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1、噬菌体侵染细菌的实验Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.通过前面的学习,我们得知DNA是遗传物质 ,而蛋白质不是,但是许多人对艾弗里的实 验提出了质疑?在当时的技术下,艾弗里实
2、验中提取的 DNA,纯度最高时也还有0.02%的蛋白质。科学家设想:最好把DNA与蛋白质区分开,直 接地、单独地观察DNA和蛋白质的作用。Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.更
3、具说服力的更具说服力的 “ “噬菌体侵染细菌的实验噬菌体侵染细菌的实验” ”1952年赫尔希（A.Hershey)和蔡斯（M.Chase)Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.大
4、肠杆菌大肠杆菌T T2 2噬菌体噬菌体Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.噬菌体的遗传物质噬菌体的遗传物质 是蛋白质还是是蛋白质还是DNADNA 呢？呢？Evaluation
5、only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.大肠杆菌大肠杆菌T T2 2噬菌体噬菌体C C、H H、OO、N N、P PC C、H H、OO、N N、S S（标记（标记3232p p）（标记（标记3535s
6、 s ）T2噬菌体化学组分中,60%是蛋白质,40%是DNA,其中硫仅存 在于蛋白质分子中,99%的磷都存在于分子中Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.噬菌体侵染细菌的实验噬菌
7、体侵染细菌的实验 在分别含有放射性同位素在分别含有放射性同位素3232p p 和和3535s s的培养基中培养细菌。的培养基中培养细菌。 分别用上述细菌培养分别用上述细菌培养T T2 2噬菌体，制噬菌体，制 备含备含3232p p 的噬菌体的噬菌体和和含含3535s s的噬菌体的噬菌体 。 分别用含分别用含3232p p 的噬菌体的噬菌体和和含含3535s s的噬的噬 菌体菌体去感染去感染未被标记的大肠杆菌。未被标记的大肠杆菌。Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Prof
8、ile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.用用含含3535s s的噬菌体的噬菌体去感染去感染未被标记未被标记 的大肠杆菌。的大肠杆菌。上清液放射性很高，沉淀物放射性很低。上清液放射性很高，沉淀物放射性很低。Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5
9、 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.用含用含3232p p 的噬菌体的噬菌体去感染去感染未被未被 标记的大肠杆菌。标记的大肠杆菌。上清液放射性很低，沉淀物放射性很高。上清液放射性很低，沉淀物放射性很高。Evaluation only.Evaluation only. Created with Aspose.Slides
10、 for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.用35S标记的一组主要在上清夜中检测到放射性，而 用32P标记的一组，却在试管的沉淀物中检测到放射 性。想一想，这一结论说明了什么？Evaluation only.Evaluation only. Created with Aspose.Slides for
11、 .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.细菌烈解后，释放出大量的T2噬菌体中，可以检测到32P标 记的DNA，但却检测不到35S标记的蛋白质，这又说明了什 么呢？Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5
12、 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.噬菌体侵染细菌的噬菌体侵染细菌的过程过程Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides
13、 for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.DNADNA是噬菌体的遗传物质。是噬菌体的遗传物质。结论：结论：Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.
14、Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.DNADNA是主要的遗传物质是主要的遗传物质 DNADNA是噬菌体的遗传物质。是噬菌体的遗传物质。 RNARNA是是烟草花叶病毒等的遗传物质。烟草花叶病毒等的遗传物质。 原核生物真核生物原核生物真核生物DNADNA和和RNARNA均有，均有， DNADNA是遗传物质。是遗传物质。 绝大多数生物绝大多数生物DNADNA是遗传物质。是遗传物质。少数少数 生物只有生物只有RNARNA一种核酸，一种核酸，RNARNA是遗是遗 传物质。传物质。Evaluation
15、only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.谢谢大家Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd.
