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1、常用荧光团的参数与选择2015/4/17Fluorophore PeakExcitation (nm)PeakEmission (nm)Intersection (nm) TypeCenterWavelength(nm)Optical Density ODBandwith(nm)MinimumTransmission(%)Excitation 377 6 50 851,8-ANS (BSA) 374.9 477 422.9Emission 480 3 10 45Excitation 580 3 10 452-dodecylresorufin (lipid) 581.9 595 589.5Emi
2、ssion 593 6 40 90Excitation 505 3 10 455-(and-6)-carboxy-2,7-dichlorofluorescein (pH 9.0) 504 524.9 513.8Emission 520 6 35 90Excitation 543 6 22 905-carboxytetramethylrhodamine 5-TAMRA (MeOH) 542.9 567 554.4Emission 568 3 10 45Excitation 492 3 10 455-chloromethylfluorescein (pH 7.2) 490.9 517 501.8E
3、mission 515 3 10 45Excitation 492 3 10 455-FAM (pH 9.0) 491.9 518.1 503.5Emission 520 6 35 90Excitation 580 3 10 455-ROX (pH 7.0) 578.2 604 589.7Emission 600 3 80 605-TAMRA (pH 7.0) 553 576 563.4 Excitatio 550 3 80 60nEmission 580 3 10 45Excitation 520 6 35 906-carboxyrhodamine 6G (pH 7.0) 525 547 5
4、36Emission 546 3 10 45Excitation 520 6 35 906-JOE (pH 9.0) 519.8 548 532.7Emission 550 3 80 60Excitation 340 6 26 757-amino-4-methylcoumarin (pH 7.0) 346 442 396.5Emission 442 3 10 40Excitation 550 3 80 607-aminoactinomycin D (DNA) 548.1 647.9 601.8Emission 647 3 10 45Excitation 365 3 10 257-hydroxy
5、-4-methylcoumarin (pH 9.0) 360.1 447.9 403Emission 447 6 60 90Excitation 500 6 24 90Acridine orange (DNA) 502.1 526 513Emission 531 6 40 90Excitation 340 6 26 75Alexa Fluor 350 goat anti-mouse IgG antibody (pH 8.0) 343 441 392.9Emission 442 3 10 40Excitation 400 3 50 55Alexa Fluor 405 goat anti-mous
6、e IgG antibody (pH 7.2) 401.1 422.1 409Emission 420 3 10 40Alexa Fluor 430 goat anti-mouse IgG antibody (pH 7.2) 431.2 540 492.9 Excitation 430 3 10 40Emission 540 3 10 45Excitation 492 3 10 45Alexa Fluor 488 dye-labeled oligonucleotide (H2O) 493.1 519 505.8Emission 520 6 35 90Excitation 500 6 24 90
7、Alexa Fluor 488 goat anti-mouse IgG antibody (pH 8.0) 498.9 520 508.8Emission 520 6 35 90Excitation 492 3 10 45Alexa Fluor 488 hydrazide (H2O) 492.8 517.1 504.9Emission 515 3 10 45Excitation 520 6 35 90Alexa Fluor 514 goat anti-mouse IgG antibody (pH 8.0) 518 542.9 529.8Emission 543 6 22 90Excitatio
8、n 535 3 10 45Alexa Fluor 532 goat anti-mouse IgG antibody (pH 7.2) 533.9 553 543Emission 550 3 80 60Excitation 550 3 80 60Alexa Fluor 546 dye-labeled oligonucleotide (H2O) 554.1 574 563.8Emission 580 3 10 45Excitation 562 6 40 90Alexa Fluor 546 goat anti-mouse IgG antibody (pH 7.2) 561.3 572 566.9Em
9、ission 568 3 10 45Excitation 550 3 80 60Alexa Fluor 555 dye-labeled oligonucleotide (H2O) 552.3 567.1 559.6Emission 568 3 10 45Excitation 550 3 80 60Alexa Fluor 555 goat anti-mouse IgG antibody (pH 7.2) 553.2 567.9 560.2Emission 568 3 10 45Excitation 550 3 80 60Alexa Fluor 555 hydrazide (H2O) 551.1
10、564 557.1Emission 562 6 40 90Excitation 580 3 10 45Alexa Fluor 568 goat anti-mouse IgG antibody (pH 7.2) 578.9 603 590.2Emission 600 3 80 60Excitation 568 3 10 45Alexa Fluor 568 hydrazide (H2O) 572.9 599.9 585.8Emission 600 3 80 60Excitation 589 3 10 45Alexa Fluor 594 cadaverine (H2O) 587.9 614.1 60
11、0.1Emission 610 3 10 45Excitation 589 3 10 45Alexa Fluor 594 dye-labeled oligonucleotide (H2O) 590.9 621 605.7Emission 620 3 10 45Excitation 589 3 10 45Alexa Fluor 594 goat anti-mouse IgG antibody (pH 7.2) 590.2 618.1 604.2Emission 620 3 10 45Excitation 610 3 10 45Alexa Fluor 610 goat anti-mouse IgG
12、 antibody (pH 7.2) 611.9 630 620.2Emission 628 6 40 90Excitation 568 3 10 45Alexa Fluor 610-R-phycoerythrin streptavidin (pH 7.2) 566.9 627 589.7Emission 628 6 40 90Excitation 632 4 10 85Alexa Fluor 633 goat anti-mouse IgG antibody (pH 7.2) 631.1 649.9 640.2Emission 650 4 10 85Alexa Fluor 635 goat a
13、nti-mouse IgG antibody (pH 7.2) 633.1 647.9 639.9 Excitatio 632 4 10 85nEmission 647 3 10 45Excitation 650 4 10 85Alexa Fluor 647 dye-labeled oligonucleotide (H2O) 650.1 667.9 659.1Emission 671 4 10 85Excitation 650 4 10 85Alexa Fluor 647 goat anti-mouse IgG antibody (pH 7.2) 649.9 671 660.6Emission
14、 671 4 10 85Excitation 650 4 10 85Alexa Fluor 647 hydrazide (H2O) 649.1 670 659.2Emission 671 4 10 85Excitation 568 3 10 45Alexa Fluor 647-R-phycoerythrin streptavidin (pH 7.2) 568.2 666 648.9Emission 671 4 10 85Excitation 656 4 10 85Alexa Fluor 660 goat anti-mouse IgG antibody (pH 7.2) 663.1 690.9
15、679.3Emission 690 3 10 50Excitation 676 3 10 50Alexa Fluor 680 goat anti-mouse IgG antibody (pH 7.2) 680.9 703.9 692.8Emission 700 3 80 60Excitation 568 3 10 45Alexa Fluor 680-R-phycoerythrin streptavidin (pH 7.2) 569.6 702 687.1Emission 700 3 80 60Excitation 694 3 10 50Alexa Fluor 700 goat anti-mou
16、se IgG antibody (pH 7.2) 695.9 719 708.3Emission 730 3 10 50Alexa Fluor 750 goat anti-mouse IgG antibody (pH 7.2) 752.4 776.1 763.6 Excitation 766 3 10 50Emission 780 3 10 50Excitation 780 3 10 50Alexa Fluor 790 goat anti-mouse IgG antibody (pH 7.2) 781.8 804.9 791.1Emission 800 3 10 50Excitation 340 6 26 75Alexa Fluorr 350 346.9 443.9 394.2Emission 442 3 10 40Excitation 650 4 10 85Allophycocyanin (pH 7.5) 651 660.1 655.6Emission 656 4 10 85Excitation 568 3 10 45A
