《选修三12基因工程的基本操作程序学案新人教版选修3》由会员分享,可在线阅读,更多相关《选修三12基因工程的基本操作程序学案新人教版选修3(5页珍藏版)》请在金锄头文库上搜索。
1、the partys fine style, carry forward the traditional Chinese virtues, practicing the Socialist core values, vigorously carry forward the spirit of Jiao Yulu, Hongqi Canal spirit and spirit of persistence, honesty in politics, strict statesman, stick to the spiritual heights of the Communists. Four t
2、o devotion, as, do play a role in qualifying. Official Communist duty. To fulfill the partys purpose, maintain for the people themselves, and dedication, devotion, maintain pioneer, pioneering and enterprising spirit, active at the well-off extra practice, make contributions. The incentive function
3、of party organizations at all levels should give full play to advanced models, establish a representative, advanced and typical of the times, and guide the broad masses of party members to emulate. In honor of the 95 anniversary of founding as an opportunity to award in recognition of a number of ou
4、tstanding party members advanced grass-roots party organizations, outstanding party workers, and put the pioneer trees. Third, the reform efforts for example, be sure to change into. Learning to teachEducation in order to solve the problem, if it does not solve the problem, it will form, goes throug
5、h the motions. To strengthen the consciousness of problems and insisted on problem-oriented, problem-solving learning education for traction, truly deep investigation into change, modification. A checked swing. Learning education programme for party-building in our city actually proposed to focus on
6、 solution of fuzzy ideals and beliefs waver, consciousness, purpose of the party weak sense, honesty and self-discipline awareness is not strong, depressed, six issues of ethical misconduct study contents specific programmes for all party members and leading cadres above the county level shall focus
7、 on further refinement to the problems. Specific to every party member and every cadre, also requires a combination of actual and controlled, focused and really put yourself in, people see things, see, with their own specific problems to learn Constitution Party rules, series, address, so precision
8、to find the problem, laying a solid foundation for further corrective action. B to make change. For check put of problem, through established rectification Taiwan account, and take special supervision, and on account PIN, approach, insisted do side learn side modified, and that know that modified, w
9、hile focused on put two learn a do learning education rectification with grasp party of mass line education practice activities and three strict three real topic education problem rectification combined up, consolidation expanded has made of rectification results, strongly corrected for officer not
10、for, and Chi and endless corrected four wind, and regulation masses side of abuses, ensure work advance more powerful, and problem solution more completely. Third, we must consolidate our achievements. For more public, some party members who focus on the outstanding issues, combining concentrated生物选
11、修3(二)专题1 基因工程12 基因工程的基本操作程序寄语;春光正好,最适奋马扬鞭;青春年少,更宜发愤图强。学习目标1、简述基因工程原理及基本操作程序。2、尝试设计某一转基因生物的研制过程。学习重点和难点1学习重点:基因工程基本操作程序的四个步骤。2、教学难点(1)从基因文库中获取目的基因。(2)利用PCR技术扩增目的基因。自主学习一、目的基因的获取:1目的基因:符合人们需要的,编码蛋白质的。2获取目的基因的方法 (1)从基因文库中获取目的基因基因文库:将含有某种生物不同基因的许多,导入受体菌的群体中,各个受体菌分别含有这种生物的不同基因,称为基因文库。基因组文库:含有一种生物的 基因种类cD
12、NA文库:含有一种生物的基因目的基因的获取根据基因的有关信息:基因的序列、基因在上的位置、基因的产物mRNA、基因的产物蛋白质等特性获取目的基因。(2)利用PCR技术扩增目的基因。PCR:是一项在生物体外特定DNA片段的核酸合成技术。条件:已知目的基因的序列。过程:目的基因DNA受热形成,与结合,然后在_的作用下进行延伸形成DNA。方式:指数扩增=2n(n为扩增循环的次数)(3)人工合成法:基因较小,核苷酸序列已知,可以人工合成。典例1 下列获取目的基因的方法中需要模板链的是( ) 从基因文库中获取目的基因 利用PCR技术扩增目的基因 反转录法 通过DNA合成仪利用化学方法人工合成A B C
13、D二、基因表达载体的构建1表达载体的组成:目的基因+标记基因等。2启动子:位于基因的,它是和结合的部位,驱动基因转录产生mRNA。3终止子:位于基因的,终止。4标记基因:受体细胞是否含有目的基因。5表达载体的功能:使目的基因在受体细胞中稳定存在,并且给下一代;同时使目的基因_和发挥作用。6不同的受体细胞及目的基因导入受体细胞的方法不同,基因表达载体的构建上也会有所差别。典例2科学家在培育抗虫棉时,经过了许多复杂的过程和不懈的努力,才获得成功。起初把苏云金芽孢杆菌的抗虫基因插入载体质粒中,然后导入棉花的受精卵中,结果抗虫基因在棉花体内没有表达。然后在插入抗虫基因的质粒中插入启动子(抗虫基因首端)
14、,导人棉花受精卵,长成的棉花植株还是没有抗虫能力。科学家又在有启动子、抗虫基因的质粒中插入终止子(抗虫基因末端),导入棉花受精卵,结果成长的植株,有了抗虫能力。由以上过程推知,作为目的基因的运载体应具备的结构是( ) A目的基因、启动子 B目的基因、终止子 C目的基因、启动子、终止子 D目的基因、启动子、终止子、标记基因三、将目的基因导入受体细胞(一)转化:目的基因进人受体细胞内,并在受体细胞内维持稳定和的过程。(二)方法1将目的基因导入植物细胞(1)农杆菌转化法农杆菌特点:易感染植物和裸子植物,对_植物没有感染力;Ti质粒的可转移至受体细胞,并整合到受体细胞的染色体上。转化:目的基因插人农杆
15、菌导入植物细胞目的基因整合到植物细胞染色体上目的基因的遗传特性得以稳定维持和表达。(2)基因枪法 基因枪法:是单子叶植物中常用的一种基因转化方法,但是成本较高。(3)花粉管通道法 花粉管通道法:这是我国科学家独创的一种方法,是一种十分简便经济的方法。(我的的转基因抗虫棉就是用此种方法获得的)2将目的基因导入动物细胞(1)方法:注射技术。(2)操作程序:目的基因表达载体取卵(受精卵) 显微注射注射了目的基因的受精卵移植到_性动物的_内发育新性状动物。3将目的基因导人微生物细胞(1)原核生物特点:繁殖快、多为单细胞、遗传物质相对较少等。(2)大肠杆菌最常用的转化方法是:处理细胞细胞表达载体与感受态细胞混合 _细胞吸收DNA分子。典例3采用基因工程的方法培育抗虫棉,下列导入目的基因的做法正确的是( )将毒素蛋白注