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1、Dataset S2. Molecular analysis of the medfly sperm-marked strain 1260_F-3_m-1 for verifying transgene stabilization1) Original genomic situation at the integration site of the 5 pBac endOriginal strain 1260_F-3_m-1: primer pair 380/229ggcccacatttgtctaacttctgttacgtggttcagtagtgagttttgagtaatagcctaaatgt
2、agatgtttaaCCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGGAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTGTGCATTTAGGACATCTCAGTCGCCGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTG2) Verification of the site-specific integration in 1260_F-3_m-1attR-site of the integrated strain int_1260_F-3_m-1: primer pair 373/
3、372ATCTTGACCTTGCCACAGAGGACTATTAGAGGTAAGAATAAACATTGTTGGTCAACTTCAAAGTCCACGAGGCGTAGCCGAGTCTCTGCACTGAACATTGTCAGATCTGGCTAAATGGTATGGCAAGAAAAGGTATGCAATATAATAATCTTTTATTGGGTATGCAACGAAAATTTGTTTCGTCAACGTATGCAATATTTTTTATTAAAAGAGGGTATGCAATGTATTTTATTAAAAACGGGTATGCAATATAATAATCTTTTATTGGGTATGCAACGAAAATTTGTTTCGTCAAAG
4、TATGCAATATTTTTTATTAAAAGAGGGTATGCAATGTATTTTATTAAAAACGGGTATGCAATAAAAAATTATTTGGTTTCTCTAAAAAGTATGCAGCACTTATTTTTTGATAAGGTATGCAACAAAATTTTACTTTGCCGAAAATATGCAATGTTTTTGCGAATAGGGTACCGGCGCGCCTAGGCCGGCCGAATTCGTGCCCCAACTGGGGTAACCTTTGGGCTCCCCGGGCGCGTACTCCACCTCACAGATCTCGAGCTCAAattL-site of the integrated strain in
5、t_1260_F-3_m-1: primer pair 330/382GCTCATCGACTTGATATTGTCCGACACATTTTCGTCGATTTGCGTTTTGATCAAAGACTTGAGCAGAGACACGTTAATCAACTGTTCAAATTGATCCATATTAACGATGGGCCCGGTACCCTATTCGCAAAAACATTGCATATTTTCGGCAAAGTAAAATTTTGTTGCATACCTTATCAAAAAATAAGTGCTGCATACTTTTTAGAGAAACCAAATAACTTTTTATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCT
6、TTTAATAAAAAATATTGCATACTTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACGTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCTTTTCTTGCCATACCATTTAGCCGGATCCTGCGGGTGCCAGGGCGTGCCCTTGAGTTCTCTCAGTTGGGGGCGTAGGGTCAAT3) Verification of remobilizat
7、ion and loss of piggyBac ends Stabilized strain stab_1260_F-3_m-1: primer pair 380/382ggcccacatttgtctaacttctgttacgtggttcagtagtgagttttgagtaatagcctaaatgtagatgtttaaATAATAGTTTCTAATTTTTTTATTATTCAGCCTGCTGTCGTGAATACCGTATATCTCAACGCTGTCTGTGAGATTGTCGTATTCTAGCCTTTTTAGTTTTTCGCTCATCGACTTGATATTGTCCGACACATTTTCGTCG
8、ATTTGCGTTTTGATCAAAGACTTGAGCAGAGACACGTTAATCAACTGTTCAAATTGATCCATATTAACGATGGGCCCGGTACCCTATTCGCAAAAACATTGCATATTTTCGGCAAAGTAAAATTTTGTTGCATACCTTATCAAAAAATAAGTGCTGCATACTTTTTAGAGAAACCAAATAATTTTTTATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACTTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACGTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCTTTTCTTGCCATACCATTTAGCCGGATCCTGCGGGTGCCAGGGCGTGCCCTTGAGTTCTCTCAGTTGGGGGCGTAGGGTCAAT
