 K0.5 K1 KSac I1 M0.5 K0.5 K1 M1 M0.5 K1 M1 M1 L0.5 K+BSA1 T0.5 K+BSA1 M0.5 K-1.5 T+BSA1 T+BSA1 M0.5 K1 M1 MSal I1.5 T1.5 T1 H1 H1 H1 H1.5 K1.5 K1.5 T+BSA1.5 T+BSA1 H1 H+BSA1 H1.5 K+BSA1.5 T+BSA-1.5 T+BSA1 H1 H1.5
7、T1 HSma I1 T+BSA0.5 T+BSA1 T+BSA1 T+BSA1 T+BSA0.5 K+BSA1 T+BSA1 T+BSA1 T+BSA1 T+BSA1 T+BSA0.5 T+BSA0.5 T+BSA1 K+BSA1 T+BSA1.5 T+BSA-1 T+BSA0.5 T+BSA1 T+BSA1 T+BSASpe I1 M1 K1 H1 M1 H1 H1 M1 M1 M1 K+BSA1 H1 H+BSA1 H1 K1 M1 H1 T+BSA-1 H1 M1 HSph I0.5 K1 K1 H1 H1 H1 H2 T1 K0.5 K1 K+BSA1 H1 H+BSA1 H1 K0
8、.5 K1 H0.5 T+BSA1 H-2 T1 HXba I1 M0.5 K2 T1 M1 M2 T1 M1 M1 M1 M+BSA1 T0.5 K+BSA1 M0.5 K1 M1.5 T1 T+BSA1 M2 T-1 MXho I1 M1 K1 H1 H1 H1 H1 M1 M1 M1 K+BSA1 H1 H+BSA1 H1 K1 M1 H1 T+BSA1 H1 H1 M-Note: 1) It is confirmed that 10 units of each enzyme completely digest 1 g of DNA at 37C in one hour in 50 l
9、reaction mixture. 2) The concentration of Glycerol should be less than 10% to minimize star activity. 3) DNA may not be digested completely, when recognition sequences of two enzymes are close each other, or when DNA takes high-structure conformation.Relative activity for each reaction buffer: supplied, activity measured buffer: recommended bufferRestriction enzymeRelative activities (%)LMHKT+BSABasal*Aat II20202020100120Acc I20100
