《thermalcyclerprojectplan》由会员分享，可在线阅读，更多相关《thermalcyclerprojectplan（25页珍藏版）》请在金锄头文库上搜索。
1、1JGI THERMAL CYCLER PROJECTDesignWorks team members:Ken Chow Lisa Gullo2TABLE OF CONTENTSPROJECT SUMMARY KEY ISSUES.4THERMAL CYCLER PROJECT PLAN.5FIRST MEETING SUMMARY 4/30/01.6KEY POINTS.6BRAINSTORMING SESSION RESULTS .6CONCEPT SUMMARY FOR CONCEPT 1: STRAIN GAGE SYSTEM.6 DESCRIPTION OF CONCEPT.6CON
2、CEPT SUMMARY FOR CONCEPT 2: PRESSURE SENSITIVE FILM SYSTEM.7 DESCRIPTION OF CONCEPT.7RESOURCES FOR PRESSURE SENSITIVE FILM.8CONCEPT SUMMARY FOR CONCEPT 3: TEKSCAN FORCE backing paper not removed 22 384-73GreinerNormalNominal + 1/2 turn23 384-73GreinerNormalNominal + 1 turnBacking paper not removed;
3、Smudging may be result of platen sliding 24 384-74GreinerNormalNominal + 1 turn18TEST SAMPLES SAMPLES 1-419SAMPLES 5-820SAMPLES 9-1221SAMPLES 13-1522SAMPLES 17-2023SAMPLES 21-2424INVENTORY DesignWorks has the following remaining tools in inventory:ItemQuantity Thermal cycler1 Thermal cycler operatio
4、ns manual1 Thermal cycler service manual; Chapter 11 Microplate1 Paintbrush1 Pressurex Micro carbon paper1 sheet Fuji Prescale film: Ultra low: 28-85 psi4 sheets 1 foot long Fuji Prescale film: Super-low: 70-350 psi4 sheets 1 foot long Tekscan pressure sensors 4 Female adaptors4WHATS NEXT? 1)Improve
5、 procedure by increasing the size of the sample Pressurex paper so that there is a longer pealing edge to minimize sample smearing. 2)Determine how many types of Microplate batches are used. Is there variation amongst each of those batches of microplates? 3)Determine how the microplates are used. Ar
6、e the microplates discarded or reused? 4)Provide recommendation to engineers and technicians to set the thumbscrew pressure as high as possible.5)Determine the effect of the sealing film. What kind of material is it made out of? How is it applied and removed?6)Further tests with the possible followi
7、ng modifications: a.peal off rubbery silicone backing b. determine the grade of silicone that we are currently using, replace silicone bonnet lid with different grades of silicone c.add additional layer of silicone to the platen d. test all of the representative batches of microplates e.test individ
8、ual microplates within each batch to see if there is variation7)Identify goals: a.Do we want to calibrate microplates or thermal cyclers or both? b. Calibrating the microplates will not be effective if they are discarded and/or changed frequently25c.Calibrating individual thermal cyclers may not be
9、effective if the problem is with the microplates d. Do we want to retrofit the bonnet of the thermal cycler to improve pressure?FOLLOW UP LETTERJune 15, 2001Marty,Enclosed is the Pressurex Micro carbon paper along with some of the Microfilm paper. Ive also included the manufacturer directions and a
10、paintbrush to use for application. There is some remaining higher rated microfilm paper. If youd like us to ship you the remainder, we will do so. Here is the contact information for the vendor:Sensor Products, Inc. 188 Route 10, Dept 307 East Hanover, NJ 07936-2108Phone: (973) 884-1755 Fax: (973) 884-1699This order was placed under: Procard P.O. # 99477 Web Job Order # UC0645Please let me know if you have any questions.Regards,Lisa Gullo (510) 486-4006
