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1、22. The chemical tool of claim 21 wherein the restriction endonuclease for targeting DNA sequences is selected from the group consisting of:Sequence cut Enzyme Name SEQ ID NO:AAR/AATTY AcsI 53R/AATTY ApoI 53A/AGCTT HindIII 54AA/CGTT Psp140 6I 55AAT/ATT SspI 56ACA/CRYGT AflIII 57A/CCGGT AgeI 58A/CATG
2、T BspLU11I 59ACCTGC(4,8) BspMI 60, 61ACTGG(1,611) BsrI 62, 63R/CCGGY BsrFI 64R/CCGGY Cfr10I 64A/CGT MaeII 65A/CGCGT MluI 66RCATG/Y NspI 67A/CCGGT PinAI 58A/CCWGGT SexAI 68A/CTAGT SpeI 69AGAGG/CCT AatI 70AG/CT AluI 71A/GATCT BglII 72R/GATCY BstYI 73RG/GNCCY DraII 74AGC/GCT Eco47III 75RG/GNCCY EcoO109
3、I 74RGCGC/Y HaeII 76RG/GWCCY PpuMI 77AGT/ACT ScaI 78AGG/CCT StuI 70R/GATCY XhoII 73ATAT/TAAT AseI 79AT/TAAT AsnI 79AT/CGAT BspDI 80AT/CGAT ClaI 80ATGCA/T NsiI 81ATTT/AAAT SwaI 82CACAGNNN/CTG AlwNI 83CAC/GTG BbrPI 84CACNNN/GTG DraIII 85YAC/GTR BsaAI 86C/AATTG MfeI 87C/AATTG MunI 87CA/TATG NdeI 88CATG
4、/ NlaIII 89CMG/CKG NspBII 90CAC/GTG PmaCI 84CAC/GTG PmlI 84CAG/CTG PvuII 91CR/CCGGYG SgrAI 92(13,9)CATCC FokI 93, 94CCC/CGC AciI 95CC/TNAGG AocI 96/CCWGG ApyI 97C/CTAGG AvrII 98C/CTAGG BinI 98C/CNNGG BsaJI 99CCNNNNN/NNGG BsiYI 23CCNNNNN/NNGG BslI 23CCANNNNN/NTGG BssGI 27CC/WGG BstNI 100CCANNNNN/NTGG
5、 BstXI 27CC/TNAGG Bsu36I 96C/CRYGG DsaI 101C/YCGRG AvaI 102CCTNN/NNNAGG EcoNI 32/CCWGG EcoRII 97C/CGG HpaII 103CCGC/GG KspI 104CCTC(7,6) MnlI 105, 106C/CGG MspI 103CC/TNAGG MstII 96CC/WGG MvaI 100CC/SGG NciI 107C/CATGG NcoI 108CMG/CKG NspBII 90CCANNNN/NTGG PflMI 44CCGC/GG SacII 104CC/TNAGG SauI 96CC
6、/NGG ScrFI 109CCC/GGG SmaI 110CCTGCA/GG Sse8387I 111CCGC/GG SstII 104C/CWWGG StyI 112CCANNNN/NTGG Van91I 44CCANNNNN/NNNNTGG XcmI 50C/CCGGG XmaI 113C/CCGGG XmaCI 113(1,611)CCAGT BsrI 114, 115CG(10,12)CGANNNNNNTGC(12,10) BcgI 116, 117CGRY/CG BsiEI 118C/GTACG BsiWI 119CG/CG BstUI 120C/GGCCG EagI 121Y/G
7、GCCR CfrI 122Y/GGCCR EaeI 122C/GGCCG EclXI 121CGTCTC(1,5) Esp3I 123, 124CG/CG FnuDII 120CG/CG MvnI 120CGAT/CG PvuI 125CG/GWCCG RsrII 126CR/CCGGYG SgrAI 92CG/CG ThaI 120C/GGCCG XmaIII 121CTC/TTAAG AflII 127C/TAG BfaI 128C/TTAAG BfrI 127CTGGAG(16,14) BpmI 129, 130C/TNAG DdeI 131CTCTTC(1,4) EarI 132, 1
8、33C/YCGRG AvaI 102CTGAAG(16,14) Eco57I 134, 135CTGGAG(16,14) GsuI 129, 130C/TAG MaeI 128C/TCGAG PaeR7I 136CTGCA/G PstI 137C/TAG RmaI 128C/TRYAG SfcI 138C/TCGAG XhoI 136(14,16)CTCCAG BpmI 139, 140(14,16)CTGCAC BsgI 141, 142(14,16)CTTCAG Eco57I 143, 144(14,16)CTCCAG GsuI 139, 140GAGACGT/C AatII 145GAC
9、N/NNGTC AspI 146GAANN/NNTTC Asp700 8GACNNN/NNGTC AspEI 9GAAGAC(2,6) BbsI 147, 148GAAGAC(2,6) BpuAI 147, 148GATNN/NNATC BsaBI 20GAATGC(1,611) BsmI 149, 150GA/TC DpnI only if 151G-Me/GATC DpnII 151GACNNNN/NNGTC DrdI 28GACNNN/NNGTC Eam1105I 9GAG/CTC Ecl136II 152R/AATTY AcsI 53GR/CGYC AcyI 153GR/CGYC Ah
10、aII 153R/AATTY ApoI 53GWGCW/C AspHI 154GRGCY/C BanII 155GDGCH/C BmyI 156GR/CGYC BsaHI 153GDGCH/C Bsp1286I 156G/AATTC EcoRI 157GAT/ATC EcoRV 158GACGC(5,10) HgaI 159, 160,GWGCW/C HgiAI 154G/ANTC HinfI 161GATNN/NNATC MamI 20/GATC MboI 151GAAGA(8,7) MboII 162, 163/GATC NdeII 151GDGCH/C NspII 156GAGTC(4,
11、5) PleI 164, 165GAGCT/C SacI 166/GATC Sau3AI 151GAGCT/C SstI 166G/AWTC TfiI 167GACN/NNGTC Tth111I 146GAANN/NNTTC XmnI 8(9,5)GATGC SfaNI 168, 169(5,4)GATCC AlwI 170, 171(5,1)GAGACC BsaI 172, 173(5,1)GAGAC BsmAI 174, 175(4,1)GAAGAG EarI 176, 177(5,1)GAGACG Esp3I 178, 179(6,7)GAGG MnlI 180, 181(5,4)GAC
12、TC PleI 182, 183GCGCAGC(8,12) BbvI 184, 185GCCNNNN/NGGC BglI 15GC/TNAGC Bpu1102I 186G/CGCGC BsePI 187G/CGCGC BssHII 187GC/TNAGC CelII 186GCG/C CfoI 188R/CCGGY BsrFI 64R/CCGGY Cfr10I 64GC/TNAGC EspI 186GC/NGC Fnu4HI 189GCG/C HhaI 188G/CGC HinPI 188GC/NGC ItaI 189GCC/GGC NaeI 190G/CCGGC NgoMI 191G/CTA
13、GC NheI 192GC/GGCCGC NotI 193RCATG/Y NspI 67GCATC(5,9) SfaNI 194, 195GCATG/C SphI 196GCCC/GGGC SrfI 197G/CGG AciI 198(12,8)GCTGC BbvI 199, 200(10,12)GCANNNNNNTCG(12,10) BcgI 201, 202(1,1)GCATTC BsmI 203, 204(8,4)GCAGGT BspMI 205, 206(10,5)GCGTC HgaI 207, 208GGG/GTACC Acc65I 209G/GWCC AflI 210GGATC(4
14、,5) AlwI 211, 212GGGCC/C ApaI 213GG/CGCGCC AscI 214G/GTACC Asp718 209G/GWCC AvaII 210G/GATCC BamHI 215G/GYRCC BanI 216GGTCTC(1,5) BsaI 217, 218G/GTNACC BstEII 219R/GATCY BstYI 73GR/CGYC AcyI 153GR/CGYC AhaII 153GRGCY/C BanII 155GDGCH/C BmyI 156GR/CGYC BsaHI 153GDGCH/C Bsp1286I 156RG/GNCCY DraII 74RG
15、/GNCCY EcoO109I 74GGATG(9,13) FokI 220, 221GGCCGG/CC FseI 222RGCGC/Y HaeII 76GG/CC HaeIII 223GGTGA(8,7) HphI 224, 225G/GCGCC KasI 226GGTAC/C KpnI 227GG/CGCC NarI 228GGN/NCC NlaIV 229GDGCH/C NspII 156RG/GWCCY PpuMI 77G/GNCC Sau96I 230GGCCNNNN/NGGCC SfiI 49R/GATCY XhoII 73GTGT/MKAC AccI 231G/TGCAC Alw
16、44I 232G/TGCAC ApaLI 232GTGCAG(16,14) BsgI 233, 234GTCTC(1,5) BsmAI 235, 236GTA/TAC Bst1107I 237GWGCW/C AspHI 154GDGCH/C BmyI 156GDGCH/C Bsp1286I 156GWGCW/C HgiAI 154GTY/RAC HincII 238GTY/RAC HindII 238GTT/AAC HpaI 239/GTNAC MaeIII 240GDGCH/C NspII 156GTTT/AAAC PmeI 241GT/AC RsaI 242G/TCGAC SalI 243G/TGCAC SnoI 232(6,2)GTCTTC BbsI 244, 245(6,2)GTCTTC BpuAI 244, 245TAYAC/GTR BsaAI 86TAC/GTA SnaBI 246TCT/CCGGA AccII 247T/CCGGA BseAI 247T/CCGGA BspEI 247T/CA
