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1、Medical Bacteriology Junqi Zhang(张俊琪张俊琪), Ph.D.Junqi Zhang(张俊琪张俊琪), Ph.D.E- mail: Tel: 13918199186 Department of Medical Microbiology, Key laboratory of Medical Molecular Virology, Ministry of Education and Ministry of Health School of Basic Medical Sciences, Fudan University2015/5/7Staphylococcus-
2、 Junqis Lecture for Medical Students2化脓性球菌化脓性球菌 (Pyogenic cocci)StaphylococciStreptococciNeisseria32015/5/7Staphylococcus- Junqis Lecture for Medical StudentsSmear v为非溶血性毒素; v只杀伤白细胞; v使细胞膜上三磷酸肌醇变构，影响细胞膜 电位的稳定性。222015/5/7Staphylococcus- Junqis Lecture for Medical Students葡萄球菌溶素（葡萄球菌溶素（staphlolysin, c
3、ytolytic toxin） v 分为,其中 对人致病主要为主要为；v 为蛋白质,不耐热, 可被甲醛脱毒为类 毒素；毒素；v 产此毒素的葡萄 球菌在血平板上菌 落周围出现溶血 环。v 破坏细胞膜,损伤 各种血细胞和组织 细胞。232015/5/7Staphylococcus- Junqis Lecture for Medical StudentsMicrobiol Rev 1991;55:733- 735242015/5/7Staphylococcus- Junqis Lecture for Medical Students引起烫伤样皮肤综合征引起烫伤样皮肤综合征 （Staphylococc
4、al Scalded Skin Syndrome, SSSS）表表皮剥脱毒皮剥脱毒素（素（exfoliative toxin）A型由噬型由噬菌菌体编码体编码， B型由型由质质粒编码粒编码252015/5/7Staphylococcus- Junqis Lecture for Medical StudentsInfect. Immun. vol. 68 no. 9 5044- 5049 2015/5/7Staphylococcus- Junqis Lecture for Medical Students26Toxins (Basel) (2010)毒毒性性休克综合征毒休克综合征毒素素- 1（ t
5、oxic shock syndrome toxin- 1, TSST- 1）引起机体发热引起机体发热，增加增加对内对内毒毒素的敏感性素的敏感性； 超超抗抗原原，引起多器官系统功能紊乱或毒引起多器官系统功能紊乱或毒性性休休 克综合征克综合征 （TSS）The gene encoding toxic shock syndrome toxin is carried by a mobile genetic element of S. aureus in the SaPI family of pathogen city islands. 272015/5/7Staphylococcus- Junqis
6、Lecture for Medical Students282015/5/7Staphylococcus- Junqis Lecture for Medical Students毒毒性性休克综合征休克综合征（ toxic shock syndrome, TSS）Following controversial test marketing in Rochester, New York and Fort Wayne, Indiana, in August 1978, Procter and Gamble introduced superabsorbent Rely tampons to the U
7、nited States market in response to 2015/5/7Staphylococcus- Junqis Lecture for Medical Students291.Rely used carboxymethylcellulose (CMC) and compressed beads of polyester for absorption. 2.This tampon design could absorb nearly 20 times its own weight in fluid.3.Further, the tampon would “blossom“ i
8、nto a cup shape in the vagina to hold menstrual fluids without leakage. United States market in response to womens demands for tampons that could contain an entire menstrual flow without leaking or replacement. 白带分泌物革兰氏染色：白带分泌物革兰氏染色： 乳酸杆菌乳酸杆菌，G+302015/5/7Staphylococcus- Junqis Lecture for Medical St
9、udents乳酸杆菌乳酸杆菌，肠毒肠毒素素 （enterotoxin）约约1/3临床分临床分离离金黄色葡萄球菌金黄色葡萄球菌可可产生产生引起急引起急性性胃肠炎胃肠炎（即食即食物物中毒中毒）；分A, B, C1, C2, C3,D,E, G, 和 H 9个血清型。MW 26- 30 kDa。耐耐热热（100，30min，抗，抗胃肠液中胃肠液中蛋蛋白酶白酶）作用机制作用机制：刺激呕吐刺激呕吐中中枢导枢导致致以呕吐以呕吐为主要为主要症状症状的的食食物物中毒中毒还具有还具有超超抗抗原原（superantigen）作用作用312015/5/7Staphylococcus- Junqis Lecture
10、for Medical Students 所致疾病所致疾病2015/5/7Staphylococcus- Junqis Lecture for Medical Students32侵袭侵袭性性局部局部感染感染全全身身感染感染 所致疾病所致疾病脓脓汁黏稠汁黏稠，病，病灶局限灶局限毒毒素性素性食食物物中毒中毒TSSSSSS332015/5/7Staphylococcus- Junqis Lecture for Medical Students化脓性感染特化脓性感染特点点：脓脓汁粘稠汁粘稠，病，病灶局限灶局限2015/5/7Staphylococcus- Junqis Lecture for Med
11、ical Students34特特点点：脓脓汁粘稠汁粘稠病病灶局限灶局限2015/5/7Staphylococcus- Junqis Lecture for Medical Students35特特点点：脓脓汁粘稠汁粘稠病病灶局限灶局限362015/5/7Staphylococcus- Junqis Lecture for Medical StudentsStaphylococcus systemic infectionsOsteomyelitis (骨髓炎骨髓炎) Toxemia Bacteremia Septicemia pyemiapyemia372015/5/7Staphylococc
12、us- Junqis Lecture for Medical Students烫伤样皮肤综合征烫伤样皮肤综合征 （staphylococcal Scalded Skin Syndrome, SSSS）2015/5/7Staphylococcus- Junqis Lecture for Medical Students38毒毒性性休克综合征休克综合征（ toxic shock syndrome, TSS）Following controversial test marketing in Rochester, New York and Fort Wayne, Indiana, in August
13、1978, Procter and Gamble introduced superabsorbent Rely tampons to the United States market in response to 2015/5/7Staphylococcus- Junqis Lecture for Medical Students391.Rely used carboxymethylcellulose (CMC) and compressed beads of polyester for absorption. 2.This tampon design could absorb nearly
14、20 times its own weight in fluid.3.Further, the tampon would “blossom“ into a cup shape in the vagina to hold menstrual fluids without leakage. United States market in response to womens demands for tampons that could contain an entire menstrual flow without leaking or replacement. 白带分泌物革兰氏染色：白带分泌物革
15、兰氏染色： 乳酸杆菌乳酸杆菌，G+402015/5/7Staphylococcus- Junqis Lecture for Medical Students乳酸杆菌乳酸杆菌，免疫免疫性性人人类类有一定有一定的的天天然然免疫免疫力，力，只有当只有当皮肤皮肤黏膜受黏膜受伤伤后后，宿宿主主免疫免疫力力降低时降低时，才才易易引起引起葡萄球菌感染葡萄球菌感染。引起引起葡萄球菌感染葡萄球菌感染。感染感染后后能能获得一定获得一定的的免疫免疫力，力，但不但不强，强，难以防止再次难以防止再次感染感染。412015/5/7Staphylococcus- Junqis Lecture for Medical Stu
16、dents（一一）非非特特异异性性免疫免疫： 正正常常皮肤皮肤粘膜屏障粘膜屏障作用作用 中中性性粒细粒细胞胞吞吞噬作用噬作用（二二）特特异异性性免疫免疫：（二二）特特异异性性免疫免疫： 体液体液免疫免疫调理调理素抗素抗体体促吞促吞噬作用噬作用 抗抗毒毒素抗素抗体体中中和外和外毒毒素素 细细胞胞免疫免疫422015/5/7Staphylococcus- Junqis Lecture for Medical Students标本标本脓脓汁汁，血，血液液，剩余剩余食食物，物，呕吐呕吐物物等等分分离离培养培养 血血浆浆凝固酶凝固酶直接涂片镜检直接涂片镜检革兰革兰阳阳性性葡萄球菌葡萄球菌生生长现象长现象色素，溶血色素，溶血微微生物生物
