褪黑素与乳腺癌大鼠外周血Treg相关细胞因子的关系研究

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1、褪黑素与乳腺癌大鼠外周血 Treg 相关细胞因子的关系研究【摘要】 目的：研究褪黑素与乳腺癌大鼠外周血血清中转化生长因子 1（Transforming growth factor-1，TGF-1）和白介素 35（Interleukin-35，IL-35）水平的关系，分析褪黑素对乳腺癌大鼠外周血中调节性 T 细胞（Regulatory T cell，Treg cell）相关细胞因子及肿瘤免疫的影响。方法：选用两月龄雌性 SD 大鼠 60 只，接种大鼠乳腺癌 SHZ-88 细胞，按照随机数字表法分成实验组（注射褪黑素）和对照组（注射生理盐水） ，10 d 后乳腺癌大鼠荷瘤动物模型建成，抽取大鼠股动

2、脉外周血，采用ELISA 法检测外周血血清中 TGF-1、IL-35 的浓度。结果：实验组乳腺癌大鼠外周血 TGF-1 浓度低于对照组，差异有统计学意义（P【关键词】 褪黑素； 乳腺癌； Treg 细胞； 转化生长因子 1； 白介素 35 Study on Relationship between Melatonin and Treg Related Cytokines in Peripheral Blood of Rats with Breast Cancer/HUANG Yu-dian，ZHENG Xi，XUE Yu-qin，et al./Medical Innovation of Chi

3、na，2017，14（11）：028-030 【Abstract】 Objective：To study the relationship between melatonin and expression levels of transforming growth factor-1 and interleukin-35 in peripheral blood serum of breast cancer rats，and to analyze the effect of melatonin on regulatory T cell related cytokines in peripheral

4、 blood and tumor immunity of breast cancer rats.Method：A total of 60 female SD rats in age 2 months were selected，and the rat breast cancer SHZ-88 cells were inoculated in the rats.The rats were randomly divided to the experimental group injected with Melatonin and the control group injected with sa

5、line.The tumor bearing animal models of breast cancer rats were established on the 10th day after inoculating.The peripheral blood of rats femoral artery，and the concentrations of TGF-1 and IL-35 in peripheral blood serum were detected by ELISA method.Result：In the experimental group，the concentrati

6、on of TGF-1 in peripheral blood of breast cancer rats was lower than that of the control group（P 1 材料与方法 1.1 实验动物和试剂 选用两月龄雌性 SD 大鼠 60 只，体重范围（20010）g，标准饲养。按照随机数字表法分为两组，分别为注射生理盐水的对照组和按 15 mg/（kg?d）注射褪黑素的实验组，每组 30 只。大鼠乳腺癌 SHZ-88 细胞购于上海生命科学院，褪黑素购于美国Sigma 公司，TGF-1 和 IL-35 的 ELISA 试剂盒购于美国R&D 公司。 1.2 建立动物模

7、型 乳腺癌细胞株于培养瓶中传代培养，当癌细胞培养至对数生长期时，吹打制成单细胞悬液，每只大鼠按 5105 个细胞量在大腿内侧根部皮下接种 SHZ-88乳腺癌单细胞悬液， 1 次/只。自接种日起，每日 1800 分别按对照组和实验组的不同干预手段，给予腹腔内注射生理盐水或 15 mg/（kg?d）的褪黑素，1 次/d。接种后第 10 天可在接种处触及皮下瘤块，抽取另一侧大腿股动脉外周血待检测，处死荷乳腺癌大鼠，切除接种处瘤块并经病理切片观察证实为乳腺癌。 1.3 ELISA 法检测 采用双抗体夹心 ELISA 法检测大鼠外周血血清中 TGF-1、IL-35 的水平，操作严格按照ELISA 试剂盒

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