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1、Protein purification and preparation. High purity and recovery for better discovery. IntroductionToday, researchers are challenged to create high quality protein samples for successful proteome analysis, often using cumbersome traditional sample preparation methods. With over 50 years of experience
2、in developing protein sample preparation technologies, Merck Millipore is constantly innovating new tools to offer you rapid and efficient solutions that can be smoothly integrated into your workflow. Why spend your time on arduous sample preparation protocols when you can focus your efforts on exci
3、ting experiments? With the right pure protein, in the buffer you need, at the concentration you want, your next discovery is only a step away. From protein isolation to purification, you can count on Merck Millipore to support your research.Unmatched FlexibilityIsolate proteins from a diverse range
4、of sample types with our flexible, broad range of kits. Multiple downstream applicationsOur kits enable you to produce samples that can be used directly in applications such as activity assays, protein microarrays, SDS-PAGE, immunoblotting, ELISA, two-dimensional gel electrophoresis (2DGE), mass spe
5、ctrometry (MS; including MS/MS, LC-MS, MALDI-MS, SELDI-MS, and ESI-MS), and others. Scale-up compatibilityIts easy to scale up to high-throughput recombinant protein purification and solubility screening using our sample preparation reagents.Key FeaturesCell Lysis Kits and Reagents (“Busters”) .6 Fa
6、st, simple, gentle protein extraction from E. coli, yeast, insect and mammalian cellsProteoExtract Kits .8 Extract proteins from different fractions of the cell, including membrane, nucleus, cytosol and cytoskeletonCell Lysis no centrifugation required.YEASTYeastBuster Protein Extraction ReagentEffi
7、cient protein extraction from yeast under non-denaturing conditions from any volume of culture. Add 0.5 M THP Solution (included) and Benzonase Nuclease for enhanced efficiency.INSECTCytoBuster Protein Extraction Reagent*Gentle lysis of insect cells with retention of protein activity for assays and
8、purification. Can use with monolayers or pellets derived from suspension cultures.Insect PopCulture ReagentLysis of insect cells directly in serum-free medium. Ideal for expression screening of many small samples.MAMMALIANCytoBuster Protein Extraction Reagent*Gentle lysis of mammalian cells with ret
9、ention of protein activity for assays and purification. Can use with monolayers or pellets derived from suspension cultures.ProteoExtract Kits*Extract protein fractions from different parts of the cell. A range of kits offering maximum flexibility.LYSIS AND EXTRACTION ENHANCEMENTGram-negative bacter
10、ia (E. coli)rLysozyme SolutionCleaves bond in peptidoglycan layer of E. coli cell wall. Lysonase Bioprocessing ReagentConvenient mixture of rLysozyme and Benzonase Nuclease minimizes pipetting steps.Gram-positive bacteriaChicken Egg White Lysozyme SolutionCleaves bond in peptidoglycan layer of bacte
11、rial cell wall.All cellsBenzonase NucleaseDegrades all types of nucleic acids for more efficient protein extraction, faster chromatography, and reduced interference in assays.1D PAGE One-dimensional Polyacrylamide Gel Electrophoresis 2D PAGE Two-dimensional Polyacrylamide Gel Electrophoresis IEF Iso
12、electric Focusing * Salt must be removed before IEF600.0050.0100.0150.0200.0250.0300.0350.0400.045Sonication Another BugBuster ReagentGST Activity (A340/min)Protein Extraction with Cell Lysis Reagents (“Busters”)Featured ProductsBugBuster Protein Extraction Kits and Reagents Simple extraction of sol
13、uble protein from E. coli without sonicationGently disrupt the cell wall of E. coli and liberate soluble proteins with BugBuster Kits and Reagents. BugBuster reagent provides a simple, rapid, low-cost alternative to mechanical methods such as French press or sonication for releasing expressed target
14、 proteins in preparation for purification or other applications. The proprietary formulation uses a detergent mix to perforate cell walls without denaturing soluble protein. Simply harvest cells by centrifugation and suspend in BugBuster reagent. Following a brief incubation, remove insoluble cell d
15、ebris by centrifugation. The clarified extract is ready to be purified.Lysis MethodHigher protein yield (as measured by activity of recombinant glutathione S-transferase (GST) using BugBuster reagent compared to other E. coli lysis methods. 50 mL samples of an induced 500 mL culture of BL21(DE3) containing pET-41a(+) encoding GST were harvested by centrifugation and resuspended in 2 mL 1X phosphate-buffered saline (PBS), another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s tota
