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1、AbstractIIHe-Ne 激光对 9 号淡水小球藻生物量及总脂 含量积累的影响摘 要本文通过研究不同光照时间(0min、1min、2min、4min、8min、12min、16min)的 He-Ne 激光照射对 9 号淡水小球藻（Chlorella pyrenoidosa）产生诱变，探究其对 9 号小球藻生物量以及藻细胞总脂含量的影响。在此阶段中定期取样测量其吸光度值以换算细胞密度，最后对藻细胞进行总脂含量的测定。实验结果表明，1min、12min 和 16min 光照时间长度的 He-Ne 激光对 9 号小球藻的生物量积累有极其显著的促进作用。1min、2min、4min 和 8min
2、 光照时间长度的 He-Ne 激光对 9 号小球藻总脂含量的积累有显著促进作用。综合考虑，可对 1min 的诱导组筛选高产油藻株，为后期的遗传变异稳定性检测打下基础，以期为生物柴油规模化生产的研究提供优质藻种。关键词：9 号淡水小球藻，生物量，生物柴油，He-Ne 激光，总脂量。AbstractIIIAbstractThis thesis have studied the biologic effects including biomass and lipid content of the Chlorella pyrenoidosa which is irradiated with He-Ne
3、 laser of different gradient(0min/1min/2min/4min/8min/12min/16min). In this stage,the number of Chlorella cells in nutrient solution was count with the plate method using blood cell counts.A standard curve about the relevance between the biomas and the OD value of Chlorella cells would be drawn.And
4、the nutrient solution would be sampled periodic to measure the OD value in order to work out the biomas. At last the lipid content of the Chlorella would be measured. The results show that the growth of the Chlorella obviously was rising along with the time of laser irradiation of 1min/12min/16min a
5、nd the lipid content of Chlorella was rising along with the time of laser irradiation of 1min/2min/4min/8min.In conclusion the time of laser irradiation of 1min could be chosen as the high-level lipid microalgae for the research of stability about the genetic mutation and Large-scale production of b
6、iodiesel.Keywords：Chlorella pyrenoidosa，biomas，biodiesel，He-Ne laser，lipid contentAbstractIV目录III目录摘 要 .IABSTRACT.II第一章 引言.- 1 -1.1 课题的背景和意义.- 1 -1.2 9 号小球藻.- 2 -1.3 He-Ne 激光器.- 3 -1.3.1 He-Ne 激光器的相关参数 .- 3 -1.3.2 He-Ne 激光器的结构 .- 3 -1.3.3 He-Ne 激光器的工作原理 .- 4 -第二章 材料与方法.- 8 -2.1 9 号小球藻的培养.- 8 -2.1.1
7、藻种.- 8 -2.1.2 藻种培养.- 8 -2.2 实验材料 .- 8 -2.2.1 药品与试剂.- 8 -2.2.2 仪器.- 9 -2.3 实验方法 .- 9 -2.3.1 9 号小球藻的诱导.- 9 -2.3.2 实验培养条件.- 9 -2.3.3 9 号小球藻细胞生长标准曲线的测定.- 10 -2.3.4 9 号小球藻生物量的测定.- 10 -2.3.5 9 号小球藻藻细胞的收集.- 10 -目录IV2.3.6 9 号小球藻总脂含量的测定.- 10 -第三章 结果与讨论.- 12 -3.1 9 号小球藻的生长曲线.- 12 -3.1.1 9 号小球藻细胞形态的观察.- 12 -3.1.2 9 号小球藻藻细胞生长的测定.- 12 -3.2 9 号小球藻的总脂含量.- 17 -3.2.1 9 号小球藻总脂含量的测定.- 17 -3.2.2 对利用 9 号小球藻进行工业化生产分析结果。.- 17 -第四章 结论.- 18 -参考文献.- 19 -致 谢.
