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1、为了适应公司新战略的发展，保障停车场安保新项目的正常、顺利开展，特制定安保从业人员的业务技能及个人素质的培训计划生物实验报告英文格式生物实验报告班级姓名组员生物系实验报告姓名班级学号实验日期XX科目普生实验实验名称RegenerationinPlanarian合作者指导教师成绩LAB8:RegenerationinPlanarianIntroduction:Planarian(flatwormDugesiajaponica)isoneofaseriesofanimalspeciesthathavetheabilitytore-growtheirbodypartswhendamaged.When
2、itsbodyiselongated,itissoft,leaf-shapedandciliated.Ithasspade-shapedheadwithtwoeyesandtentacleswhilethetailispointed.Itsmouthisevenontheventral.Whenitcomestoitsinnerstructures,ithasnobodycavity(coelom),soitspharynxmayendsinanintestine.Itusuallyis3to15mmlong,butitcanalsogrowtomorethan30cm.Itmovesbysw
3、imminglikeslugs,tendingtoseekfoodwhichincludesprotozoans,tinysnailsandwormsatnightascarnivorouscreature.Planariansarehermaphroditesandtheirreproductiveorgansstarttodevelopinearlyautumn,buttheycannotinbreed.Mostoftheseindividualsareabletofullydevelop,whilesomemaybeabnormal.Andbecauseofplanariansremar
4、kableabilitytoregeneratelostparts,theyareoftenusedinbiologyexperiments.Inthisexperiment,wewillhaveanopportunitytoobserveandrecognizesomeofthebasicfeaturesofplanarians.Wewillbetaughttousescientificapproachtohypothesizehowplanariansrespondtofragmentation.Theplanarianwillbeworkedasmodel.Materialsandmet
5、hods:Materials:Stereoscope(Nikon),Handlenses,Petridishwithfrozenwater,Knife,Tweezers,Droppers,Filterpapers,Planarians.Methods:Part:Observetheplanarian.Usehandlensestohaveacloseobservationonplanarian,andrecordwhatareseenintableT-1.Identifythefollowingfeaturesandmakenotesaccordingly:color,shape,moveme
6、nt,length,symmetry,visiblebodyparts,responsestolight,foodandflippingtheanimalupsidedown.Summarizewhatwehavefoundout,andtrytofindconnectionsbetweendifferentaspectsofplanariansbiology.Part:ExploringregenerationinPlanariansbycuttingaPlanarian.Labelapetridishwithmarkpen.PlaceaPlanarianonwetfilterpaperpl
7、acedontopofbedoffrozenwaterinapetridish,sothatitcanberelativelystill.BisectthePlanarianintotwopieces,likeUseadroppertodislodgethePlanarianoffthefilterpaperandintothelabeledpetridish.Fillthepetridishabouthalffullwithwater.Part:ObserveregenerationswhichcomefromtrisectionofPlanarianovertime,basedonthes
8、amplesprovidedbyteachers.CutaPlanarianhowwewantto,placethepartsintothelabeledpetridish,fillitabouthalffullwithwater.ObserveatdifferenttimePoints(2hours,1day,2days,3days,7days),andmakenotesaboutthechangesincolorandtheprogressionoftheregeneratingpartsintableT-2.Results：Part:TheshapeofPlanarianbenefits
9、toitslivinginwater,suchasaspade-shapedhead.Andwhenfeeditmeat,itwillapproachtotheobjectiveslowly;thenifnothingthreatenit,itwillreachoutitsmouthparttothefood.Whenitmoves,itwilltryitsbesttoavoidlightsource.Part:(BasedontheseriesofFigure2)Afterbeingcuttotwoparts,theycanbothstillmovesinpetridish.Relative
10、ly,thepartofheadismoreactivethanthepartoftail.ThefrozenwaterdoesmakethePlanarianremainrelativelystill,sothatitiseasiertocut.ThedevelopmentofthesetwopartswillbegiveninTableT-2.Part:IntheteacherssamplesaretrisectionofPlanarian,thetimeis,respectively,whentheyareobservedonBesidesthesamplefromPart,inours
11、amples,thePlanarianiscutfromheadtotail(bisect),likeTableT-2(AsshownintheseriesofFigure3),IguessitmaybecomeaPlanarianwhittwoheads.Discussions:Question1:whycanPlanarianregeneratebutwecannot?Whydoyouthinksomeanimalscanregeneratebutotherscannot?Planarianisalowformoflife,thestructureisrelativelysimple.So
12、itisabletoregeneratebycelldifferentiation.Butcellsofhumankindhavebeenhighlydifferentiated,thestructureisverycomplex,regeneratingmaymakedaughtercellswrongwhichleadstodeath.Themorehighlythecreaturedifferentiates,theharderittendstobeabletoregenerate.Forexample,highformoflifessomaticcellsarealmostallhig
13、hlydifferentiated,therearehardlyothercellstomakeupthelostpartoftheorgan.Question2:IsaPlanarianimmortal?Doesitage?Fromtheviewofthisspecie,itisimmortal.Butitcanusebothsexualandasexualreproductiontoreproduce,whenitusesexualway,ithasagrowthprocess,soitisnotimmortalanditages.Question3:HowmightPlanarianbe
14、usefulformedicalresearch?Itmayhelptostudythemechanismofimmortal,whichmayhelpbiologiststoinventthemedicinewhichmakeshumankindneverbeold.References:Contributionstatements:*isresponsiblefortheobservationofPlanarian.ThecutofmaterialsbelongstoLiu*and*.Figures:areall10Figure1-1Figure1-2Figure2-1Petridishw
15、ithtwoPlanarians.2Planariansareeating.AftercuttingasPart.Figure2-2Figure3-1Figure3-2ThetailpartofPart,active.Themiddlepartofteachersssample.Thepartwithmoreorgans.(when)(ripcutting)Figure3-3Figure2-3Figure2-4ThepartwithfeworgansThegenerationoftailpartThegenerationofheadpartin(ripcutting)inPart.Part.Figure3-4Figure3-5Figure3-6ThegenerationofthepartwithThegenerationofthepartTheheadpartofteachersorgans(ripcutting)withlittleorgans(ripcutting)(when)EffectofTypeofSugaronGlycolysisandFermentationinYeastIntroduction
