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1、西安源森生物科技有限公司 400-088-6586西安源森生物科技有限公司 微信公众平台 yuensen西安源森生物科技有限公司苦杏仁甙检测方法西安源森生物科技有限公司苦杏仁甙检测方法1. Instrument 设备设备 a HPLC-9a (Shimadzu) b Sartorius electronic balance (Germany) 电子称c. KQ-model 100, Washing device of ultrasonic wave 100-KQ 超声波洗涤设备2. Condition 条件条件Chromatographic Column 色谱柱 ROM-150 ODS-c18
2、(4.6mm150mm, 5)Mobile Phase: Acetonitrile:Water:Acetic acid =2080:0.1 流动相:乙腈:水:醋酸=2080:0.1(V/V/V) Flow Rate: 1.0mL/min 流速Wave Length: 220nm 波长Column: room temperature 测柱: 室温3.Standard solution 标准溶液标准溶液 Transfer about 8 mg amygdalin standard(Sigma company U.S.A.), accurately weighed, to a 25mL volume
3、tric flask, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water. Then add water to scale mark, it is obtained.精确称量 8mg(美国 sigma 公司)标准苦杏仁甙,放入 25ml 的量杯, 加适量水溶解,用超声波震动直至全溶。 然后将水加至标度处,获得标准溶液。 4.Testing solution 测试溶液测试溶液Transfer 8mg amygdalin pow
4、der, accurately weighed, to a 25ml volumetric flask, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water, Then add water to scale mark, shake evenly, it is obtained.精确称量 8mg 苦杏仁甙粉末,放入 25ml 的量杯中,加入适当水溶解,用超声波摇动直至溶解。然后加入水至标度处,均匀摇晃,获得测试溶液。 5. Pr
5、ocedure 过程过程 Separately inject equal volumes (about 20 uL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks. A1 W2X%=- - 100%A2 W1In which is A1 the assay sample , A2 is the standard peak area, W2 is the concentration of standard, W1 is the concentration of assay sample.将等量的(大约 20 UL)标准溶液和测试溶液(大约 20 UL)分别注入色谱仪,记录两个色相图谱 测量两个主峰的反应变化。A1 W2X%=- - 100%西安源森生物科技有限公司 400-088-6586西安源森生物科技有限公司 微信公众平台 yuensenA2 W1上式中,A1 指测试样的峰面积,A2 是标准溶液的峰面积, W2 指标准溶液的浓度,W1 指测试样的浓度。