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1、 EZChrom Elite Client/Server User Training CourseEZChrom Elite Client/Server Users Training Course1st Day - AMOverviewConfigurationInstrumentsMethodsEZChrom Elite Client/Server Users Training CourseEZServersClientsInstrumentsOffline ProcessingEZChrom Elite Client/Server Users Training CourseBuilding
2、sBuildings LabsLabs InstrumentsInstruments ViewsViewsLogging inInstrument accessSave passwordSelect domainSelect projectMain MenuExplorer viewToolsLocationsInstrumentsConfigurationLets the data system know what instruments, detectors, and accessories are connected to it.Re-configure if you add or re
3、move a detector, or when you add new instruments.Configuration New Instruments Detectors Autosamplers External events Optional programsGeneric Configuration Detectors Events Analysis OptionsDetector Configuration Name detector Detector model Acquisition sourceEvents Configuration Event name Source S
4、etupConfiguring Instrument ControlInstrument ConfigurationConfiguring 5890 Communication Address EPC channels DigitalConfiguring 6890Configuring 6890 Communication Address Detector types Digital5890 Autosampler Config Set communication Select installed opts Dual tower6890 Autosampler Config Set comm
5、unication Select installed opts Dual towerBreak TimeBreak Time!InstrumentsEach instrument attached to the system has its own icon, and its own instrument window where methods and sequences are developed.Instruments can be opened on- line (where data acquisition can take place), or they can be opened
6、 off-line (for off-line method development).Instruments Create/edit methods Create/edit sequences Run samples Run sequences View data ReprocessInstrument Window Menus Tool bars Spreadsheets Channel select Chromatogram (trace)Instrument WindowMulti-ChannelMulti-ChannelCaution!Chromatogram Window Trac
7、es Appearances Operations UtilitiesAxis setup Graph title Get current limits General optionsAppearance Color scheme Graph Trace ApplyAnnotations Per trace annotation Select annotations Other selections Apply to allSpreadsheets Peak/group (calibration) tables SequencesMethods Acquisition Integration
8、Calibration External events Export Custom reportsMethodsInstrument Setup Channel on Sampling rate Run time DelayMethodsTriggerControl run startMethodsExport Enable Select parameters Field separator Options ODBCMethodsColumn/Performance Column info Descriptive info Calculation selectionMethodsCustom
9、Parameters Per-Peak or System Wide Create Additional Parameters Print, Export, AnnotateMethodsFiles User programs Subtract baseline fileMethodsAdvanced ReportsDo Exercises Configure Instruments Open chromatograms Chromatogram appearance Chromatogram annotation1st Day - PM1st Day - PMMethods - contin
10、uedIntegrationCalibrationGraphical Programming ToolbarGraphical Integration Click event Click location on chromatogram AnalyzeManual Integration Fixes Saved with data file One-time integration fixesIntegration Integration events table View events Edit eventsThreshold Distinguish start/stop of peak f
11、rom noise and driftPeak Width Initial value use narrowest peak New peak width when peak width doubles Set graphicallyDo Exercises Graphic integration Timed events table Manual integration fixesCalibrationRelates component amount to detector response. Why Calibrate? Non-linear detectors Different res
12、ponses from different compounds Instrument changes over time Operator errorCalibration4 Basic Steps1. Run known sample2. Define calibration peaks3. Enter level amounts4. Run standardsCalibrationRun known sampleDefine PeaksPeak TableEach row represents a componentExpected RT and window automatically
13、enteredPeak Table Properties Click to remove unwanted columns from peak tableDo Exercises Run single sample Graphically define peaks Complete peak table Save methodCalibration CurvesSingle levelMulti-levelResults not valid until calibration curve is completeMulti-level calibration Multiple standard
14、samples Each level represents a different standard amount Each level becomes a point on the calibration curveMulti-level calibration How curve is fit to the data Fit types Goodness of fitExternal Standard Calibration Assumes exact reproduction in injection amount No internal standard correction appl
15、iedReview Calibration Peak list Spreadsheet ReplicatesReview CalibrationOverlay fitsStatisticsDo Exercises Review calibration curve Overlay fit types Determine best fit Remove outliers2nd Day - AM2nd Day AM Sequence Generation and Acquisition Method Custom ReportingSingle Run Acquisition Run Info Amount Values Calibrate DescriptionSequences List of samples to be run Designates calibration andunknown samples Spreadsheet formatSequence Wizard Create new acquisition sequences Create new sequences for reprocessingSequence WizardSelect MethodSequence WizardUnknownsSequence WizardCalibrationSeq
