《mini-protean3详细操作手册》由会员分享，可在线阅读，更多相关《mini-protean3详细操作手册（26页珍藏版）》请在金锄头文库上搜索。
1、Mini-PROTEAN3 CellInstruction ManualCatalog Numbers165-3301165-3302For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723)Table of ContentsPageSection 1 General Information.11.1 Introduction .11.2 Components.21.3 Specifications .41.4 Chemical Compatibi
2、lity .41.5 Safety .5Section 2 Set Up and Basic Operation.52.1 Gel Cassette Preparation .52.2 Mini-PROTEAN 3 Cell Assembly and Sample Loading .82.3 Gel Electrophoresis .9Section 3 Separation Theory and Optimization.103.1 Introduction .103.2 SDS-PAGE (Laemmli) Buffer System.113.3 Native PAGE.12Section
3、 4 Reagent Preparation and Stock Solutions .134.1 Volumes Required Per Gel .134.2 SDS-PAGE (Laemmli) Buffer System.134.3 Discontinuous Native PAGE (Ornstein-Davis).154.4 Continuous Native PAGE .16Section 5 References .18Section 6 Maintenance .18Section 7 Troubleshooting.19Section 8 Product Informati
4、on and Accessories .21Section 9 Warranty Information.23Section 1General Information1.1 IntroductionThe Mini-PROTEAN 3 cell runs both hand cast gels and Ready Gel precast gelsinterchangeably. The Mini-PROTEAN 3 system includes a casting stand and glass plates withpermanently bonded gel spacers that s
5、implify hand casting and eliminate leaking duringcasting. The cell can run one or two gels, and the mini tank is compatible with other Bio-Radelectrode modules for tank blotting, 2-D electrophoresis, and electro-elution.Fig. 1. Mini-PROTEAN 3 system components.121.2 ComponentsTo get the best perform
6、ance from your Mini-PROTEAN 3 cell, familiarize yourself withthe components by assembling and disassembling the cell before using it (refer toFigures 1 and 2).Spacer Plate The Spacer Plate is the taller glass plate with gel spacerspermanently bonded. Spacer Plates are available in 0.5 mm, 0.75 mm,1.
7、0 mm, and 1.5 mm thicknesses, which are marked directly oneach Spacer Plate.Short Plate The Short Plate is the shorter, flat glass plate that combines with theSpacer Plate to form the gel cassette sandwich.Casting Frame The Casting Frame, when placed on the benchtop, evenly alignsand secures the Spa
8、cer Plate and the Short Plate together to form thegel cassette sandwich prior to casting.Gel Cassette Assembly One Casting Frame, a Spacer Plate, and a Short Plate form one GelCassette Assembly.Casting Stand The Casting Stand secures the Gel Cassette Assembly during gelcasting. It contains pressure
9、levers that seal the Gel CassetteAssembly against the casting gaskets.Gel Cassette Sandwich A Spacer Plate and Short Plate with polymerized gel form a GelCassette Sandwich after casting.Combs A selection of molded combs is available.Buffer Dam The molded, one-piece buffer dam is used when running only onegel.Electrode Assembly The Electrode Assembly holds the Gel Cassette Sandwich. It housesthe sealing gasket, the upper and lower electrodes and theconnecting banana plugs. The anode (lower electrode) banana plugis identified with a red marker and the cathode (upper e
