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1、基金项目:国家自然科学基金青年基金项目(No. 30500678) ;霍英东基金会高等院校青年教师基金(No. 02462052FN360009) ;复旦大学上海医学院青年骨干科研启动基金项目(No. 07J03)Correspondence: Yan2qing WANG, PhD , Associate Professor ; Tel : 021254237496 ; E2mail : wangyanqing shmu. edu. cnOriginal Experimental Research 实验论著 电针联合西乐葆缓解大鼠胫骨癌痛毛应启梁1,任典寰1,米文丽1,刘琼1,王彦青1 , 2
2、 , 31.复旦大学上海医学院中西医结合系,上海2000322.复旦大学脑科学研究院针刺原理研究所,上海2000323.复旦大学医学神经生物学国家重点实验室,上海200032目的:建立大鼠胫骨癌痛模型,观察电针和西乐葆对大鼠胫骨癌痛的缓解作用。 方法:采用经皮穿刺技术将大鼠乳腺癌细胞Walker 256接种至雌性Wistar大鼠胫骨骨髓腔内,建立大鼠胫 骨癌痛模型。大鼠造模后,采用电针和西乐葆灌胃治疗,观察其对大鼠骨肿瘤引起的机械性痛觉超敏的影 响。 结果:模型组大鼠后肢逐渐产生机械性痛觉超敏,接种后第16天胫骨近心端可见显著的肿瘤生长。单用电针或西乐葆5 mg/ (kgd)治疗大鼠,与模型组
3、相比其机械性疼痛阈值差异无统计学意义;治疗后第22天和 第26天,西乐葆10 mg/ (kgd)组大鼠后肢机械性疼痛阈值较溶剂对照组提高(P 0. 05)。见图2。图2 胫骨癌痛模型大鼠后肢机械性痛超敏的变化情况Figure 2 Changes of mechanical allodynia of the ipsilateralhind paw in rats with tibial cancer pain3 3P 0. 05)。见图3。图3 电针对胫骨癌痛大鼠机械性痛觉超敏的影响Figure 3 Effects of EA on mechanical allodynia of theipsi
4、lateral hind paw in rats with tibial cancer pain2. 4 不同剂量西乐葆对大鼠胫骨癌痛时机械性痛 觉超敏的影响 治疗后第22和26天,西乐葆10 mg/ (kgd)组大鼠后肢机械性疼痛阈值高于MC对照组(P 0. 05)。见图4。图4 不同剂量西乐葆对胫骨癌痛大鼠机械性痛觉超敏的影响Figure 4 Effects of different doses of Celebrex onmechanical allodynia of the ipsilateralhind paw in rats with tibial cancer pain3P 0.
5、 05)。 在治 疗后第6、18和23天,电 针 合 用 西 乐 葆5 mg/ (kgd)组大鼠机械性疼痛阈值高于模型组 大鼠,差异有统计学意义(P 0. 05) ,提示 多次电针未能有效缓解胫骨癌痛大鼠机械性痛觉超 敏。这一结果提示电针在癌痛模型上的镇痛作用具有一定的局限性。实践证实,针药结合优势互补,是提高镇痛效应 的重要途径4。因此本研究采用电针和本来没有显 著镇痛作用的西乐葆5 mg/ (kgd)合用观察对大 鼠胫骨癌痛的镇痛作用。研究表明,单用电针或单 用西乐葆5 mg/ (kgd)对大鼠胫骨癌痛时的机械 性痛阈与模型组比较,差异无统计学意义(P0. 05) ,而两者合用治疗一段时间
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