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1、Poly-lysine-coated tissue culture surfaces1. To coat coverslips:Prepare a stock solution by dissolving 25 mg/ml polylysine in 4.73 ml water (both poly-L-lysine and poly-D-lysine are used to coat tissue culture surfaces; check specific protocol for choice of isomer) and filter sterilize through a 0.2
2、2-m filter. Store in 100-l aliquots at -20C. When ready to use, dilute one aliquot in 40 ml water to prepare 13 g/ml working solution. Sterilize coverslips by autoclaving prior to coating. Dip coverslips in the working solution, then incubate 15 min to several hours in a humidified 37C, 5% CO2 incub
3、ator. Allow surface to dry.2. To coat culture dishes: 多聚赖氨酸包被培养皿/板Prepare a stock solution by dissolving 100 mg poly-lysine in 100 ml water (both poly-L-lysine and poly-D-lysine are used to coat tissue culture surfaces; check specific protocol for choice of isomer) and filter sterilize through a 0.2
4、2-m filter.配制1mg/ml储存液Store in 5-ml aliquots at -20C. 分装保存在-20度When ready to use, dilute 1 part stock solution with 9 parts water to prepare 100 g/ml working solution. 使用前按1：10稀释成100g/ml工作浓度。用5-10ml一次性无菌注射器和0.22m滤器过滤除菌，备用。Fill tissue culture dishes or slide wells with the working solution and incuba
5、te 1 hr in a humidified 37C, 5% CO2 incubator, then remove solution by vacuum aspiration and allow surface to dry.加入培养皿或培养板中，使能完全覆盖皿底即可，37度培养箱放置1小时或置超净台内室温过夜。然后吸取液体，使表面晾干。Store coated tissue culture ware up to 3 months at 4C. 可以立即使用也可保存在4度3个月备用。Use diluted solutions only once, but unused diluted aliquots can be stored up to 3 months at 4C.加入细胞前用1XPBS 洗三遍，然后加入细胞和培养液。
