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1、LED光源在园艺与药用植物和 人工虫草室内培养中的应用华南师范大学生命科学学学院 李海航,博士、教授、博导 2014年6月21日，广州广东省植物生长调节剂协会与广州市果树科学研究所2014年植物生长调节剂与现代农业研讨会植物的种子萌发、幼苗生长、形态建成、开花结果等过程都需要光不同的生物及不同生长发育期需要不同的光质与光强降温光在植物与食用菌等的室内培养中广泛应用光照研究内容非洲菊组织培养 不定芽诱导、芽增殖和生根铁皮石斛和金钗石斛组培繁殖 原球茎增殖、生长、分化虫草子实体形成,生长,功能成分 子实体的诱导形成 生长：高、鲜重、干重 生物活性物质: 虫草素,多糖,类胡萝卜素Explant:De
2、differentiationcallusRedifferentiationSingle shootCluster shootsRootingPlantletsMultiplicationDivisionAdventitious budsLED treatmentShoot multiplication procedureCompared to WFL, no significant differences were observed in the treatments of V1,V2, V5 and V6. V3 and V4 decreased the FW of the shoots.
3、 V6 increased dry weight significantly.Fresh weight and dry weightHeight and number of shootsAll LEDs could increase the height of shoots except V5. But No.of shoots/explant had no significant different.Chlorophyll contentAll LEDs induced chlorophyll biosynthesis significantly, compared with WFLWV1V
4、2V3V4V5V6WFL35 days cultured in vitro under LEDV1V4V3V6V2V5WFL35 days cultured in vitro under LEDExplant:DedifferentiationcallusRedifferentiationRootingPlantletsResults Root inductionReceptacleMultiplicationDivisionAdventitious budsCluster shootsRoot induction under LEDSingle shootV3 treatment decre
5、ased FW and DW.V2 treatment increased FW and DW significantly.Fresh weight and Dry weightHeights of shoots under V1, V2, V3 and V4 were significantly higher than control.V2 had the most number of roots compared with others.Shoot height and root numberV3, V4 and V5 promoted the root activity signific
6、antly.V2 increased the maximum root length.Root activity and maximum root lengthV1V2V3V4V5V6WFLSeedlings 35 d after rooting inductionSurvival rateHeight (cm)No. of leavesV1V2V3V4V5V6WFL100%100%100%100%100%100%100%9.460.22 a 9.770.22 a8.190.34 b9.540.30 a8.020.27 b8.250.48 b7.090.23 c5.190.23 b 4.940
7、.11 b6.060.19 a6.000.35 a5.310.24 b6.190.20 a4.600.27 bResults Transplantation15 days after transplantation, no difference on seedlings survival rates.All LED treated seedlings were significant higher and number of leaves increased.V4V1V5V2V6V3WFLTransplanted seedlings grown for 15 days Among 6 LEDs
8、, V1,V2 and V6 were suitable for shoot multiplication and V2 was good for root induction. Survival rates of seedlings from all LED treatments had no difference after seedling transplantation. But the seedlings grown under LEDs were higher with more leaves than WFL.ConclusionsJie Ye 叶婕Hongmei Bao 包红梅
9、QingshengYe 叶庆生II - Dendrobium officinale组培繁殖Tissue culture processshootsPlantlets(1-2cm)seedling (7-8cm)germination1 monthgrowth and differentiation2 monthseedsgrowth1 monthprotocormsshoot elongation3 monthPlantlets27LED treatmentFW of plantlet(g)bc abc c cAfter 90 days growth, FW of plantlet under
10、 V6 was the highest, but there were no significant differences compared to control.30 days60 days90 days0.10.00.20.3V1 V3V2 V4 V6V5 FLad daabaabababaaba c cbcabFW of plantletsV6DW of plantlet(g)DW of plantlets under V6 was significantly higher than others after 90 days treatment.30 days60 days90 day
11、s0.000.010.020.03ab ababbaaaa a aaV1V2 V3V4 V6V5 FLaba cabcaba a abDW of plantletscV6Height of plantlet(cm)There were some different in plantlet height after 30 and 60 days irradiation under LED, the different disappeared after 90 days.30days60days90days0.50.01.52.0V1V2 V4 V6V3 V5 FLaaaabab aac bca
12、aaa abcbc abcbcHeight of plantletsa90天后差异不显著Stem diameter (cm)bcbc cdSimilar results were observed in stem diameter30days60days90days0.00.20.3V2 V4 V6V1 V3 V5 FLd cd aababbdab aa aa abab abababStem diameter V6TilleringCompared with other treatments, number of tillering under V6 was significantly hig
13、her than FL.60days90days012a aV1V2 V3V4 V6V5 FLa aaabbcabc bcdcaTillering分蘖V6V6Roots of plantletAfter 60 days cultivation, there were more roots under V6.60days90days204V1 V3V2 V4 V5V6 FLa aaaacdaab bdabcaRoots number per plantletsaGrowth of plants after 30days under LED.Growth of plants after 60 da
14、yscultivation under LED.Growth of plants after 90 dayscultivation under LED. Among 6 LED, V6 were suitable for plantlet growth, it promoted the DW, diameter, tillering and rooting in Dendrobium officinale.ConclusionsIII - Cordyceps militarisZhiliYi 易智莉Haihang Li 李海航本实验室培养 的蛹虫草选出对蛹虫草的生长、产品质量最优的光质， 为L
15、ED光源在蛹虫草栽培中的运用提供理论和应用基础。不同的光照时间蛹虫草色素的变化初步探究虫草色素的光诱导形成过程与机理。重 复 3 次原基诱导形成材料与方法材料与方法重 复 3 次子实体生长实验材料与方法甲醇浸提光照组组合光质质红红/蓝蓝/远红远红1远红远红 光/2红红光/3红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 4244红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 3255红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 3346红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 1457红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 1638蓝蓝光/9白光红红蓝蓝远红远红 =
16、63110荧荧光灯（对对照）红红蓝蓝远红远红 = 460LED光照处理结果与讨论R FrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/31. 黑暗、红红光、远红远红 光下不能形成 子实实体原基2. 蓝蓝光和含蓝蓝光的LED组组合都能 诱导诱导 原基形成。表明蓝蓝光在子实实 体原基诱导诱导 形成中起决定性作用3. LED白光（R/B=2/1）和 LED处处 理（R/B=2/1）对对原基的诱导诱导 形 成较较好1.光质对子实体原基形成的影响R FrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/3 处处理鲜鲜重最高 其他LED处处理组组与荧荧光对对照组组 无显显著差异 远红远红 光下鲜鲜重最低结果与讨论2.光质对子实体生长（鲜重）的影响R FrBWFLR/B/Fr=4/2
