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1、1细胞膜钙 ATP 酶在心肌缺血/ 再灌注和腺苷诱导预适应中的作用【摘要】 目的：探讨在心肌缺血/再灌注和腺苷诱导预适应过程中细胞膜钙泵（PMCA）的可能作用。方法：将培养的乳鼠心肌细胞分为 3 组：缺血/再灌注组、腺苷预处理组和对照组。各组乳酸脱氢酶（LDH ）漏出量以生化法检测（n5） ；半定量 RTPCR 检测各组 PMCA 基因转录水平（ n3 ） ；PMCA 活性以定磷比色法测定（n 5 ） 。结果：（ 1）缺血/再灌注组 LDH 漏出量明显增高(P0.001)，腺苷预处理组 LDH 漏出量显著降低(P0.05)；（3）各组间 PMCA 蛋白活性无显著性差异（ P0.05)。结论：在
2、乳鼠心肌细胞缺血/再灌注过程中 PMCA 无代偿功能；PMCA 不直接参与腺苷诱导预适应的心肌保护作用。 【关键词】 钙 ATP 酶 钙 再灌注损伤 药物预处理 大鼠 SpragueDawleyAbstract:Objective To study the effectiveness of interstitial irradiation by 32Pchromic phosphate (32Pcolloid) on the latent metastasis during lung cancer resection. Methods 73 patients with lung cancer
3、underwent resection of tumor and 2interstitial administration of 32 Pcolloid, by the same time span, 58 patients with same diagnosis underwent operation served as control group. After operation the dynamic distribution of body surface 32Pcolloid activity, incidence of complication, rates of supracla
4、vicle lymph node (SCL) metastasis in different pathologic patterns, and survival rates at 1,3,5 year were studied. Results No operative death occurred in these two groups. The pathologic patterns, incidences of lymph node metastasis and the incidences of major complication after operation were no si
5、gnificant differences between these two groups (20.0031.696,P0.05). The incidence of postoperative SCL metastasis in surgery plus 32Pcolloid group was significantly lower than that in surgery group（24.5075.348,P0.05 or P0.05), but the differences between the two groups of the 3,5year survival rates
6、were significantly 3(24.207,3.997,P0.05). Conclusion Interstitial injection of 32Pcolloid during resection of lung cancer is a safe and effective procedure in controlling the latent lymphatic metastasis and local recurrence, and is surely to prolong the survival time of patients.Key words：lung cance
7、r; lymphatic metastasis; operative treatment; radiotherapy; 32Pcolloid; phosphorus radioisotopesCLC number R734.2; R817.541 Document code A Article ID 16716264(2008)04024406China is one of the countries in Asia where smoking rates are now high and where lung cancer is becoming more and more frequent
8、 as a consequence. In order to give patients with lung cancer a better therapy, many methods have been tried including improvement of surgical resection, a more thoroughly intrapulmonary lymph nodes dissection, and a better chemotherapy and radiotherapy12 . Interstitial irradiation using 32Pchromic
9、phosphate (32Pcolloid) on the latent metastasis during lung cancer resection is a new 4method and has never been reported. From March 1997 to March 2001, we randomly choose 103 patients who underwent resection of tumor and gave them the interstitial injection of 32 Pcolloid by the same time span. We
10、 choose 73 of these patients who had integrated data and 58 patients with same diagnosis underwent operation served as control group. Incidence of complication, rates of supraclavicle lymph node (SCL) metastasis in different pathologic patterns, and survival rates at 1,3,5year were studied, and the
11、results are as follows.1 Materials and Methods1.1 SubjectsIn surgery plus 32Pcolloid group there were 73 patients, including 48 males and 25 females, aged 32-83 years (53.5 years in average ). The pathologic patterns in these patients were squamous cell carcinoma 48 cases(65.8%), adenocarcinoma 15 c
12、ases (20.5%) and small cell carcinoma 10 cases (13.7%). There were 58 patients in the control group who had only undergone surgical therapy including 33 males 5and 25 females, aged 36-74 years(51.5 years in average ). Their pathologic patterns were squamous cell carcinoma 40 cases (69.0%), adenocarc
13、inoma 13 cases (22.4 % )and small cell carcinoma 5 cases(8.6%). The primary disease of all patients in the two groups were diagnosed after the examinations of chest Xray, chest CT, fibre bronchoscope and sputum exfoliative cell examination before surgery. All routine examinations and Karnofsky score
14、 before surgery pointed out that all these patients were able to endure thoracotomy. Followup studies were performed in the third and the sixth month after discharged from hospital, then once every half year. On the first follow up visit, chest Xray examination, chest CT or related serological exami
15、nation were taken, thereafter inquired patients quality of life. The followup period was 12-60 months.1.2 Operation methodsAll patients of these two groups underwent thoracotomy either unilateral pneumonectomy or lobectomy with resection of visible lymph nodes. The lymph node of homolateral mediastimun were explored and carried out 6pathological examin
