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1、 对改良小麦品质的正面影响探究小麦贮藏蛋白决定小麦的品质特性，决定面团的弹性和延伸性。高分子量麦谷蛋白亚基（HMW-GS）仅占小麦贮藏蛋白的 10%，但对加工品质起着决定性作用。HMW-GS由染色体 1A、1B、1D 长臂上的位点控制，这些位点总称为 GLu-1 位点。不同 HMW-GS 亚基对面团特性和烘烤品质有着不同的影响。Glu-A1 编码的 1 和 2*亚基，Glu-B1编码的 7+8、17+18、13+16 和 14+15 亚基以及 Glu-D1 编码的 5+10 亚基均对面包加工品质有正向作用1-2。近年研究发现，1Bx 基因的复制导致 7 亚基的超量表达，这可以显着提高面团强度
2、6-7。国内对 7OE 亚基也进行了初步研究。张平平等选用 13 份含 7OE 亚基姊妹系材料，利用反相高效液相色谱分析法（RP-HPLC）和凝胶色谱（SE-HPLC）方法研究了含 Glu-B1al（7OE8）材料的 HMW-GS 总量和面团强度。任妍等利用两对 STS 引物验证了检测 7OE 引物的特异性，为其快速检测提供了方法。 Wheat storage protein determines the quality characteristics of wheat dough, decided the flexibility and extensibility. High molecul
3、ar weight glutenin subunits ( HMW-GS ) accounted for only 10% of wheat storage proteins, but the processing quality plays a decisive role in. The HMW-GS is controlled by chromosome 1A, 1B, 1D on the long arm of http:/ http:/ http:/ http:/ the sites, these sites are collectively referred to as the GL
4、u-1 site. There are different effects of different subunits of HMW-GS properties of dough and baking quality. The Glu-A1 code of 1 and 2* subunits, Glu-B1 encoding 7+8, 17+18, 13+16 and 14+15 subunits and Glu-D1 encoding the 5+10 subunit have the positive effect of 1-2 on the processing quality of b
5、read. Recent studies have found, the overexpression of 1Bx gene copy in 7 subunit, which can significantly improve the dough strength 6-7. China has conducted a preliminary study of 7OE subunit. Zhang Ping equal selection of 13 with 7OE subunit Sister Lines materials, analysis by reversed-phase high
6、 performance liquid chromatography ( RP-HPLC ) and gel permeation chromatography ( SE-HPLC ) method with Glu-B1al ( 7OE + 8 ) materials HMW-GS volume and dough strength. Ren Yan, using two pairs of STS primers to verify the specificity for detection of 7OE primer, for its rapid detection provides a
7、method.矮败小麦是用“矮变 1 号”给太谷核不育小麦授粉，F1不育株再用高秆品种测交所得，中国自主创新的重大科技成果。矮败小麦的后代群体中一半是异交结实的矮秆雄性不育株和一半自交结实的非矮秆可育株，是理想的http:/ http:/ http:/ http:/ 轮回选择工具。津强 5 号品质达国家一级强筋小麦标准，且各项品质指标与加麦和硬红春相仿，经张平平等检测含 7OE 亚基。 Dwarf Male-sterile Wheat with “ aibian No. 1 “ for pollination of Taigu genic male sterile wheat, F1 male
8、-sterile plants and tall cultivars measured income, major scientific and technological achievements of independent innovation of china. Dwarf male sterile wheat progenies in the half is outcrossing dwarfing male sterile lines and half the self-fruitful non dwarf fertile plants, is ideal for recurren
9、t selection tool. Jinqiang 5 quality reached the national standard of strong gluten wheat, and every quality index and game-design and hard red spring is similar, the Zhang Ping equal detection with 7OE subunit.Ragupathy 等根据 LTR 逆转座子边界与重复片段的结合区域设计了两个 STS 标记并检测了 400 多份小麦品种，经任妍研究能有效鉴定 7OE 基因，这为快速准确检测
10、7OE 基因在本群体中的分布提供了可能。本研究对195 份矮败小麦与津强 5 号杂交得到的高代品系 7OE 亚基进行分子检测，塑料土工格栅明确此位点等位基因的分布规律，并检测这批材料的揉混特性，为我国小麦育种提供有用的材料以及分子标记辅助选择方法。 http:/ http:/ http:/ http:/ Ragupathy according to the LTR retrotransposon boundary and repetitive fragment binding region designed two STS markers and 400 wheat varieties tes
11、ted, the Ren Yan research can effectively identify the gene 7OE, which provides possibility for the distribution of rapid and accurate detection of 7OE gene in the population of. The study of 195 Dwarf Male-sterile Wheat and Jinqiang 5 were high generation strain 7OE subunit molecular detection, dis
12、tribution of the alleles, and detect the mixing characteristics of the materials, provide materials and molecular marker assisted selection method useful for wheat breeding in china.1 材料和方法 Materials and methods 11.1 供试材料 1.1 materials195 份以津强 5 号作为轮回亲本与矮败小麦回交 2 次的高世代品系，2009 年种植于天津市武清区周庄村，每区 2 行，行长
13、2 m，行距 30 cm，5 cm 点播。对其中 9 份农艺性状优良梨苗的非 7OE 亚基和 6 份含 7OE 亚基材料于 2010 年进行进一步扩繁，分析其揉混特性。 195 copies in Tianjin strong 5 as recurrent parent and Dwarf Male-sterile Wheat backcross high generation line 2 times, http:/ http:/ http:/ http:/ 2009 plantings in Tianjin Wuqing District Zhouzhuang village, each
14、district 2, m, 2, row spacing of 30 cm, 5 cm. The 9 excellent agronomic characters of non 7OE subunits and 6 7OE subunit containing materials for further expansion in 2010, analyzes its mixing characteristics.1.2 基因组提取 1.2 genomic DNA extraction采用 SDS 法提取小麦基因组 DNA。每份材料分别提取二粒种子的 DNA，利用紫外分光光度计检测 DNA 浓
15、度，终浓度调整至 20 ngL-1。 Extraction of wheat genomic DNA by SDS method. Each material were extracted from two seed DNA, using ultraviolet spectrophotometer to detect the DNA concentration, the final concentration was adjusted to 20 ng L-1.1.3 STS 标记检测 1.3 STS markers利用 Ragupathy 等开发的显性 STS 标记检测 Bx7OE基因。引物由天津润泰科技发展有限公司合成。 The detection of Bx7OE dominant STS marker gene Ragupathy development. Primers by Tianjin rint Technology Development Co Ltd synthesis.http:/ http:/ http:/ http:/ 标记（重复片段与逆转座子左边结合引物）：Ta
