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1、学报JournalofChinaPharmaceuticalUniversity 2009, 40( 2): 157- 1602690FA1T李 特,李运曼* ,刘丽芳,王宇新,李丰文,朱兴祥(S0Sv3,2210009)K 1 :对威灵仙总皂苷(TSRC)抗类风湿关节炎的作用机制进行研究bZE:利用刀豆蛋白 A ( ConA)诱导小鼠 T淋巴细胞增殖模型;利用大鼠 IL-1BaIL-2aTNF-A定量酶联检测试剂盒测定原发性佐剂性关节炎大鼠血清中 IL-1BaIL-2aTNF-A的含量;利用氢氧化钾-甲醇溶液法测定原发性佐剂性关节炎大鼠肿胀足前列腺素 E2 ( PEG2 )的含量bT: TS
2、RC能抑制 ConA诱导小鼠 T淋巴细胞的增殖;降低大鼠佐剂性关节炎细胞因子 ( IL-1BaIL-2aTNF-A)的含量;降低佐剂性关节炎大鼠炎性肿胀足 PGE2的含量b: TSRC可能是通过抑制 T淋巴细胞的过度增殖a抑制细胞因子和 PEG2来发挥抗类风湿性关节炎作用b1oM 威灵仙总皂苷;类风湿性关节炎; T淋巴细胞;白介素;肿瘤坏死因子-A;前列腺素 E2ms| R965 DSM A cI| 1000- 5048( 2009) 02- 0157- 04Mechanism of totalsaponinsofRadixClematidison rheumatoid arthritisLI
3、Te, LIYun-man* , LIU L-i fang,WANG Xin-yu, LIFeng-wen, ZHU Xing-xiangDepartment of Physiology, ChinaPharmaceutical University,N anjing 210009, ChinaAbstract Aim:To study theactionmechanism of totalsaponinis ofRadix Clematidis (TSRC) on rheumatoid ar-thritis (RA). Methods: The proliferation ofConA-in
4、duced T lymphocytemodelwas observed to find outwhetherTSRC had some effect on the proliferation ofT lymphocytes; The serum concentrations of interleukin-1 ( IL-1B),interleukin-2 ( IL-2) andTNF-A in adjuvan-t induced primary arthritis rats weremeasured by the enzyme-linkedimmunoadsorbent assay (ELISA
5、) kits1And the concentrations of PGE2 in the swollen feet of adjuvan-t inducedprimary arthritis ratsweremeasured by themethod of potassium hydroxide-methanol1Results: Itwas found thatTSRC inhibited theproliferation ofConA-inducedT lymphocytes inmice and decreased the concentrationsofboththe cell fac
6、tor and PGE2 in the adjuvan-t induced primary arthritis rats1Conclusion: The ant-iRA effect ofTSRCmight attributed to the inhibition on the proliferation ofT lymphocytes inmiceand theattenuations in the concen-tration of both cell factors and PGE2 in the adjuvan-t induced primary arthritis rats1Keyw
7、ords total saponins ofRadixC lematidis; rheumatoid arthritis; T lymphocytes; interleukin; TNF-A; PGE2A1( rheumatoid arthritis, RA)B&f%hbShq0132% 0134%,?hBM=qr20%,YrB3b.dA10d8F0aTFA0a/Fbd8F0a8F0SO4E%h,QbyN,srQlA10-1Z_b26(Clematis chinensis Osbeck1).SML262,9,A,Yerb51A=e,8!,u$,y%1b26c0aa?#ZnaCaaFeaN ia
8、Mg,0,R0,-?C20 2b26T.dA0,5PM,0r1,yN,YV2690( total samponins ofRadixC lema-tidis, TSRC)lT%a?Q4157*l 2008-11-11 *YT Te:l 025- 85325383 E-mai:l yucaoren sina. con学 报 Journal of ChinaPharmaceutical University401vb%y0( IL-1BaIL-2aTNF-A)#?Q41vn=PEG2YFAT),#54Gb1 111 药品与试剂2690( TSRC,90c63%,S0Sv004i4);A( ConA
9、,SSigma); RPM I1640!x(SGibco);ldb(13);MTT(SSigma); IL-1BaIL-2aTNF-A_k4(ZS/LK,|sY0506139a0506140a0506141)b4(30zK2304,|: 021205)bZY(20,|: 030228);fQ4( Freundcs complete adjuvan,t FCA,?10 mL/,SSigma,|033k8933)bk4(grsBb112 仪 器Napco-6100CO2!Q(S);EL301Strip readerSN(SB io-Tek);R06119,s;9(ZSNK)b113 动 物rBSD
10、v,b),8( 180 ? 20)g;l,b),8( 20? 2) g,28L4,3V|: ()2005-0001b2 Z E211 TSRC 对小鼠脾淋巴细胞细胞毒性实验 31421- 1422)|l,g,PF,8u50Q,200DCVr,FTris-NH4Cl 20 mL5min,( 1 000 r/min) 5 minb,FRPM I1640!,( 1 000 r/min) 5 min,2Q,KF15%ldbRPM I1640!A2 3 mL%,%65 106,i%iqb96d)%!dF%A100LL, TSRCaa4F( 50, 5, 015 Lg/mL)aF( 011 Lg/mL)b
11、F(50LL/d,F!4db37 ea5% CO2!Q!72 h,!-4 h,dFMTTA(5 g/L)20 LLb!F%A,!8 h,SN570 nmo)|l(A),TA(VUb212 TSRC对由 ConA诱导小鼠 T淋巴细胞增殖的影响31426- 1428)|l,!%A,15%ldbRPM I1640!A65 106,96d)%!dF%A100LL#ConA 50 LL(i5 mg/L), TSRCaa4F( 50, 5, 015 Lg/mL)aF(011Lg/mL)bF(d50 LL,F!4db37 ea5% CO2!Q!72 h,!-4 h,dFMTTA( 5 g/L) 10LLb!F%A,!8 h,SN570 nmo)|dA
