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1、 维生素维生素 C C 论文：维生素论文：维生素 C C 纳米脂质体的制备及其性质的研纳米脂质体的制备及其性质的研究究【中文摘要】维生素 C 是一种水溶性维生素,具有美白祛斑、延缓衰老的美容功效,但是容易氧化变质,且不易被皮肤吸收。脂质体作为一种新兴药物载体,对包封的药物具有保护作用,从而提高其作用时间。本课题以水溶性物质维生素 C 为模型材料,采用动态高压微射流(DHPM)作为核心技术,结合传统脂质体制备方法,通过工艺优化,制备了维生素 C 脂质体和维生素 C 纳米脂质体,并研究了这两种脂质体的物理化学性质(粒径、包封率、外观形貌、稳定性、缓释性)和生物利用率(抗氧化能力和皮肤渗透性)。本课
2、题建立了测定维生素C 脂质体包封率的分析方法：采用透析法分离游离维生素 C、甲醇破乳、紫外分光光度法测定包封率。制备了维生素 C 脂质体和维生素C 纳米脂质体,考察了工艺配方因素的影响。结果表明,维生素 C 脂质体的最优制备条件为：制备温度为 65,维生素 C 浓度为 2 mg/ml,维生素 C 和总脂材质量比为 1：25,卵磷脂和胆固醇质量比为 5：1,表面活性剂和总脂材质量比为 2：10,卵磷脂和无水乙醇质量体积比为2.5：100。制备维生素 C 纳米脂质体的最优条件为：制备温度 60,维生素 C 浓度 5 mg/ml,维生素 C 和总脂材质量比为 1：10,卵磷脂和胆固醇质量比为 4：1
3、,表面活性剂和总脂材质量比为 4：10,卵磷脂：无水乙醇=10：100,处理压力为 140 MPa,处理 2 次。对维生素 C 脂质体和维生素 C 纳米脂质体的理化性质、缓释性和稳定性进行评价,结果表明：维生素 C 纳米脂质体较好,包封率为(47.166.28)%,平均粒度为(73.94.4)nm,微观形貌呈实体球状,贮藏稳定性、体内稳定性良好,缓释作用明显。对比研究了维生素 C 脂质体和维生素 C 纳米脂质体的抗氧化功能和皮肤穿透能力,结果表明,两种脂质体的维生素 C 抗氧化功能和游离维生素 C 基本一致；皮肤渗透能力的排列顺序为：维生素 C 纳米脂质体维生素 C 脂质体游离维生素 C。【英
4、文摘要】Vitamin C is water soluble,it has the effect of skin lightening and anti-aging,but it is easy to oxidative deterioration, and not easily penetrate through the skin. As a new drug carrier,liposomes has the effect of protecting the core material encapsulated and improving the bioavailability of dr
5、ugs.As the core material, Vitamin C was prepared to Vitamin C liposomes and Vitam in C nanoliposomes using dynamic high-pressure microfluidization (DHPM) as a core technology which combined with the traditional method of liposomal preparation. In addition, both the physical and chemical properties o
6、f liposomes (mean particle size, encapsulation efficiency, morphology, stability, time-controlled releasing,) and bioavailability (antioxidant capacity and skin permeability.) were characterized.The analysis method of determinating entrapment efficiency of vitamin C liposomes was established:separat
7、ed free vitamin C by dialysis, breaken the liposomes by methanol emulsion, determinated Vitamin C content by UV spectrophotometry.The traditional liposomal preparation methods and DHPM combined with the traditional method of liposomal preparation were developed to prepare vitamin C liposomes and vit
8、amin C nanoliposomes, respectively. Each factor was analyzed and the orthogonal test was taken to optimize the formula.The optimized formula of vitamin C liposomes as follows:the preparation temperature was 65,the concentration of vitamin C was 2 mg/ml, the mass ratio of vitamin C and lipids was 1:2
9、5, the mass ratio of phospholipid and cholesterol was 5:1, the mass ratio of Tween-80 and lipids was 2:10,the mass volume ratio of phospholipid and ethanol was 2.5:100. The optimized formula of vitamin C nanoliposomes as follows:the preparation temperature was 60,the concentration of vitamin C was 5
10、 mg/ml, the mass ratio of vitamin C and lipids was 1:10, the mass ratio of phospholipid and cholesterol was 4:1, the mass ratio of Tween-80 and lipids was 4:10,the mass volume ratio of phospholipid and ethanol was 10:100,the treatment pressure and times were 120 MPa and 4.The physicochemical propert
11、ies of both vitamin C liposomes and vitamin C nanoliposomes were evaluated.Vitamin C nanoliposomes had advantages over vitamin C liposomes with the encapsulation efficiency(47.166.28%),the mean particle size(73.94.4 nm),showed a solid spherical morphology,good storage and vivo stability,significantl
12、y slow release.Compared the antioxidant capacity and the skin penetration of vitamin C liposomes and vitamin C nanoliposomes, results showed that, compared to free vitamin C,the antioxidant capacity of two types of liposomes was not affected; he order of skin penetration was:Vitamin C nanoliposomes
13、Vitamin C liposomes Free Vitamin C.【关键词】维生素 C 动态高压微射流 纳米脂质体 稳定性 皮肤渗透【英文关键词】Vtamin C DHPM Nanoliposomes Stability Skin penetration【目录】维生素 C 纳米脂质体的制备及其性质的研究 摘要 3-4 ABSTRACT 4-5 第一章 前言 9-28 1.1 脂质体研究现状 9-23 1.1.1 脂质体简介 9-11 1.1.2 脂质体分类 11-12 1.1.3 脂质体的特点 12-13 1.1.4 脂质体的制备方法 13-16 1.1.5 纳米脂质体的概述 16 1.1
14、.6 脂质体理化性质的评价 16-17 1.1.7 脂质体的应用概况 17-23 1.2 维生素 C 简介 23-24 1.2.1维生素 C 的结构及性质 23 1.2.2 维生素 C 的美容功效 23-24 1.3 动态高压微射流技术简介 24-25 1.3.1 动态高压微射流技术概述 24 1.3.2 动态高压微射流技术国内外研究现状 24-25 1.4 选题意义 25-26 1.5 研究内容及创新点 26-28 第二章 维生素 C 含量及脂质体包封率测定方法的建立 28-35 2.1 主要试剂及仪器 28-29 2.2 实验方法 29-31 2.2.1 破乳剂的确定 29 2.2.2 维
15、生素 C 的测定 29-30 2.2.3 分离方法的筛选 30-31 2.2.4 维生素 C 脂质体包封率的测定 31 2.3 结果与讨论 31-34 2.3.1 破乳剂的确定 31-32 2.3.2 维生素 C 的测定 32 2.3.3 透析法测定维生素 C 脂质体的包封率 32-34 2.4 本章小结 34-35 第三章 传统方法制备维生素 C 脂质体的研究 35-47 3.1主要试剂及仪器 35-36 3.2 实验方法 36-38 3.2.1 制备方法的筛选 36-37 3.2.2 实验设计 37-38 3.3 结果与讨论 38-46 3.3.1 制备方法的筛选 38-39 3.3.2 单因素实验结果 39-43 3.3.3 正交实验结果 43-45 3.3.4 验证实验结果 45-46 3.4 本章小结 46-47 第四章 动态高压微射流技术制备维生素 C 纳米脂质体的研究 47-61 4.1 主要试剂及仪器 47-48 4.2 实验方法 48-51 4.2.1 制备方法的筛选 48-50 4.2.2 实验设计 50-
