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1、Agricultural ScienceTechnology，2011，12(2)：237240 Copyright201 1，Information Institute of HAASAll rights reserved Plant Protection PreIl mi nary Study on Garl i c Root Exudates I nfl uences to the Growth of Pomegranate Wi lt Pathogen (Ceratocystis fimbriata)and Bacillus subtilis TANG Dongsheng ，WANG
2、Bin，MAO Zhong-shun，HE Xia-hong Key Laboratory for Agrobiodiversity and Pest control of Ministry of Education，Yunnan Agricultural University，Kunming 650201 Abstract f ohiectivesThis paper aims to explore the possibility to intercrop garlic with pomegranate tree to controI pomegranate wiltI Meth- ods
3、1 Root exudates of garlic is cultivated in 15 concentration of MS solution and distilled water is examined in fab to test their effect to growth of mycelia of pomegranate wilt pathogen(Ceratocystis fimbriata)and multiplication of Bacillus s“6 I Results I The result shows that garlic root exudates wh
4、atever cultivated in MS solution or distilled water could not inhibit or promote mycelia growth of Cfimbria招Howevergarlic root ex udates cultivated in both methods effectively promote multiplication of Bsubtilisj Conclusions I It is suggested that intercropping garlic with pomegranate tree by combin
5、ing application Bsubtilis could be a promising way to prevent pomegranate wilt spread in practice Key words Garlic；Pomegranate wilt：Ceratocystis fimbriata；Bacus subtilis；Root exudates Punica granatum Linnis one of the traditional character Istic fruit treesMengzi County in Yunnan Province has been o
6、ne of the six Iargest Punica granatum Linnproduction areas and has a plantation history of more than 800 yearsPunica granatum Linnis the advantaqe0us production for Iocal farm ers to earn more moneyThe plantation scale is the top one in ChinaThereforeMengzi is named as”Chinese Punica granatum LinnCo
7、unty“l1 JAlong with the advance of Mengzi pomegranate industry，the plantation area is expanding and the age of the tree is increasingThe disease disaster of pomegranate is more and more seriousAmong廿1emthe withered pomegranate caused by ceratocystis fimb ate is the most serious onePomegranate wither
8、ed disease is one kinds endangered and serious disastrous disease，which could re suited into died pomegranate in more than two weeks Thus it might result in the destruction of the entire pomegranate gar- den and seriously influence the development of pomegranate IndustryThe disease develops rapidly
9、since 1 999 and results into serious Iosses to pomegranate production L2 JSince re ports on pomegranate withered diseasethough some investi- gations on disease occurrence regularity and pathogen identifi cation have been done there are no reports on the effec tive methods of field diseaseprevention
10、-sj As advantaaeOus agriculturaI products of high economic value of highgrade fruit and earning foreign exchange through exportnon-chemicaI method is applied to controI pomegran ate withered disease and decrease the application of agricu1 turaI diseasesand plays an important role in enhancing pomegr
11、anate quailty and economic outcomeIn recent years the antibacterial action of biocontrol bacteria such as Bacillus subtilis shows a good prevention outcome jn many vegetation tungal pathogens such as Fusarum oxysponum tcurneri- n“m and coon withered disease【 Howeverafter plant ing bioprevention bact
12、eria into solIIt can no effectively es- tablish and proliferate because of influence frOm root envi Received：December 3，201 0 Accepted：January 30，201 1 SuppoSed by National”973“Project(201 1 CB1 00400) Corresponding authorE-mail：eastuptang1 26com rOnment multi-biology and nonbiological factorsshowin
13、g in- consistency of field prevention outcome and indoor biologicaI measurement resultsTherefore，to find effective disease pre vention technology measures and to strengthen survive ratio of biological bacteria in solI have been important study sub iectAs one kind of common root bacteria，the growth a
14、nd proliferation of Bacillus subtilis often rely on the cOrrespOndina root system exudates in the surroundings released incessantly by depending vegetation root system。Under careful agricul- tural managementweeds in fruit garden are totally erased 1ts vegetation biodiversity is extremely Iow and fru
15、it solIis ba sically in naked stateBesides of pomegranatenearly no rOots exudates of other vegetation could be applied as solf bio- controI bacteriaThereforestudy on the Importance of recov- ering pomegranate vegetation biodiversity on pomegranate withered disease control is very necessary The impor
16、tance of garlic root system exudates on with ered pomegranate disease and the growth of Bacillus subtilis are tested。The possibility of using garlic to enhance pome granate vegetation biodiversity and to improve withered pome- granate disease controlled by Bacus subtilis is discussed Materials and Methods Test vegetation Garlic stem is bought frOm market Tested bacteria Cfimbriata and BsubtilisBSY2 are supp
