16ÕÈnÁh )16S rRNA u×ÉÄ¥y ë康素明△,潘忠诚,张玉魁,何群,马佳明,赵雨杰(ÏSD SvÐ$DÐý3þ ËÏ, a+ 110001)【K1】 "¥:通过建立 16种常见致病菌的进化树,了解细菌之间的进化关系,为设计其诊断用芯片的特异探针提供理论基础ZE:常规方法提取细菌 DNA, PCR对称扩增后进行 DNA测序,利用 BLAST和 ClustaWl 程序分析测序结果并建立进化树²T:20种细菌中, 16种成功扩增出 PCR产物,得到了理想的测序结果,同时建立了这 16种细菌的进化树²:根据进化树所示,可以设计不同细菌的特异诊断用探针,利用芯片快速鉴定细菌1oM】细菌;进化树;诊断芯片【Ïms Ë|】Q811.4 【ÓDSM】A 【ÓcI|】0258 -4646(2005)03 - 0208- 02Phyligeneticanalysison the16S rRNA region of16 common nosogeneticbacteriaKANG Su-ming, PAN Zhong-cheng, ZHANG Yu-kui, HE Qun, MA Jia-ming, ZHAO Yu-jie(Center ofBiochip, College ofBasicM edicalSciences, ChinaM edicalUniversity, Shenyang 110001, China)【Abstract】 Objective:To understand the phyligenetic relationship between different bacteria by establishing thephyligenetic tree of16 common nosogeneticbacteria, and to provide theoretical basis for designing special probes in diag-nosticm icroarray forbacteria. Methods:Routinemethod was used to extract thebacteriaDNA. TheDNA sequencingwasperformed after the polymerase chain reaction (PCR). By using theBLAST and ClustaWl program, the sequencing resultswere analyzed and thephyligenetic treewas established. Results:Among the 20 kinds of bacteria, we obtained successfulPCR results for16 kinds of bacteria. The sequencing results and the phyligenetic treewere idea.l Conclusion:Based onthe phyligenetic analysis, specialprobes for differentiating bacteria can be designed successfully. It is viable to identifyingdifferentbacteria through DNA m icroarray.【Keywords】 bacteria;phyligenetic tree;diagnosticmicroarrayÉÄ9ëÕ"(phyligenetic tree),Ç V¨¿Y{9] Ë 3þW¥ÉÄ1",]HVYVós¦ªQ êm,QþÕW¥ÉÄ Ö。
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